Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols
Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into t...
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| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2013
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
969 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming |
| Beschreibung: | 1 Online-Ressource (XI, 324 p. 49 illus., 18 illus. in color) |
| ISBN: | 9781627032605 |
| DOI: | 10.1007/978-1-62703-260-5 |
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| 520 | |a Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming | ||
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| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
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| spelling | Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols edited by Peter M. Rabinovich Totowa, NJ Humana Press 2013 1 Online-Ressource (XI, 324 p. 49 illus., 18 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 969 Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming Biomedicine Human Genetics Medicine Human genetics Rabinovich, Peter M. edt Erscheint auch als Druck-Ausgabe 9781627032599 https://doi.org/10.1007/978-1-62703-260-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols Biomedicine Human Genetics Medicine Human genetics |
| title | Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols |
| title_auth | Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols |
| title_exact_search | Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols |
| title_full | Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols edited by Peter M. Rabinovich |
| title_fullStr | Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols edited by Peter M. Rabinovich |
| title_full_unstemmed | Synthetic Messenger RNA and Cell Metabolism Modulation Methods and Protocols edited by Peter M. Rabinovich |
| title_short | Synthetic Messenger RNA and Cell Metabolism Modulation |
| title_sort | synthetic messenger rna and cell metabolism modulation methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics |
| url | https://doi.org/10.1007/978-1-62703-260-5 |
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