UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
Gießen, Lahn
VVB Laufersweiler Verlag
2017
|
| Schriftenreihe: | Edition Scientifique
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| ISBN: | 9783835965874 3835965875 |
Internformat
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| 245 | 1 | 0 | |a UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN |c Elisa-Marie Laux |
| 263 | |a 201707 | ||
| 264 | 1 | |a Gießen, Lahn |b VVB Laufersweiler Verlag |c 2017 | |
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Datensatz im Suchindex
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| adam_text | INHALTSVERZEICHNIS
1
EINLEITUNG.............................................................................................................
1
2
LITERATUR................................................................................................................
2
2.1 LICHTMIKROSKOPISCHE FAERBEMETHODEN FUER RUEDENSPERMA
..................................
2
2.1.1
PAPANICOLAOU-SHORR..............................................................................................2
2.1.2
EOSIN......................................................................................................................2
2.1.3
DIFF-QUICK..............................................................................................................3
2.1.4 TRYPANBLAU UND
GIEMSA.......................................................................................4
2.1.5
SPERMAC................................................................................................................
4
2.1.6
TRIPLE-STAIN............................................................................................................4
2.1.7
FORMOLCITRAT-FLUESSIGFIXIERUNG...............................................................................
5
2.1.8
TOLUIDINBLAU...........................................................................................................5
2.1.9
ANILINBLAU...............................................................................................................
6
2.1.10
SPERMIENCHROMATINDISPERSIONSTEST.....................................................................7
2.2 FL UORESZE NS MIKROSKOPISCHE FAERBEMETHODEN FUER
RUEDENSPERMA.......................8
2.2.1 PROPIDIUMIODID / ETHIDI UM HOMODIMER
.................................................................
6
2.2.2 CARBOXYFLUORESCEIN UND
KOMBINATIONEN.............................................................9
2.2.3 HOECHST
33258.....................................................................................................
10
2.2.4 SYBR-14 UND
KOMBINATIONEN............................................................................
10
2.2.5 RHODAMINE
123...................................................................................................11
2.2.6 5,5,6,6*-TETRACHLORO-1,1
*,3,3*-TETRAETHYLBENZIMIDAZOL-CARBOCYANINE IODIDE
(JC-1)
12
2.2.7 AKRIDINORANGE
12
2.2.8 TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE-MEDIATED DEOXYURIDINE
TRIPHATE-NICK
END LABELING ASSAY
(TUNEL)........................................................................................
14
2.2.9 ANNEXIN V
...........................................................................................................15
2.2.10 YO-PRO 1
..........................................................................................................15
2.2.11
LEKTINE...............................................................................................................
16
2.2.12
CHLORTETRAZYKLIN..................................................................................................16
2.2.13
MEROCYANIN-450.................................................................................................18
2.2.14 CHROMOMYCIN
A3................................................................................................16
3 MATERIAL UND
METHODEN...................................................................................
21
3.1
PROBANDEN.........................................................................................................
21
3.2 PROBENGEWINNUNG UND
AUFBEREITUNG................................................................21
3.2.1
SAMENENTNAHME...............................................................................................
21
3.2.2 ANFERTIGUNG VON SPRUEHFIXIERTEN
AUSSTRICHPRAEPARATEN.......................................22
3.3 SAMENUNTERSUCHUNG
........................................................................................
22
3.3.1 MAKROSKOPISCHE UNTERSUCHUNG
.......................................................................
22
3.3.2 COMPUTERASSISTIERTE SAMENANALYSE (CASA)
..................................................
23
3.3.2.1
AUFBAU................................................................................................................
23
3.3.2.2
ABLAUF.................................................................................................................
23
3.3.2.3 UNTERSUCHUNGSPARAMETER
................................................................................
24
3.3.3 MIKROSKOPISCHE FAERBEMETHODEN
......................................................................
26
3.3.3.1 SPERMIENVITALITAET UND PATHOMORPHOLOGIE
(EOSIN-FAERBUNG).............................26
3.3.3.2 SPERMIENREIFUNG (ANILINBLAUFAERBUNG)
26
3.3.3 3 CHROMATINDISSOZIATION (TOLUIDINBLAUFAERBUNG)
27
3.3.3 4 CHROMATINKONDENSATION (CHROMOMYCIN A3-FAERBUNG)
...................................
