Microtubule Dynamics: Methods and Protocols
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field pr...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2011
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
777 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field |
| Beschreibung: | 1 Online-Ressource (XI, 319 p. 56 illus., 13 illus. in color) |
| ISBN: | 9781617792526 |
| DOI: | 10.1007/978-1-61779-252-6 |
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| spelling | Microtubule Dynamics Methods and Protocols edited by Anne Straube Totowa, NJ Humana Press 2011 1 Online-Ressource (XI, 319 p. 56 illus., 13 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 777 Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field Life Sciences Cell Biology Cell Physiology Life sciences Cell biology Cell physiology Mikrotubulus (DE-588)4169864-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Mikrotubulus (DE-588)4169864-2 s 2\p DE-604 Straube, Anne edt Erscheint auch als Druck-Ausgabe 9781617792519 https://doi.org/10.1007/978-1-61779-252-6 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Microtubule Dynamics Methods and Protocols Life Sciences Cell Biology Cell Physiology Life sciences Cell biology Cell physiology Mikrotubulus (DE-588)4169864-2 gnd |
| subject_GND | (DE-588)4169864-2 (DE-588)4143413-4 |
| title | Microtubule Dynamics Methods and Protocols |
| title_auth | Microtubule Dynamics Methods and Protocols |
| title_exact_search | Microtubule Dynamics Methods and Protocols |
| title_full | Microtubule Dynamics Methods and Protocols edited by Anne Straube |
| title_fullStr | Microtubule Dynamics Methods and Protocols edited by Anne Straube |
| title_full_unstemmed | Microtubule Dynamics Methods and Protocols edited by Anne Straube |
| title_short | Microtubule Dynamics |
| title_sort | microtubule dynamics methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Cell Physiology Life sciences Cell biology Cell physiology Mikrotubulus (DE-588)4169864-2 gnd |
| topic_facet | Life Sciences Cell Biology Cell Physiology Life sciences Cell biology Cell physiology Mikrotubulus Aufsatzsammlung |
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