Verfügbarkeit: Practical manual on plant cytogenetics

Practical manual on plant cytogenetics:

Gespeichert in:

Bibliographische Detailangaben
1. Verfasser:	Singh, Ram J. (VerfasserIn)
Format:	Buch
Sprache:	English
Veröffentlicht:	Boca Raton CRC Press [2018]
Schlagworte:	Plant cytogenetics > Laboratory manuals Plant genetics > Laboratory manuals Pflanzenzelle Methode Cytogenetik
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	Includes bibliographical references
Beschreibung:	xxv, 320 Seiten Illustrationen
ISBN:	9781498742979

Internformat

MARC


LEADER	00000nam a2200000 c 4500
001	BV044721911
003	DE-604
005	20180315
007	t
008	180119s2018 xxua\|\|\| \|\|\|\| 00\|\|\| eng d
010			\|a 017036705
020			\|a 9781498742979 \|9 978-1-4987-4297-9
035			\|a (OCoLC)1011039708
035			\|a (DE-599)BVBBV044721911
040			\|a DE-604 \|b ger \|e rda
041	0		\|a eng
044			\|a xxu \|c US
049			\|a DE-11 \|a DE-355
050		0	\|a QK981.35
082	0		\|a 572.8/2 \|2 23
084			\|a WC 4440 \|0 (DE-625)148101: \|2 rvk
084			\|a WG 2000 \|0 (DE-625)148512: \|2 rvk
084			\|a WG 4000 \|0 (DE-625)148559: \|2 rvk
100	1		\|a Singh, Ram J. \|e Verfasser \|0 (DE-588)172688205 \|4 aut
245	1	0	\|a Practical manual on plant cytogenetics \|c Ram J. Singh
246	1	3	\|a Plant cytogenetics
264		1	\|a Boca Raton \|b CRC Press \|c [2018]
300			\|a xxv, 320 Seiten \|b Illustrationen
336			\|b txt \|2 rdacontent
337			\|b n \|2 rdamedia
338			\|b nc \|2 rdacarrier
500			\|a Includes bibliographical references
650		4	\|a Plant cytogenetics \|v Laboratory manuals
650		4	\|a Plant genetics \|v Laboratory manuals
650	0	7	\|a Pflanzenzelle \|0 (DE-588)4115551-8 \|2 gnd \|9 rswk-swf
650	0	7	\|a Methode \|0 (DE-588)4038971-6 \|2 gnd \|9 rswk-swf
650	0	7	\|a Cytogenetik \|0 (DE-588)4070176-1 \|2 gnd \|9 rswk-swf
689	0	0	\|a Pflanzenzelle \|0 (DE-588)4115551-8 \|D s
689	0	1	\|a Cytogenetik \|0 (DE-588)4070176-1 \|D s
689	0	2	\|a Methode \|0 (DE-588)4038971-6 \|D s
689	0		\|5 DE-604
856	4	2	\|m Digitalisierung UB Regensburg - ADAM Catalogue Enrichment \|q application/pdf \|u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030118167&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA \|3 Inhaltsverzeichnis
999			\|a oai:aleph.bib-bvb.de:BVB01-030118167

