Cytokine Bioassays: Methods and Protocols
Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the fir...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2014
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| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1172 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research |
| Beschreibung: | 1 Online-Ressource (XIV, 367 p. 87 illus., 74 illus. in color) |
| ISBN: | 9781493909285 |
| DOI: | 10.1007/978-1-4939-0928-5 |
Internformat
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| 520 | |a Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research | ||
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| dewey-hundreds | 600 - Technology (Applied sciences) |
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| spelling | Cytokine Bioassays Methods and Protocols edited by Ivana Vancurova New York, NY Springer New York 2014 1 Online-Ressource (XIV, 367 p. 87 illus., 74 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1172 Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research Biomedicine Immunology Cytokines and Growth Factors Medicine Cytokines Growth factors Bioassay (DE-588)4201084-6 gnd rswk-swf Cytokine (DE-588)4205701-2 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Cytokine (DE-588)4205701-2 s Bioassay (DE-588)4201084-6 s Labortechnik (DE-588)4123602-6 s b DE-604 Vancurova, Ivana edt Erscheint auch als Druck-Ausgabe 9781493909278 https://doi.org/10.1007/978-1-4939-0928-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Cytokine Bioassays Methods and Protocols Biomedicine Immunology Cytokines and Growth Factors Medicine Cytokines Growth factors Bioassay (DE-588)4201084-6 gnd Cytokine (DE-588)4205701-2 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4201084-6 (DE-588)4205701-2 (DE-588)4123602-6 |
| title | Cytokine Bioassays Methods and Protocols |
| title_auth | Cytokine Bioassays Methods and Protocols |
| title_exact_search | Cytokine Bioassays Methods and Protocols |
| title_full | Cytokine Bioassays Methods and Protocols edited by Ivana Vancurova |
| title_fullStr | Cytokine Bioassays Methods and Protocols edited by Ivana Vancurova |
| title_full_unstemmed | Cytokine Bioassays Methods and Protocols edited by Ivana Vancurova |
| title_short | Cytokine Bioassays |
| title_sort | cytokine bioassays methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Immunology Cytokines and Growth Factors Medicine Cytokines Growth factors Bioassay (DE-588)4201084-6 gnd Cytokine (DE-588)4205701-2 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Biomedicine Immunology Cytokines and Growth Factors Medicine Cytokines Growth factors Bioassay Cytokine Labortechnik |
| url | https://doi.org/10.1007/978-1-4939-0928-5 |
| work_keys_str_mv | AT vancurovaivana cytokinebioassaysmethodsandprotocols |