Matrix Metalloproteinase Protocols:
Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode w...
Gespeichert in:
Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2010
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Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
622 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume |
Beschreibung: | 1 Online-Ressource (XII, 472 p) |
ISBN: | 9781603272995 |
DOI: | 10.1007/978-1-60327-299-5 |
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dewey-ones | 572 - Biochemistry |
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indexdate | 2024-07-10T08:05:33Z |
institution | BVB |
isbn | 9781603272995 |
language | English |
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physical | 1 Online-Ressource (XII, 472 p) |
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publishDate | 2010 |
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publisher | Humana Press |
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spelling | Matrix Metalloproteinase Protocols edited by Ian M. Clark Totowa, NJ Humana Press 2010 1 Online-Ressource (XII, 472 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 622 Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume Life Sciences Biochemistry, general Science, general Enzymology Life sciences Science Biochemistry Metalloproteinasen (DE-588)4284137-9 gnd rswk-swf Grundsubstanz (DE-588)4224942-9 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Metalloproteinasen (DE-588)4284137-9 s Grundsubstanz (DE-588)4224942-9 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s 1\p DE-604 Clark, Ian M. edt Erscheint auch als Druck-Ausgabe 9781603272988 https://doi.org/10.1007/978-1-60327-299-5 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Matrix Metalloproteinase Protocols Life Sciences Biochemistry, general Science, general Enzymology Life sciences Science Biochemistry Metalloproteinasen (DE-588)4284137-9 gnd Grundsubstanz (DE-588)4224942-9 gnd Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd |
subject_GND | (DE-588)4284137-9 (DE-588)4224942-9 (DE-588)4039983-7 (DE-588)4038971-6 |
title | Matrix Metalloproteinase Protocols |
title_auth | Matrix Metalloproteinase Protocols |
title_exact_search | Matrix Metalloproteinase Protocols |
title_full | Matrix Metalloproteinase Protocols edited by Ian M. Clark |
title_fullStr | Matrix Metalloproteinase Protocols edited by Ian M. Clark |
title_full_unstemmed | Matrix Metalloproteinase Protocols edited by Ian M. Clark |
title_short | Matrix Metalloproteinase Protocols |
title_sort | matrix metalloproteinase protocols |
topic | Life Sciences Biochemistry, general Science, general Enzymology Life sciences Science Biochemistry Metalloproteinasen (DE-588)4284137-9 gnd Grundsubstanz (DE-588)4224942-9 gnd Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd |
topic_facet | Life Sciences Biochemistry, general Science, general Enzymology Life sciences Science Biochemistry Metalloproteinasen Grundsubstanz Molekularbiologie Methode |
url | https://doi.org/10.1007/978-1-60327-299-5 |
work_keys_str_mv | AT clarkianm matrixmetalloproteinaseprotocols |