Morte à morte!: 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | Portuguese |
| Veröffentlicht: |
Lisboa
Assembleia da República
2017
|
| Schriftenreihe: | Imagens e documentos
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | Publ. on the occasion of an exhibition held at the Assembleia da República, Lisbon, Portugal, July 5, 2017-February 1, 2018 |
| Beschreibung: | 141 Seiten |
| ISBN: | 9789725566848 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804178535931183104 |
|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS
ABSTRACT I
1 INTRODUCTION 1
1.1 BRYOPHYTES: NON-VASCULAR LAND PLANTS WITH A UNIQUE ECOPHYSIOLOGY 1
1.2 THE MOSS PHYSCOMITRETTA PATENS AS A MODEL ORGANISM 4
1.2.1 THE HAPLOID-DOMINANT LIFE CYCLE 5
1.2.2 ECOLOGY AND COLD STRESS TOLERANCE 7
1.2.3 POLYUNSATURATED FATTY ACIDS AND OXYLIPINS 9
1.2.4 THE GENOME 11
1.3 COLD STRESS 12
1.3.1 TRANSCRIPTOMIC REGULONS IN VASCULAR PLANTS 15
1.3.2 INSIGHTS INTO THE COLD STRESS RESPONSE OF PHYSCOMITRELLA PATENS 16
1.4 OXIDATIVE STRESS AND PHOTOPROTECTIVE MECHANISMS 17
1.5 AIM OF THIS WORK 20
2 MATERIAL AND METHODS 2 1
2.1 CHEMICALS 2 1
2.2 MEDIA 2 1
2.3 BUFFERS AND SOLUTIONS 22
2.4 CELL CULTURE TECHNIQUES 27
2.4.1 PLANT MATERIAL AND AXENIC IN VITRO CULTIVATION 27
2.4.2 COLD TREATMENTS 27
2.4.3 FREEZING EXPERIMENTS 29
2.4.4 BIOMASS MEASUREMENT 29
2.4.5 SPOROPHYTE INDUCTION 29
2.4.6 CRYOCONSERVATION 29
2.5 PHENOTYPIC ANALYSIS 30
2.6 IN VIVO CHLOROPHYLL FLUORESCENCE MEASUREMENT 30
2.7 GENERATION OF TRANSGENIC MOSS LINES 3 1
2.7.1 PROTOPLAST ISOLATION 3 1
2.7.2 TRANSFORMATION OF PROTOPLASTS 31
2.7.3 SELECTION OF TRANSFORMANTS 32
2.8 MOLECULAR BIOLOGY 33
2.8.1 EXTRACTION OF GENOMIC DNA 33
2.8.2 TOTAL RNA EXTRACTION 34
2.8.3 CDNA SYNTHESIS 35
2.8.4 POLYMERASE CHAIN REACTION 36
2.8.5 AGAROSE GEL ELECTROPHORESIS 38
2.8.6 SEQUENCING OF NUCLEIC ACIDS 38
2.8.7 MOLECULAR CLONING OF DNA FRAGMENTS 39
2.8.8 TRANSFORMATION OF CHEMICALLY COMPETENT E. COLI CELLS 4 1
HTTP://D-NB.INFO/1036370992
IMAGE 2
2.8.9. PLASMID DNA EXTRACTION 4 1
2.8.10 SOUTHERN BLOT 4 1
2.8.11 QUANTITATIVE REAL-TIME PCR 43
2.9 MICROARRAY HYBRIDIZATION 4 3
2.9.1 CHIP DESIGN AND EXPERIMENTAL SETUP 43
2.9.2 AMPLIFIED RNA SYNTHESIS AND LABELLING 44
2.9.3 FRAGMENTATION OF ARNA 44
2.9.4 HYBRIDIZATION OF THE MICROARRAYS 44
2.9.5 WASHING AND STRIPPING OF THE MICROARRAYS 45
2.10 ANALYTICAL BIOLOGY 47