Datensatz im Suchindex

_version_	1804178210866331649
adam_text	Contents Foreword..................................................................xix Preface...................................................................xxi Acknowledgments.........................................................xxiii Author....................................................................xxv Chapter 1 Introduction....................................................1 Chapter 2 Conventional Methods for Handling Plant Chromosomes...........11 2.1 Introduction..........................................11 2.2 Mitotic Chromosomes...................................11 2.2.1 Collection of Roots.............................11 2.2.2 Pretreatment of Roots...........................13 2.2.2.1 Ice-Cold Water.........................13 2.2.2.2 8-Hydroxyquinoline (C9H7NO)........... 14 2.2.23 Colchicine (C22H2506N).................14 2.2.2.4 cx-Bromonaphthalene (C10H7Br)..........15 2.2.2.5 p-Dichlorobenzene (C6H4Ci2)............15 2.2.2.6 Nitrous Oxide (N20)................ 16 2.2.3 Fixation........................................17 2.2.3.1 Carnoy’s Solution I....................17 2.23.2 Carnoy’s Solution II...................18 2.23.3 Propionic Acid Alcohol.................18 2.2.4 Staining of Mitotic Chromosomes.................18 2.2.4.1 Preparation of Stain................. 18 2.2.4.2 Staining of Roots......................21 2.2.5 Preparation of Chromosome Spread................23 2.2.5.1 Supplies............................. 23 2.2.5.2 Chromosome Spreading...................23 2.2.53 Giemsa Staining........................32 2.3 Meiotic Chromosomes....................................38 23.1 Collection of Flower Buds.......................38 2.3.2 Fixation...................................... 40 23.2.1 Carnoy’s Solution 1....................40 23.2.2 Carnoy’s Solution 2....................40 2.3.23 Propionic Acid Alcohol.................40 2.3.3 Staining of Meiotic Chromosomes.................41 23.4 Preparation of Chromosome Slide...................41 2.3.5 Observation of Cells............................41 2.4 Supplies...............................................43 References...................................................44 vii Contents VIII Chapter 3 Smear Technique for Plant Chromosomes...........................47 3.1 Introduction............................................47 3.2 Chromosome Preparation from the Root...................47 3.2.1 Enzyme Treatment.................................47 3.2.2 Preparation of Chromosome Spread.................48 3.3 Chromosome Preparation from Cell Suspension and Callus.............................................48 3.3.1 Chromosome Count in Suspension Culture...........49 3.3.2 Chromosome Count in Callus.......................49 3.4 Chromosome Count from Leaf Explants.....................50 3.4.1 Potato...........................................50 3.4.2 Beetroot..............................-........51 3.4.3 Rose.............................................53 3.4.4 Banana...........................................55 3.4.5 Coffee...........................................56 3.4.6 Ag-NOR Banding.................................. 57 3.4.7 Hsc-FA Banding...................................57 3.4.8 Image Analysis...................................58 3.5 Chromosome Preparation from Flowers.....................59 3.5.1 Meiotic Chromosomes..............................59 References....................................................60 Chapter 4 Fluorescence In Situ Hybridization...............................63 4.1 Introduction........................................... 63 4.2 Equipment...............................................63 4.3 DNA Isolation...........................................63 4.3.1 Buffer/Tissue....................................64 4.3.2 Procedure........................................64 4.4 Nick Translation.......................................66 4.4.1 Nick Translation System..........................66 4.4.2 Nick Translation Kit.............................66 4.4.3 Labeling Reaction with DIG-dUTP or Biotin-dUTP..66 4.4.4 Labeling Reaction with Fluorescein dUTP, AM C A-dUTP, or Tetramethyl-rhodamine-dUTP......................67 4.4.5 Purification of Labeled Probe....................68 4.4.6 Nick Translation Labeling of Double-Stranded DNA with DIG-, Biotin-, or Fluorochrome- Labeled dUTP....................................68 4.5 Preparation of Buffers.................................69 4.5.1 Preparation of CA—SC Buffer......................69 4.5.2 Preparation of SSC Buffer........................69 4.5.3 Preparation of 4x SSC + 0.2% Tween 20............69 4.5.4 Preparation of 2x SSC............................69 Contents IX 4.6 Preparation of Enzyme Solution............................69 4.7 Preparation of Slides for Soybean Chromosomes.............69 4.7.1 Processing Slides through FISH....................70 4.7.1.1 Pre-hybridization Method................71 4.7.1.2 Hybridization...........................71 4.7.1.3 Observation and Photography.............72 4.8 Preparation of FISH Slides for Wheat Chromosomes..........73 4.8.1 Chromosome Preparation............................73 4.8.2 Procedure of FISH/GISH............................74 4.8.2.1 Preparation of Stock Solution for FISH and GISH.................................74 4.8.2.2 Purification of Labeled Probe DNA.......75 4.8.2.3 Random Primed DNA Labeling with Digoxigenin-dUTP.........................77 4.8.2.4. Random Primed DIG Labeling of DNA Fragment.............................78 4.8.3 Immunofluorescence of CENH3 of Wheat Centromere..................................78 4.8.3.1 Plant Materials and Chromosome Preparation...................78 4.8.3.2 Immunodetection of CENH3 and FISH..........79 4.8.3.3 Sequential Detection of CENH3, CRWs, pScll9, and pAsl...................80 4.8.3.4 Genome-Specific Markers and PCR.........80 4.9 Genomic In Situ Hybridization........................... 