Adipose Tissue Protocols:
Adipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries. In Adipose Tissue Protocols, Gérard Ailhaud and a team of laborato...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Springer New York
2001
|
| Schriftenreihe: | Methods in Molecular Biology™
155 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Adipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries. In Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded the isolation, subcellular fractionation, and transfection of low density adipocytes, as well as the metabolic aspects of nutrient uptake and key assays of nutrient and ion fluxes. Also covered are: biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT; measurements of adipose secretory products; assessment of WAT metabolism in vivo; and assays of lipid-related enzymes. Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective nutritional, physiological, and molecular-level research on adipose tissue |
| Beschreibung: | 1 Online-Ressource (XIII, 334 p) |
| ISBN: | 9781592592319 |
| DOI: | 10.1385/1592592317 |
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| 520 | |a Adipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries. In Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded the isolation, subcellular fractionation, and transfection of low density adipocytes, as well as the metabolic aspects of nutrient uptake and key assays of nutrient and ion fluxes. Also covered are: biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT; measurements of adipose secretory products; assessment of WAT metabolism in vivo; and assays of lipid-related enzymes. Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective nutritional, physiological, and molecular-level research on adipose tissue | ||
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| doi_str_mv | 10.1385/1592592317 |
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| spelling | Adipose Tissue Protocols edited by Gérard Ailhaud Totowa, NJ Springer New York 2001 1 Online-Ressource (XIII, 334 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 155 Adipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries. In Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded the isolation, subcellular fractionation, and transfection of low density adipocytes, as well as the metabolic aspects of nutrient uptake and key assays of nutrient and ion fluxes. Also covered are: biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT; measurements of adipose secretory products; assessment of WAT metabolism in vivo; and assays of lipid-related enzymes. Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective nutritional, physiological, and molecular-level research on adipose tissue Life Sciences Cell Biology Life sciences Cell biology Biochemische Methode (DE-588)4319880-6 gnd rswk-swf Fettgewebe (DE-588)4154224-1 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Endokrine Regulation (DE-588)4113434-5 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Fettgewebe (DE-588)4154224-1 s Labortechnik (DE-588)4123602-6 s DE-604 Zellkultur (DE-588)4067547-6 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s Endokrine Regulation (DE-588)4113434-5 s Biochemische Methode (DE-588)4319880-6 s Ailhaud, Gérard edt Erscheint auch als Druck-Ausgabe 9780896037472 https://doi.org/10.1385/1592592317 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Adipose Tissue Protocols Life Sciences Cell Biology Life sciences Cell biology Biochemische Methode (DE-588)4319880-6 gnd Fettgewebe (DE-588)4154224-1 gnd Zellkultur (DE-588)4067547-6 gnd Labortechnik (DE-588)4123602-6 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd Endokrine Regulation (DE-588)4113434-5 gnd |
| subject_GND | (DE-588)4319880-6 (DE-588)4154224-1 (DE-588)4067547-6 (DE-588)4123602-6 (DE-588)4038971-6 (DE-588)4039983-7 (DE-588)4113434-5 (DE-588)4143413-4 |
| title | Adipose Tissue Protocols |
| title_auth | Adipose Tissue Protocols |
| title_exact_search | Adipose Tissue Protocols |
| title_full | Adipose Tissue Protocols edited by Gérard Ailhaud |
| title_fullStr | Adipose Tissue Protocols edited by Gérard Ailhaud |
| title_full_unstemmed | Adipose Tissue Protocols edited by Gérard Ailhaud |
| title_short | Adipose Tissue Protocols |
| title_sort | adipose tissue protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Biochemische Methode (DE-588)4319880-6 gnd Fettgewebe (DE-588)4154224-1 gnd Zellkultur (DE-588)4067547-6 gnd Labortechnik (DE-588)4123602-6 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd Endokrine Regulation (DE-588)4113434-5 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Biochemische Methode Fettgewebe Zellkultur Labortechnik Methode Molekularbiologie Endokrine Regulation Aufsatzsammlung |
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