2.10.1 CAROTENOID EXTRACTION AND ANALYSIS 47
2.10.2 FATTY ACID EXTRACTION AND ANALYSIS 47
2.11 COMPUTATIONAL BIOLOGY 49
2.11.1 PRIMER DESIGN 49
2.11.2 SEQUENCE ANALYSIS 52
2.11.3 MULTIPLE SEQUENCE ALIGNMENTS AND PHYLOGENIES 53
2.11.4 ESTABLISHMENT OF MOLECULAR CLONING STRATEGIES AND PLASMID MAPS 53
2.11.5 LARGE SCALE EXPRESSION PROFILING 53
2.11.6. STATISTICAL ANALYSIS AND DEPICTION OF EXPERIMENTAL DATA 57
3 RESULTS 59
3.1. TEMPERATURE REDUCTION CAUSES GROWTH INHIBITION, DEHYDRATION AND
BLEACHING 59 3.2 ASSESSING THE FREEZING TOLERANCE OF GAMETOPHORES 62
3.3 ANALYSIS AND VALIDATION OF THE MICROARRAY EXPERIMENTS 63
3.3.1 RAW DATA VISUALIZATION AND NORMALIZATION 63
3.3.2 DIFFERENTIAL GENE EXPRESSION 65
3.3.3 VALIDATION OF GENE EXPRESSION PATTERNS 70
3.4 TIME-SERIES ANALYSIS OF THE LARGE-SCALE EXPRESSION DATA 72
3.4.1 PRINCIPAL COMPONENT ANALYSIS 72
3.4.2 GENE ONTOLOGY ANNOTATION OF DIFFERENTIALLY EXPRESSED GENES 73
3.4.3 EARLY DIFFERENTIALLY EXPRESSED GENES 73
3.4.4 INTERMEDIATE DIFFERENTIALLY EXPRESSED GENES 77
3.4.5 LATE DIFFERENTIALLY EXPRESSED GENES 82
3.4.6 SELECTED ASPECTS OF THE TIME-SERIES ANALYSIS 86
3.5 DIFFERENTIALLY EXPRESSION OF CALCIUM SENSOR-CODING GENES 87
3.6 DIFFERENTIALLY EXPRESSED TRANSCRIPTION FACTOR-CODING GENES 88
3.6.1 CROSS-TALK AND SPECIFICITY 96
3.6.2 ABA-DEPENDENT TRANSCRIPTION FACTOR EXPRESSION 97
3.7 METABOLIC PATHWAY MAPPING OF THE DIFFERENTIALLY EXPRESSED GENES 100
3.8 ABA-DEPENDENT GENE EXPRESSION 103
3.9 SYNTHESIS OF CELL- AND OSMOPROTECTIVE SUBSTANCES 104
3.9.1 AMINO ACID METABOLISM: POLYAMINE BIOSYNTHESIS 104
3.9.2 CARBOHYDRATE METABOLISM: STARCH DEGRADATION AND SUCROSE SYNTHESIS
106 3.10 PHOTOINHIBITION AND PHOTOPROTECTION 108
3.10.1 EXPRESSION PATTERNS OF GENES INVOLVED IN PHOTOSYNTHESIS 108
IMAGE 3
3.10.2 CAROTENOID PROFILES INDICATE XANTHOPHYLL-DEPENDENT
PHOTOPROTECTION 114
3.10.3 IN VIVO CHLOROPHYLL FLUORESCENCE MEASUREMENT INDICATES
PHOTOINHIBITION AND INCREASED NON-PHOTOCHEMICAL QUENCHING 116
3.11 CHARACTERIZATION OF A COLD-INDUCED PUTATIVE FATTY ACID DESATURASE
117
3.11.1 COMPARATIVE FATTY ACID PROFILES OF SIX DIFFERENT MOSS SPECIES 117
3.11.2 CHANGES IN THE FATTY ACID METABOLISM IN RESPONSE TO COLD STRESS
119
3.11.3 DIFFERENTIAL EXPRESSION OF FATTY ACID DESATURASE-CODING GENES 120
3.11.4 SEQUENCE ANALYSIS AND PHYLOGENY OF THE A6-FADS-LIKE-FAMILY 121
3.11.5 SUBCELLULAR LOCALIZATION OF THE COLD-INDUCED PUTATIVE FATTY ACID
