Methods in extracellular matrix biology:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA ; San Diego, CA ; Oxford ; London
Academic Press
2018
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in Cell Biology
Volume 143 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xxi, 446 Seiten Illustrationen, Diagramme 24 cm |
| ISBN: | 9780128122976 0128122978 |
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| 245 | 1 | 0 | |a Methods in extracellular matrix biology |c edited by Robert P. Mecham, Washington University School of Medicine, St Louis, MO, United States |
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Datensatz im Suchindex
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| adam_text | Contents
Contributors..........................................................xiii
Preface................................................................xix
CHAPTER t Ultrastructural analysis of the extracellular matrix..........1
Douglas R. Keene, Sara F. Tufa
1. Introduction..............................................2
2. Methods..................................................5
3. Materials...............................................35
References..................................................36
CHAPTER 2 Live tissue antibody injection: A novel method
for imaging ECM in limb buds and other tissues.......41
Donald A. Fowler, Michael B. Filla, Charles D. Little,
Brenda J. Rongish, Hans C.E. Larsson
1. Introduction......................................... 42
2. Method..................................................43
3. Results.................................................47
4. Summary.................................................53
References..................................................53
CHAPTER 3 Nonlinear optical imaging of extracellular matrix
proteins.................................................57
Chien-Cheng Shih, Dennis M. Oakley, Matthew S. ioens,
Robyn A. Roth, James A.J. Fitzpatrick
1. Introduction......................................... 58
2. Methods............................................. 61
3. Imaging the Microstructure of Mice Aorta Using MHIM.....66
4. Conclusions.............................................74
Acknowledgments.............................................74
References..................................................75
CHAPTER 4 Methods for the visualization and analysis of
extracellular matrix protein structure and
degradation..............................................79
Annemarie K. Leonard, Elizabeth A. Loughran, Yuliya Klymenko,
Yueying Liu, Oleg Kim, Marwa Asem, Kevin McAbee,
Matthew J. Ravosa, M. Sharon Stack
1. Introduction............................................ 80
2. Advanced Imaging for Visualization of Collagen
and Other ECM Proteins in Tissues........................80
VI
Contents
3. Analysis of Cellular Interaction With Collagen-Rich Matrices ..87
4. Zymography..............................................91
Acknowledgments............................................94
References.................................................94
CHAPTER 5 Cell-derived decellularized extracellular matrices....97
Greg M. Harris, Irene Raitman, Jean E. Schwarzbauer
1- Introduction............................................98
2. Decellularization of Cell-Derived ECM..................100
3. Assessment of Decellularized Matrix....................103
4. Exploiting a Decellularized Matrix.....................107
5. Conclusions............................................109
Acknowledgments...........................................110
References................................................110
CHAPTER 6 Analysis of collagen and elastin cross-links.................ns
Mitsuo Yamauchi, Yuki Taga, Shunji Hattori,
Masashi Shiiba, Masahiko Terajima
1. Introduction...........................................116
2. Materials............................................ 119
3. Methods................................................122
4. Notes..................................................126
Acknowledgment............................................129
References................................................130
CHAPTER 7 Measurement of elastin, collagen, and total
protein levels in tissues..............................133
Ivan Stoilov, Barry C. Starcher, Robert P. Mecham,
Thomas J. Broekelmann
1. Introduction...........................................134
2. Acid Hydrolysis and Total Protein Measurement..........134
3. Elastin: Immunoassay for Desmosine.....................136
4. Collagen: Hydroxyproline Measurement...................142
5. Analysis...............................................144
Acknowledgments...........................................144
References................................................144
CHAPTER 8 Measurement of lysyl oxidase activity from
small tissue samples and cell cultures.................147
Philip C. Trackman, Manish V. Bais
1. Introduction...........................................148
2. Equipment..............................................150
3. Solutions..............................................150
Contents
4. Procedures...............................................152
References..................................................156
CHAPTER 9 Microtiter assays for quantitation of assembly
of plasma and cellular fibronectin.......................157
Bianca R. Tomasini-Johansson, Deane F. Mosher
1. Background and Rationale for Assays......................158
2. Examples of Assays.......................................160
3. Method...................................................162
; Acknowledgments.............................................168
d References..................................................168
CHAPTER 10 Basement membrane collagen IV: Isolation of
functional domains.........................................m
