Capillary Electrophoresis of Carbohydrates:
Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques i...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2003
|
| Schriftenreihe: | Methods in Molecular Biology™
213 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods. Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis |
| Beschreibung: | 1 Online-Ressource (XIV, 318 p) |
| ISBN: | 9781592592944 |
| DOI: | 10.1385/1592592945 |
Internformat
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| 520 | |a Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods. Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis | ||
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Datensatz im Suchindex
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| spelling | Capillary Electrophoresis of Carbohydrates edited by Pierre Thibault, Susumu Honda Totowa, NJ Humana Press 2003 1 Online-Ressource (XIV, 318 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 213 Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods. Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis Life Sciences Biochemistry, general Carbohydrate Chemistry Life sciences Carbohydrates Biochemistry Elektrophorese (DE-588)4014373-9 gnd rswk-swf Kohlenhydrate (DE-588)4164517-0 gnd rswk-swf Kapillarelektrophorese (DE-588)4290002-5 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Kohlenhydrate (DE-588)4164517-0 s Elektrophorese (DE-588)4014373-9 s DE-604 Kapillarelektrophorese (DE-588)4290002-5 s 2\p DE-604 Thibault, Pierre edt Honda, Susumu edt Erscheint auch als Druck-Ausgabe 9780896038264 https://doi.org/10.1385/1592592945 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Capillary Electrophoresis of Carbohydrates Life Sciences Biochemistry, general Carbohydrate Chemistry Life sciences Carbohydrates Biochemistry Elektrophorese (DE-588)4014373-9 gnd Kohlenhydrate (DE-588)4164517-0 gnd Kapillarelektrophorese (DE-588)4290002-5 gnd |
| subject_GND | (DE-588)4014373-9 (DE-588)4164517-0 (DE-588)4290002-5 (DE-588)4143413-4 |
| title | Capillary Electrophoresis of Carbohydrates |
| title_auth | Capillary Electrophoresis of Carbohydrates |
| title_exact_search | Capillary Electrophoresis of Carbohydrates |
| title_full | Capillary Electrophoresis of Carbohydrates edited by Pierre Thibault, Susumu Honda |
| title_fullStr | Capillary Electrophoresis of Carbohydrates edited by Pierre Thibault, Susumu Honda |
| title_full_unstemmed | Capillary Electrophoresis of Carbohydrates edited by Pierre Thibault, Susumu Honda |
| title_short | Capillary Electrophoresis of Carbohydrates |
| title_sort | capillary electrophoresis of carbohydrates |
| topic | Life Sciences Biochemistry, general Carbohydrate Chemistry Life sciences Carbohydrates Biochemistry Elektrophorese (DE-588)4014373-9 gnd Kohlenhydrate (DE-588)4164517-0 gnd Kapillarelektrophorese (DE-588)4290002-5 gnd |
| topic_facet | Life Sciences Biochemistry, general Carbohydrate Chemistry Life sciences Carbohydrates Biochemistry Elektrophorese Kohlenhydrate Kapillarelektrophorese Aufsatzsammlung |
| url | https://doi.org/10.1385/1592592945 |
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