DESATURASE 125 3.11.6 EXPRESSION PATTERNS IN RESPONSE TO ABIOTIC AND
BIOTIC STRESS 126
3.11.7 KNOCKOUT VALIDATION 128
3.11.8 PHENOTYPIC AND BIOCHEMICAL CHARACTERIZATION 131
3.11.9 TRANSCRIPTIONAL EVIDENCE FOR SPHINGOLIPID SYNTHESIS DURING COLD
STRESS 135 3.12 COMPARISON WITH LARGE-SCALE EXPRESSION DATA FROM
ARABIDOPSIS THALIANA 136 3.13 DIFFERENTIAL EXPRESSION OF POTENTIALLY
MOSS-SPECIFIC GENES DURING COLD STRESS 141 3.13.1 A GENE OF UNKNOWN
FUNCTION IS THE MOST HIGHLY INDUCED GENE DURING EARLY COLD STRESS
RESPONSE 145
3.13.2 SEQUENCE ANALYSIS OF THE GENE OF UNKNOWN FUNCTION FROM DIFFERENT
FUNARIACEAE 145
4 DISCUSSION 149
4.1 PHYSCOMITRELLA PATENS AS A MODEL ORGANISM FOR STUDYING COLD
ACCLIMATION OF NON-VASCULAR LAND PLANTS 149
4.2 THE TRANSCRIPTOMIC AND PHYSIOLOGICAL COLD STRESS RESPONSE OF
PHYSCOMITRELLA PATENS 151 4.3 THE PHYSCOMITRELLA COLD STRESS
TRANSCRIPTOME PROVIDES INSIGHTS INTO THE EVOLUTION OF COLDACCLIMATORY
MECHANISMS IN LAND PLANTS 163
4.4 LIPIDOMICS AND COLD STRESS SIGNALLING IN PHYSCOMITRELLA PATENS 168
5 PUBLICATIONS AND CONFERENCE CONTRIBUTIONS 173
5.1 PUBLICATIONS 173
5.2 TALKS 174
5.3 POSTERS 174
6 LIST OF ABBREVIATIONS 175
7 REFERENCES , 177
8 APPENDIX 189
8.1 SEQUENCES 189
8.1.1 THE PUTATIVE FATTY ACID DESATURASE 189
8.1.2 THE GENE OF UNKNOWN FUNCTION 189
DANKSAGUNG 191
ERKLARUNG 193
INTRODUCAO
-
CONTRA
A
LEI
DE
MATAR
PORTUGAL,
UM
PAIS
PIONEIRO
NA
ABOLICAO
DA
PENA
DE
MORTE
A
LEI
DE
TALIAO
ABOLICIONISMO
-
INDICADORES
DE
CIVILIZACAO
-
UM
INSTRUMENTO
DE
PACIFICACAO
POLITICA
NA
IMPLANTACAO
DO
LIBERALISMO
ABOLICAO
-
DOS
PRINCIPIOS
A
LEI
UM
CORRECIONALISMO
MAL
ASSIMILADO
0
ABOLICIONISMO
NA
I
REPUBLICA
O
RETORNO
DA
FORCA
E
DO
CARRASCO
-
PODIA
A
REPUBLICA
REINSTAURAR
A
PENA
CAPITAL
QUE
A
MONARQUIA
ABOLIU?
ABOLICAO
DA
PENA
DE
MORTE
-
UMA
MEMORIA
PARTILHADA
A
PENA
DE
MORTE
EM
PORTUGAL
E
NO
MUNDO
-
OLHARES
CRUZADOS
PENA
DE
MORTE
-
UM
TEMA
PARA
OS
DIAS
DE
HOJE
CRONOLOGIA
FONTES
E
BIBLIOGRAFIA
INTRODUJO - CONTRA A LEI DE MATAR
PORTUGAL, UM PAÍS PIONEIRO NA ABOLI^ÁO DA PENA DE MORTE
A LEI DE TALIÁO
INDICADORES DE CIVILIZADO
ABOLICIONISMO - UM INSTRUMENTO DE PACIFICADO POLÍTICA
NA IMPLANTADO DO LIBERALISMO
ABOLIDO D0S PRINCÍPIOS Á LEI
UM CORRECIONALISMO MAL ASSIMILADO
O ABOLICIONISMO NA I REPÚBLICA
O RETORNO DA FORCA E DO CARRASCO - PODIA A REPÚBLICA
REINSTAURAR A PENA CAPITAL QUE A MONARQUIA ABOLIU?