Sergei P. Boudko, Neonila Danylevych, Billy G. Hudson,
Vadim K. Pedchenko
1. Introduction.............................................172
2. Isolation of 7S and NCI Domains of Collagen IV
From Solid Tissues.......................................174
3. Isolation of 7S and NCI Domains From ECM Deposited
by Cultured Cells........................................177
4. Isolation of Collagenous Domain by Limited Pepsin
^ „ Digestion.................................................178
5. Recombinant Expression of Collagen IV Fragments..........179
6. Dissociation and Assembly of Collagen IV NCI Hexamers.....182
7. Conclusion............................................. 183
Acknowledgments.............................................184
References..................................................184
■ ՛՛! c
CHAPTER 11 Isolation and analysis of laminins.......................187
Monique Aumailley
1. Introduction.............................................188
2. Isolation of Laminins....................................191
3. Analysis of Laminins in Tissue and Cells.................195
4. Test Cell Adhesion-Promoting Activity of Laminin.........200
Acknowledgments.............................................203
References..................................................203
Further Reading.............................................205
CHAPTER 12 Elastin purification and solubilization..................207
Carmen M. Halabi, Robert P. Mecham
1. Introduction.............................................208
2. Isolation of Cross-Linked, Insoluble Elastin From Tissues.210
VIII
Contents
3. Assessment of Elastin Levels in Tissues...................213
4. Histological Stains for Elastin in Tissue Sections........215
5. Solubilized Forms of Insoluble Elastin....................217
6. Conclusions...............................................219
Acknowledgments...............................................219
References....................................................220
CHAPTER 13 Fibrillins....................................................223
Heena Kumra, Dieter P. Reinhardt
1. Introduction and Rationale................................225
2. Isolation of Microfibrils.................................226
3. Recombinant Production of Fibrillins......................229
4. Knockdown of Fibrillins in Cell Culture...................232
5. Immunoblotting of Fibrillins..............................235
6. Protein Interaction Assay for Fibrillins..................235
7. Analysis of Fibrillins and Their Assembly in Cell Culture.237
8. Analysis of Fibrillins in Tissue by Immunofluorescence....239
9. Analysis of Fibrillins in Tissue by Immunohistochemistry..240
Acknowledgments...............................................243
References....................................................244
CHAPTER 14 Expression and purification of recombinant
fibulins in mammalian cells...............................247
Katsuhiro Hanada, Takako Sasaki
1. Introduction..............................................248
2. Recombinant Expression of Fibulins........................250
3. Purification of Recombinant Fibulins......................256
References.................................................. 258
CHAPTER 15 The biochemistry and immunohistochemistry
of versican...............................................26i
Stephen P. Evanko, Christina K. Chan, Pamela Y. Johnson,
Charles W. Frevert, Thomas N. Wight
1. Introduction..............................................262
2. Isolation, Purification, and Characterization of Versican
From Bovine Aorta....................................... 263
3. Isolation of Versican From Cell Cultures..................268
4. Characterization of the Versican Preparations by Western
Blotting................................................. 269
5. Localization of Versican in Tissue Using
Immunohistochemistry......................................272
6. Quantitation of Versican on Tissue Sections...............273
Acknowledgments...............................................276
References....................................................276
Contents
ix
CHAPTER 16 Isolation, production, and analysis of small
leucine-rich proteoglycans in bone.......................2Si
David J. Kirby, Marian F. Young
1. Introduction............................................282
2. Isolation of Protein From Bone..........................283
3. Isolation of Proteoglycans From Conditioned Media.......289
4. Treatment With Chondroitinase and Assessment of SLRPs...292
5. Summary.................................................294
References................................ ................295
Further Reading.............................................296
CHAPTER 17 Quantification of hyaluronan (HA) using a
simplified fluorophore-assisted carbohydrate
electrophoresis (FACE) procedure.........................297
Ronald J. Midura, Valbona Cali, Mark E. Lauer,
Anthony Calabro, Vincent C. Hascall
1. Introduction............................................298
2. Principle and Theory....................................301
3. Equipment...............................................302
4. Materials (High Quality, Reagent Grade).................305
5. Solutions...............................................306
6. AMAC Labeling of HA and CS Disaccharides................308
7. Gel Preparation.........................................311
8p Image Acquisition........................................313
9. Data Normalization.......................................315
References..................................................315
CHAPTER 18 Isolation and functional analysis of syndecans......317
Pyong Woo Park
1- Introduction........................................... 318
2. Isolation of Syndecans..................................319
