Membrane Proteomics: Methods and Protocols
With the great cellular and therapeutic importance of plasma membrane proteins, unbiased technologies such as proteomics become even more vital as they have the power to define patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disea...
Gespeichert in:
Weitere Verfasser: | , |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2009
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Schriftenreihe: | Methods in Molecular Biology™
528 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
Zusammenfassung: | With the great cellular and therapeutic importance of plasma membrane proteins, unbiased technologies such as proteomics become even more vital as they have the power to define patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. In Membrane Proteomics: Methods and Protocols, leading experts in the field compile a laboratory bench resource which provides a comprehensive toolkit of proven methods. The volume delves into various modifications to standard two-dimensional gel electrophoresis protocols as well as liquid chromatographic methods, protocols for the enrichment of diverse classes of plasma membrane proteins, in silico approaches, and cutting-edge techniques for quantitative membrane protein profiling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-use, Membrane Proteomics: Methods and Protocols is an ideal guide for investigators wishing to apply state of the art membrane proteomic methodologies in their own research programs |
Beschreibung: | 1 Online-Ressource (XIII, 342 p) |
ISBN: | 9781603273107 |
DOI: | 10.1007/978-1-60327-310-7 |
Internformat
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spelling | Membrane Proteomics Methods and Protocols edited by Matthew J. Peirce, Robin Wait Totowa, NJ Humana Press 2009 1 Online-Ressource (XIII, 342 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 528 With the great cellular and therapeutic importance of plasma membrane proteins, unbiased technologies such as proteomics become even more vital as they have the power to define patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. In Membrane Proteomics: Methods and Protocols, leading experts in the field compile a laboratory bench resource which provides a comprehensive toolkit of proven methods. The volume delves into various modifications to standard two-dimensional gel electrophoresis protocols as well as liquid chromatographic methods, protocols for the enrichment of diverse classes of plasma membrane proteins, in silico approaches, and cutting-edge techniques for quantitative membrane protein profiling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-use, Membrane Proteomics: Methods and Protocols is an ideal guide for investigators wishing to apply state of the art membrane proteomic methodologies in their own research programs Life Sciences Biochemistry, general Protein Science Membrane Biology Life sciences Biochemistry Proteins Cell membranes Membranproteine (DE-588)4130026-9 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Membranproteine (DE-588)4130026-9 s Labortechnik (DE-588)4123602-6 s b DE-604 Peirce, Matthew J. edt Wait, Robin edt Erscheint auch als Druck-Ausgabe 9781603273091 https://doi.org/10.1007/978-1-60327-310-7 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Membrane Proteomics Methods and Protocols Life Sciences Biochemistry, general Protein Science Membrane Biology Life sciences Biochemistry Proteins Cell membranes Membranproteine (DE-588)4130026-9 gnd Labortechnik (DE-588)4123602-6 gnd |
subject_GND | (DE-588)4130026-9 (DE-588)4123602-6 |
title | Membrane Proteomics Methods and Protocols |
title_auth | Membrane Proteomics Methods and Protocols |
title_exact_search | Membrane Proteomics Methods and Protocols |
title_full | Membrane Proteomics Methods and Protocols edited by Matthew J. Peirce, Robin Wait |
title_fullStr | Membrane Proteomics Methods and Protocols edited by Matthew J. Peirce, Robin Wait |
title_full_unstemmed | Membrane Proteomics Methods and Protocols edited by Matthew J. Peirce, Robin Wait |
title_short | Membrane Proteomics |
title_sort | membrane proteomics methods and protocols |
title_sub | Methods and Protocols |
topic | Life Sciences Biochemistry, general Protein Science Membrane Biology Life sciences Biochemistry Proteins Cell membranes Membranproteine (DE-588)4130026-9 gnd Labortechnik (DE-588)4123602-6 gnd |
topic_facet | Life Sciences Biochemistry, general Protein Science Membrane Biology Life sciences Biochemistry Proteins Cell membranes Membranproteine Labortechnik |
url | https://doi.org/10.1007/978-1-60327-310-7 |
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