ABOLIDO DA PENA DE MORTE - UMA MEMORIA PARTILHADA
A PENA DE MORTE EM PORTUGAL E NO MUNDO - OLHARES CRUZADOS
PENA DE MORTE - UM TEMA PARA OS DIAS DE HOJE
CRONOLOGIA
FONTES E BIBLIOGRAFIA
INTRODUJO - CONTRA A LEI DE MATAR
PORTUGAL, UM PAÍS PIONEIRO NA ABOLI^ÁO DA PENA DE MORTE
A LEI DE TALIÁO
INDICADORES DE CIVILIZADO
ABOLICIONISMO - UM INSTRUMENTO DE PACIFICADO POLÍTICA
NA IMPLANTADO DO LIBERALISMO
ABOLIDO D0S PRINCÍPIOS Á LEI
UM CORRECIONALISMO MAL ASSIMILADO
O ABOLICIONISMO NA I REPÚBLICA
O RETORNO DA FORCA E DO CARRASCO - PODIA A REPÚBLICA
REINSTAURAR A PENA CAPITAL QUE A MONARQUIA ABOLIU?
ABOLIDO DA PENA DE MORTE - UMA MEMORIA PARTILHADA
A PENA DE MORTE EM PORTUGAL E NO MUNDO - OLHARES CRUZADOS
PENA DE MORTE - UM TEMA PARA OS DIAS DE HOJE
CRONOLOGIA
FONTES E BIBLIOGRAFIA
|
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| indexdate | 2024-07-10T08:05:31Z |
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| oclc_num | 1036370992 |
| open_access_boolean | |
| owner | DE-12 |
| owner_facet | DE-12 |
| physical | 141 Seiten |
| publishDate | 2017 |
| publishDateSearch | 2017 |
| publishDateSort | 2017 |
| publisher | Assembleia da República |
| record_format | marc |
| series2 | Imagens e documentos |
| spelling | Morte à morte! 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 coordenação Luís Farinha Lisboa Assembleia da República 2017 141 Seiten txt rdacontent n rdamedia nc rdacarrier Imagens e documentos Publ. on the occasion of an exhibition held at the Assembleia da República, Lisbon, Portugal, July 5, 2017-February 1, 2018 Geschichte 1867-2017 gnd rswk-swf Justiz (DE-588)4073136-4 gnd rswk-swf Abschaffung (DE-588)4200586-3 gnd rswk-swf Todesstrafe (DE-588)4060306-4 gnd rswk-swf Portugal (DE-588)4046843-4 gnd rswk-swf (DE-588)4135467-9 Ausstellungskatalog gnd-content Portugal (DE-588)4046843-4 g Justiz (DE-588)4073136-4 s Todesstrafe (DE-588)4060306-4 s Abschaffung (DE-588)4200586-3 s Geschichte 1867-2017 z DE-604 Farinha, Luís Manuel 1954- (DE-588)1074920163 edt DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030340109&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung BSB Muenchen - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030340109&sequence=000005&line_number=0003&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung BSB Muenchen - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030340109&sequence=000007&line_number=0004&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Morte à morte! 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 Justiz (DE-588)4073136-4 gnd Abschaffung (DE-588)4200586-3 gnd Todesstrafe (DE-588)4060306-4 gnd |
| subject_GND | (DE-588)4073136-4 (DE-588)4200586-3 (DE-588)4060306-4 (DE-588)4046843-4 (DE-588)4135467-9 |
| title | Morte à morte! 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 |
| title_auth | Morte à morte! 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 |
| title_exact_search | Morte à morte! 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 |
| title_full | Morte à morte! 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 coordenação Luís Farinha |
| title_fullStr | Morte à morte! 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 coordenação Luís Farinha |
| title_full_unstemmed | Morte à morte! 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 coordenação Luís Farinha |
| title_short | Morte à morte! |
| title_sort | morte a morte 150 anos de abolicao da pena de morte em portugal 1867 2017 exposicao 6 de julho a 29 de dezembro de 2017 |
| title_sub | 150 anos de abolição da pena de morte em Portugal, 1867-2017 : exposição 6 de julho a 29 de dezembro de 2017 |
| topic | Justiz (DE-588)4073136-4 gnd Abschaffung (DE-588)4200586-3 gnd Todesstrafe (DE-588)4060306-4 gnd |
| topic_facet | Justiz Abschaffung Todesstrafe Portugal Ausstellungskatalog |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030340109&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030340109&sequence=000005&line_number=0003&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030340109&sequence=000007&line_number=0004&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT farinhaluismanuel morteamorte150anosdeabolicaodapenademorteemportugal18672017exposicao6dejulhoa29dedezembrode2017 |
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