Lipase and phospholipase protocols:
Lipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Schriftenreihe: | Methods in Molecular Biology™
109 |
| Schlagworte: | |
| Online-Zugang: | UBR01 URL des Erstveröffentlichers |
| Zusammenfassung: | Lipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for the determination of lipolytic activity, proven strategies for the expression and purification of recombinant lipases and phospholipases, and the characterization of lipases by such techniques as immunodetection, subunit size determination, lipase kinetics, and ligand interactions. In addition, there are methods for the molecular and genetic analysis of the lipases, the quantification of mRNA levels, and in vitro-coupled transcription/translation. The protocols cover more than a dozen specific enzymes, and many can be applied directly to all lipases and phospholipases. Lipase and Phospholipase Protocols constitutes a highly useful single-volume handbook of basic procedures and strategies to investigate this unique class of enzymes. Its step-by-step format works perfectly at the laboratory bench or by the computer, providing practical information and examples that will lead to successful design and execution of specific techniques. It not only furnishes a technical framework for the investigation of known lipases and phospholipases, but provides well-established methods readily daptable to the discovery of new lipase enzymes |
| Beschreibung: | 1 Online-Ressource (XVII, 362 p) |
| ISBN: | 9781592595815 |
| DOI: | 10.1385/1592595812 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044950427 | ||
| 003 | DE-604 | ||
| 005 | 20180827 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s1999 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592595815 |9 978-1-59259-581-5 | ||
| 024 | 7 | |a 10.1385/1592595812 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-581-5 | ||
| 035 | |a (OCoLC)1036354604 | ||
| 035 | |a (DE-599)BVBBV044950427 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 082 | 0 | |a 572 |2 23 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a WC 4350 |0 (DE-625)148095: |2 rvk | ||
| 084 | |a WD 5050 |0 (DE-625)148185: |2 rvk | ||
| 245 | 1 | 0 | |a Lipase and phospholipase protocols |c edited by Mark Doolittle, Karen Reue |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1999 | |
| 300 | |a 1 Online-Ressource (XVII, 362 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology™ |v 109 | |
| 520 | |a Lipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for the determination of lipolytic activity, proven strategies for the expression and purification of recombinant lipases and phospholipases, and the characterization of lipases by such techniques as immunodetection, subunit size determination, lipase kinetics, and ligand interactions. In addition, there are methods for the molecular and genetic analysis of the lipases, the quantification of mRNA levels, and in vitro-coupled transcription/translation. The protocols cover more than a dozen specific enzymes, and many can be applied directly to all lipases and phospholipases. Lipase and Phospholipase Protocols constitutes a highly useful single-volume handbook of basic procedures and strategies to investigate this unique class of enzymes. Its step-by-step format works perfectly at the laboratory bench or by the computer, providing practical information and examples that will lead to successful design and execution of specific techniques. It not only furnishes a technical framework for the investigation of known lipases and phospholipases, but provides well-established methods readily daptable to the discovery of new lipase enzymes | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Biochemistry, general | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Biochemistry | |
| 650 | 0 | 7 | |a Lipasen |0 (DE-588)4167788-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biochemische Analyse |0 (DE-588)4255721-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Phospholipasen |0 (DE-588)4174399-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Lipasen |0 (DE-588)4167788-2 |D s |
| 689 | 0 | 1 | |a Biochemische Analyse |0 (DE-588)4255721-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Phospholipasen |0 (DE-588)4174399-4 |D s |
| 689 | 1 | 1 | |a Biochemische Analyse |0 (DE-588)4255721-5 |D s |
| 689 | 1 | |5 DE-604 | |
| 700 | 1 | |a Doolittle, Mark |4 edt | |
| 700 | 1 | |a Reue, Karen |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 978-0-89603-546-1 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/1592595812 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030343188 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1385/1592595812 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178537842737152 |
|---|---|
| any_adam_object | |
| author2 | Doolittle, Mark Reue, Karen |
| author2_role | edt edt |
| author2_variant | m d md k r kr |
| author_facet | Doolittle, Mark Reue, Karen |
| building | Verbundindex |
| bvnumber | BV044950427 |
| classification_rvk | WC 4150 WC 4350 WD 5050 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-581-5 (OCoLC)1036354604 (DE-599)BVBBV044950427 |
| dewey-full | 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572 |
| dewey-search | 572 |
| dewey-sort | 3572 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1385/1592595812 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03555nmm a2200589zcb4500</leader><controlfield tag="001">BV044950427</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180827 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s1999 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592595815</subfield><subfield code="9">978-1-59259-581-5</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/1592595812</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-581-5</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1036354604</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044950427</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4350</subfield><subfield code="0">(DE-625)148095:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5050</subfield><subfield code="0">(DE-625)148185:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Lipase and phospholipase protocols</subfield><subfield code="c">edited by Mark