28
3.3.3 5 DNA-INTEGRITAET
(AKRIDINORANGE-TEST)................................................................
29
3.3.3 6 MODIFIZIERTER FLUORESZENZMIKROSKOPISCHER
SPERMIENCHROMATIN-STRUKTURASSAY
(MFSCSA).........................................................................................................................
30
3.3.3 7 DNA-FRAGMENTIERUNG (TUNEL-TEST)
...............................................................
31
3.3.3 6 APOPTOSENACHWEIS
(CASPASE-3-AKTIVITAET).......................................................33
3.4 ERFASSEN VON PRAKTIKABILITAETSKRITERIEN FUER DIE VETERINAERMEDIZINISCHE
PRAXIS.. 34
3.4.1 ERFASSEN DER ZEITDAUER
.....................................................................................34
3.4.2 BERECHNUNG DER MATERIALKOSTEN
........................................................................
35
3.4.3 BEWERTUNG DER VERWENDETEN SUBSTANZEN BEZUEGLICH GESUNDHEITS- UND
UMWELTBELASTUNG.............................................................................................................35
3.5 STATISTISCHE
AUSWERTUNG.....................................................................................35
4
ERGEBNISSE.......................................................................................................
38
4.1 MAKROSKOPISCHE UNTERSUCHUNG
........................................................................
36
4.2
MOTILITAET................................................................................................................
36
4.3
SPERMIENZAHL......................................................................................................
36
4.4
EOSINFAERBUNG......................................................................................................40
4.5
ANILINBLAUFAERBUNG...............................................................................................42
4.5.1 PROBLEMATIK DER ANILINBLAUFAERBUNG
..................................................................
42
4.5.2 FINANZIELLER UND ZEITLICHER AUFWAND DER
ANILINBLAUFAERBUNG.............................42
4.5.3 AUSWERTUNG DER
ANILINBLAUFAERBUNG....................................................................43
4.6 TOLUIDINBLAUFAERBUNG .45
4.6.1 PROBLEMATIK DER TOLUIDINBLAUFAERBUNG
..............................................................
4.6.2 FINANZIELLER UND ZEITLICHER AUFWAND DER TOLUIDINBLAUFAERBUNG
..........................
46
4.6.3 AUSWERTUNG DER
TOLUIDINBLAUFAERBUNG................................................................46
4.7.
CHROMOMYCINA3-FAERBUNG..................................................................................
48
4.7.1 PROBLEMATIK DER CMA3-FAERBUNG
......................................................................
48
4.7.2 FINANZIELLER UND ZEITLICHER AUFWAND DER CMA3-FAERBUNG
................................
49
4.7.3 AUSWERTUNG DER CMA3-FAERBUNG
......................................................................
49
4.6
AKRIDINORANGE-TEST.............................................................................................
51
4.6.1 PROBLEMATIK DER AKRIDINORANGEFAERBUNG
............................................................
51
4.6.2 FINANZIELLER UND ZEITLICHER AUFWAND DES
AOT..........................................52
4.8.3 AUSWERTUNG DES A O T
.......................................................................................
52
4.9 MODIFIZIERTER FLUORESZENZMIKROSKOPISCHER
SPERMIENCHROMATINSTRUKTUR-ASSAY
(MFSCSA).......................................................................................................................
55
4.9.1 PROBLEMATIK DES
MFSCSA.................................................................................55
4.9.2 FINANZIELLER UND ZEITLICHER AUFWAND DES MFSCSA
......................................
56
4.9.3 AUSWERTUNG DES
MFSCSA.................................................................................56
4.10 TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE-MEDIATED DEOXYURIDINE
TRIPHATE-NICK
END LABELING ASSAY
(TUNEL)........................................................................................
57
4.10.1 PROBLEMATIK DES
TUNEL-TESTS.........................................................................57
4.10.2 FINANZIELLER UND ZEITLICHER AUFWAND DES TUNEL-TESTS
.................................