3. Functional Analysis.....................................325
Acknowledgments.............................................330
References..................................................330
CHAPTER 19 Production and purification of recombinant
human SPARC..............................................335
Gail Workman, Amy D. Bradshaw
1. Introduction............................................336
2. Method..................................................337
3. Purification of Recombinant SPARC Produced by
Insect Cells.............................................339
References..................................................343
X
Contents
CHAPTER 20 Thrombospondins: Purification of human
platelet thrombospondin-1..............................347
John M. Sipes, Joanne E. Murphy-Ullrich, David D. Roberts
1. Introduction...........................................348
2. Preparation of Thrombospondin-1 From Human Platelets..350
3. Nondenaturing Removal of TGFß..........................361
References............................................... 366
CAHPTER 21 How to detect and purify tenascin-C.......................371
Sean P. Giblin, Devadarssen Murdamoothoo, Claire Deligne,
Anja Schwenzer, Gertraud Orend, Kim S. Midwood
1. Introduction...........................................372
2. Analysis of Tenascin-C mRNA............................373
3. Analysis of Tenascin-C Protein—Western Blotting........380
4. Analysis of Tenascin-C Protein—Tissue Staining.........383
5. Analysis of Tenascin-C Protein—ELISA...................386
6. Purification of His-Tagged Recombinant Tenascin-C......389
7. Knockdown of Tenascin-C Expression.....................394
8. Preparation of Cell-Derived Matrix
by Fibroblasts Expressing or Lacking TNC...............396
9. Summary................................................398
Acknowledgments...........................................398
References................................................398
CHAPTER 22 Investigating cell-type specific functions
of tenascin-C..........................................401
Devadarssen Murdamoothoo, Anja Schwenzer,
Jessica Kant, Tristan Rupp, Anna Marzeda, Kim Midwood,
Gertraud Orend
1. Introduction...........................................402
2. Adhesion Assay.........................................403
3. Analysis of Actin Stress Fibers........................405
4. Analysis of Cell Survival..............................406
5. Analysis of Cell Proliferation.........................408
6. Analysis of Cell Migration.............................410
7. Analysis of Endothelial Cell Branching.................411
8. Analysis of Pseudotube Formation.......................412
9. Analysis of Neovascularization.........................414
10. Analysis of Immune Cell Behavior......................420
Acknowledgments...........................................427
References................................................427
Contents xi
CHAPTER 23 Matrilins..................................................429
Mats Paulsson, Raimund Wagener
1. Introduction...........................................430
2. Methods................................................434
3. Matrilin Antibody Generation...........................441
4. Conclusions............................................442
Acknowledgments...........................................442
References................................................442
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| spelling | Methods in extracellular matrix biology edited by Robert P. Mecham, Washington University School of Medicine, St Louis, MO, United States First edition Cambridge, MA ; San Diego, CA ; Oxford ; London Academic Press 2018 xxi, 446 Seiten Illustrationen, Diagramme 24 cm txt rdacontent n rdamedia nc rdacarrier Methods in Cell Biology Volume 143 Methode (DE-588)4038971-6 gnd rswk-swf Ultrastruktur (DE-588)4061568-6 gnd rswk-swf Extrazelluläre Matrix (DE-588)7530993-2 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Extracellular matrix (DE-588)4143413-4 Aufsatzsammlung gnd-content Extrazelluläre Matrix (DE-588)7530993-2 s Ultrastruktur (DE-588)4061568-6 s Methode (DE-588)4038971-6 s DE-604 Biochemische Analyse (DE-588)4255721-5 s Mecham, Robert P. 1948- (DE-588)1151842168 edt Methods in Cell Biology Volume 143 (DE-604)BV002534878 143 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030119173&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Methods in extracellular matrix biology Methods in Cell Biology Methode (DE-588)4038971-6 gnd Ultrastruktur (DE-588)4061568-6 gnd Extrazelluläre Matrix (DE-588)7530993-2 gnd Biochemische Analyse (DE-588)4255721-5 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4061568-6 (DE-588)7530993-2 (DE-588)4255721-5 (DE-588)4143413-4 |
| title | Methods in extracellular matrix biology |
| title_auth | Methods in extracellular matrix biology |
| title_exact_search | Methods in extracellular matrix biology |
| title_full | Methods in extracellular matrix biology edited by Robert P. Mecham, Washington University School of Medicine, St Louis, MO, United States |
| title_fullStr | Methods in extracellular matrix biology edited by Robert P. Mecham, Washington University School of Medicine, St Louis, MO, United States |
| title_full_unstemmed | Methods in extracellular matrix biology edited by Robert P. Mecham, Washington University School of Medicine, St Louis, MO, United States |
| title_short | Methods in extracellular matrix biology |
| title_sort | methods in extracellular matrix biology |
| topic | Methode (DE-588)4038971-6 gnd Ultrastruktur (DE-588)4061568-6 gnd Extrazelluläre Matrix (DE-588)7530993-2 gnd Biochemische Analyse (DE-588)4255721-5 gnd |
| topic_facet | Methode Ultrastruktur Extrazelluläre Matrix Biochemische Analyse Aufsatzsammlung |
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