Doolittle, Karen Reue</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XVII, 362 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology™</subfield><subfield code="v">109</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Lipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for the determination of lipolytic activity, proven strategies for the expression and purification of recombinant lipases and phospholipases, and the characterization of lipases by such techniques as immunodetection, subunit size determination, lipase kinetics, and ligand interactions. In addition, there are methods for the molecular and genetic analysis of the lipases, the quantification of mRNA levels, and in vitro-coupled transcription/translation. The protocols cover more than a dozen specific enzymes, and many can be applied directly to all lipases and phospholipases. Lipase and Phospholipase Protocols constitutes a highly useful single-volume handbook of basic procedures and strategies to investigate this unique class of enzymes. Its step-by-step format works perfectly at the laboratory bench or by the computer, providing practical information and examples that will lead to successful design and execution of specific techniques. It not only furnishes a technical framework for the investigation of known lipases and phospholipases, but provides well-established methods readily daptable to the discovery of new lipase enzymes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry, general</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Lipasen</subfield><subfield code="0">(DE-588)4167788-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biochemische Analyse</subfield><subfield code="0">(DE-588)4255721-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Phospholipasen</subfield><subfield code="0">(DE-588)4174399-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Lipasen</subfield><subfield code="0">(DE-588)4167788-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Biochemische Analyse</subfield><subfield code="0">(DE-588)4255721-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Phospholipasen</subfield><subfield code="0">(DE-588)4174399-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Biochemische Analyse</subfield><subfield code="0">(DE-588)4255721-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Doolittle, Mark</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Reue, Karen</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">978-0-89603-546-1</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/1592595812</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343188</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1592595812</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044950427 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:33Z |
| institution | BVB |
| isbn | 9781592595815 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343188 |
| oclc_num | 1036354604 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 1 Online-Ressource (XVII, 362 p) |
| psigel | ZDB-2-PRO |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology™ |
| spelling | Lipase and phospholipase protocols edited by Mark Doolittle, Karen Reue Totowa, NJ Humana Press 1999 1 Online-Ressource (XVII, 362 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 109 Lipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for the determination of lipolytic activity, proven strategies for the expression and purification of recombinant lipases and phospholipases, and the characterization of lipases by such techniques as immunodetection, subunit size determination, lipase kinetics, and ligand interactions. In addition, there are methods for the molecular and genetic analysis of the lipases, the quantification of mRNA levels, and in vitro-coupled transcription/translation. The protocols cover more than a dozen specific enzymes, and many can be applied directly to all lipases and phospholipases. Lipase and Phospholipase Protocols constitutes a highly useful single-volume handbook of basic procedures and strategies to investigate this unique class of enzymes. Its step-by-step format works perfectly at the laboratory bench or by the computer, providing practical information and examples that will lead to successful design and execution of specific techniques. It not only furnishes a technical framework for the investigation of known lipases and phospholipases, but provides well-established methods readily daptable to the discovery of new lipase enzymes Life Sciences Biochemistry, general Life sciences Biochemistry Lipasen (DE-588)4167788-2 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Phospholipasen (DE-588)4174399-4 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Lipasen (DE-588)4167788-2 s Biochemische Analyse (DE-588)4255721-5 s DE-604 Phospholipasen (DE-588)4174399-4 s Doolittle, Mark edt Reue, Karen edt Erscheint auch als Druck-Ausgabe 978-0-89603-546-1 https://doi.org/10.1385/1592595812 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Lipase and phospholipase protocols Life Sciences Biochemistry, general Life sciences Biochemistry Lipasen (DE-588)4167788-2 gnd Biochemische Analyse (DE-588)4255721-5 gnd Phospholipasen (DE-588)4174399-4 gnd |
| subject_GND | (DE-588)4167788-2 (DE-588)4255721-5 (DE-588)4174399-4 (DE-588)4143413-4 |
| title | Lipase and phospholipase protocols |
| title_auth | Lipase and phospholipase protocols |
| title_exact_search | Lipase and phospholipase protocols |
| title_full | Lipase and phospholipase protocols edited by Mark Doolittle, Karen Reue |
| title_fullStr | Lipase and phospholipase protocols edited by Mark Doolittle, Karen Reue |
| title_full_unstemmed | Lipase and phospholipase protocols edited by Mark Doolittle, Karen Reue |
| title_short | Lipase and phospholipase protocols |
| title_sort | lipase and phospholipase protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Lipasen (DE-588)4167788-2 gnd Biochemische Analyse (DE-588)4255721-5 gnd Phospholipasen (DE-588)4174399-4 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Lipasen Biochemische Analyse Phospholipasen Aufsatzsammlung |
| url | https://doi.org/10.1385/1592595812 |
| work_keys_str_mv | AT doolittlemark lipaseandphospholipaseprotocols AT reuekaren lipaseandphospholipaseprotocols |