Recombinant Protein Production in Yeast: Methods and Protocols
In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promot...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2012
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
866 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host |
| Beschreibung: | 1 Online-Ressource (XII, 248 p. 47 illus., 6 illus. in color) |
| ISBN: | 9781617797705 |
| DOI: | 10.1007/978-1-61779-770-5 |
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| isbn | 9781617797705 |
| language | English |
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| physical | 1 Online-Ressource (XII, 248 p. 47 illus., 6 illus. in color) |
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| spelling | Recombinant Protein Production in Yeast Methods and Protocols edited by Roslyn M. Bill Totowa, NJ Humana Press 2012 1 Online-Ressource (XII, 248 p. 47 illus., 6 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 866 In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host Biomedicine Human Genetics Medicine Human genetics Bill, Roslyn M. edt Erscheint auch als Druck-Ausgabe 9781617797699 https://doi.org/10.1007/978-1-61779-770-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Recombinant Protein Production in Yeast Methods and Protocols Biomedicine Human Genetics Medicine Human genetics |
| title | Recombinant Protein Production in Yeast Methods and Protocols |
| title_auth | Recombinant Protein Production in Yeast Methods and Protocols |
| title_exact_search | Recombinant Protein Production in Yeast Methods and Protocols |
| title_full | Recombinant Protein Production in Yeast Methods and Protocols edited by Roslyn M. Bill |
| title_fullStr | Recombinant Protein Production in Yeast Methods and Protocols edited by Roslyn M. Bill |
| title_full_unstemmed | Recombinant Protein Production in Yeast Methods and Protocols edited by Roslyn M. Bill |
| title_short | Recombinant Protein Production in Yeast |
| title_sort | recombinant protein production in yeast methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics |
| url | https://doi.org/10.1007/978-1-61779-770-5 |
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