56
4.10.3 AUSWERTUNG DES
TUNEL-TESTS.........................................................................56
4.11
CASPASE-3-NACHWEIS.......................................................................................
62
4.11.1 PROBLEMATIK DES CASPASE-3-NACHWEISES 62
4.11.2 FINANZIELLER U. ZEITLICHER AUFWAND D. CASPASE-3-NACHWEISES
62
4.11.3 AUSWERTUNG DES CASPASE-3-NACHWEISES
.........................................................
62
4.12 ERGEBNISSE DER COMPUTERASSISTIERTEN
SAMENANALYSE.....................................65
4.13 STATISTISCHE
AUSWERTUNG.....................................................................................67
5
DISKUSSION........................................................................................................
79
5.1 DISKUSSION DER
FRAGESTELLUNG............................................................................79
5.2 DISKUSSION DER METHODEN
.................................................................................
84
5.3 DISKUSSION DER
ERGEBNISSE................................................................................
94
5.3.1
ANILINBLAU.............................................................................................................94
5.3.2
TOLUIDINBLAU........................................................................................................
96
5.3.3 CHROMOMYCIN
A3................................................................................................
99
5.3.4
AKRIDINORANGETEST.............................................................................................
102
5.3.5
MFSCSA............................................................................................................
106
5.3.6
TUNEL..............................................................................................................
108
5.3.7
CASPASE-3-AKTIVITAET..........................................................................................111
5.4 FAZIT FUER DIE VETERINAERMEDIZINISCHE P
RAXIS.......................................................112
6
ZUSAMMENFASSUNG.........................................................................................115
7
SUMMARY..........................................................................................................117
8
LITERATURVERZEICHNIS......................................................................................
119
9
ANHANG.............................................................................................................
149
10
DANKSAGUNG....................................................................................................
159
|
| any_adam_object | 1 |
| author | Laux, Elisa-Marie |
| author_facet | Laux, Elisa-Marie |
| author_role | aut |
| author_sort | Laux, Elisa-Marie |
| author_variant | e m l eml |
| building | Verbundindex |
| bvnumber | BV044897364 |
| ctrlnum | (OCoLC)999269605 (DE-599)DNB1137337915 |
| dewey-full | 630 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 630 - Agriculture and related technologies |
| dewey-raw | 630 |
| dewey-search | 630 |
| dewey-sort | 3630 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
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| indexdate | 2024-07-10T08:04:09Z |
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| institution_GND | (DE-588)1065553803 |
| isbn | 9783835965874 3835965875 |
| language | German |
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| publishDate | 2017 |
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| publisher | VVB Laufersweiler Verlag |
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| spelling | Laux, Elisa-Marie Verfasser aut UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN Elisa-Marie Laux 201707 Gießen, Lahn VVB Laufersweiler Verlag 2017 txt rdacontent n rdamedia nc rdacarrier Edition Scientifique Dissertation Justus Liebig Univerität 2017 chromatin cabt condensation cabt dogs cabt Spermien cabt Doktorarbeit Uni Wissenschaft (DE-588)4113937-9 Hochschulschrift gnd-content VVB LAUFERSWEILER Verlag (DE-588)1065553803 pbl DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030291237&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Laux, Elisa-Marie UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN chromatin cabt condensation cabt dogs cabt Spermien cabt |
| subject_GND | (DE-588)4113937-9 |
| title | UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN |
| title_auth | UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN |
| title_exact_search | UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN |
| title_full | UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN Elisa-Marie Laux |
| title_fullStr | UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN Elisa-Marie Laux |
| title_full_unstemmed | UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN Elisa-Marie Laux |
| title_short | UNTERSUCHUNGEN ZUR CHROMATINKONDENSATION CANINER SPERMATOZOEN |
| title_sort | untersuchungen zur chromatinkondensation caniner spermatozoen |
| topic | chromatin cabt condensation cabt dogs cabt Spermien cabt |
| topic_facet | chromatin condensation dogs Spermien Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030291237&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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