80 4.9.1 DNA Isolation.....................................81 4.9.1.1 CTAB Method.............................81 4.9.1.2 Selective Precipitation of Polysaccharides.83 4.9.1.3 DNA Quantification......................83 4.9.1.4 Nick Translation........................84 4.9.2 Collection of Roots...............................85 4.9.3 Genomic In Situ Hybridization.....................85 4.9.4 Preparation of GISH Hybridization Mixture.........86 4.9.5 Posthybridization.................................86 4.10 Multicolor Genomic In Situ Hybridization (McGISH).........87 4.11 Multicolor FISH for Wheat Genome..........................89 4.11.1 Genomic Library Construction......................89 4.11.1.1 Slide Preparation.......................90 4.11.1.2 Probe Labeling Using VentR (exo-) Polymerase...............................92 4.11.1.3 FISH and Reprobing Procedures...........93 4.11.2 Primed In Situ (Prins) DNA Labeling...............94 4.11.3 FISH on Extended DNA Fiber: Fiber-FISH............95 4.11.3.1 Protoeol-I: Fiber-FISH on Extended Nuclear DNA Fibers.......................96 X Contents 4.11.3.2 Protocol 2: Fiber-FISH Using BAC and Circular Molecules as Targets.........100 4.11.3.3 Protocol III: Staining Fibers (Yo-Yo Staining)......................100 4.11.3.4 Source of Chemicals..................101 References................................................. 101 Chapter 5 Flow Analysis and Sorting of Plant Chromosomes...............103 5.1 Introduction..........................................103 5.2 Basic Protocol 1......................................103 5.2.1 Accumulation of Root-Tip Cells in Mitotic Metaphase.............................103 5.2.2 Materials.....................................107 5.2.3 Germination of Seeds .........................107 5.2.4 Accumulation of Root-Tip Cells in Metaphase..108 5.3 Alternate Protocol 1..................................108 5.3.1 Accumulation of Root-Tip Cells in Mitotic Metaphase in Large-Seeded Legumes.............108 5.3.2 Additional Materials..........................108 5.4 Alternate Protocol 2..................................109 5.4.1 Accumulation of Root-Tip Cells at the Mitotic Metaphase by Nitrous Oxide Treatment..........109 5.4.2 Additional Materials..........................109 5.5 Alternate Protocol 3..................................109 5.5.1 Accumulation of Root-Tip Cells at the Mitotic Metaphase in Plant Species Where Seeds are Unavailable...............................109 5.5.2 Additional Materials..........................109 5.5.3 Grow the Roots................................109 5.5.4 Prepare for Treatments........................110 5.6 Support Protocol 1................................. 110 5.6.1 Analysis of the Degree of Metaphase Synchrony.... 110 5.6.2 Materials.................................... 110 5.6.3 Fix and Stain Cells.......................... Ill 5.6.4 Prepare and Analyze Squashes................. Ill 5.7 Basic Protocol 2................................... 112 5.7.1 Preparation of Suspensions of Intact Plant Chromosomes.................................. 112 5.7.2 Materials.................................... 112 5.7.3 Prepare Chromosome Suspension.................112 5.7.4 Examine Quality of Chromosomes................113 5.8 Basic Protocol 3..................................... 113 5.8.1 Univariate Flow Karyotyping of Plant Chromosomes.................................. 113 Contents xi 5.8.2 Materials.................................... 113 5.8.3 Perform Flow Cytometry....................... 114 5.8.4 Sort Chromosomes............................. 114 5.9 Alternate Protocol 4................................ 115 5.9.1 Bivariate Flow Karyotyping of Plant Chromosomes after FISHIS .................... 115 5.9.2 Materials.................................... 115 5.9.3 Label DNA Repeats............................ 115 5.9.4 Perform Flow Cytometry....................... 116 5.9.5 Sort Chromosomes............................. 116 5.10 Support Protocol 2.................................. 116 5.10.1 Preparation of Flow Sorter for Chromosome Sorting........................... 116 5.10.2 Additional Materials......................... 117 5.10.3 Set Up Flow Cytometer........................ 117 5.10.4 Adjust Sorting Device........................ 117 5.11 Basic Protocol 4.................................... 117 5.11.1 Chromosome Sorting for DNA Amplification..... 117 5.11.2 Materials.................................... 118 5.11.3 Sort Chromosomes............................. 118 5.11.4 Purify and Amplify DNA....................... 118 5.12 Alternate Protocol 5................................ 119 5.12.1 Single Chromosome Sorting and Amplification . 119 5.12.2 Materials.................................... 119 5.12.3 Sort Chromosomes............................. 119 5.12.4 Amplify DNA.................................. 119 5.13 Alternate Protocol 6.................................120 5.13.1 Chromosome Sorting for Preparation of High Molecular Weight DNA (HMW DNA) ...............120 5.13.2 Materials.....................................120 5.13.3 Prepare HMW DNA from Flow-Sorted Chromosomes.......................120 5.14 Alternate Protocol 7................................ 121 5.14.1 Chromosome Sorting for Proteomic Analyses.... 121 5.14.2 Materials.....................................121 5.14.3 Methods...................................... 121 5.15 Support Protocol 3.................................. 121 5.15.1 Estimation of Purity in Sorted Fractions Using FISH................................... 121 5.15.2 Materials.....................................122 5.15.3 Sort Chromosomes..............................122 5.15.4 Perform FISH Reaction.........................122 5.15.5 Reagents and Solutions........................123 5.15.5.1 Amiprophos-methyl (APM) Treatment Solutions..................123 XII Contents 5.15.5.2 Blocking Reagent......................123 5.15.5.3 DAPI (4 6-Diamidino-2-phenylindole) Stock Solution, 0.1 mg/mL..............123 5.15.5.4 ET Buffer.............................124 5.15.5.5 Formaldehyde Fixative.................124 5.15.5.6 Fructose Syrup........................124 5.15.5.7 Hoagland’s Nutrient Solution, O.lx...124 5.15.5.8 Hoagland’s Nutrient Solution, lx.....125 5.15.5.9 Hoagland’s Solution A.................125 5.15.5.10 Hoagland’s Solution B.................125 5.15.5.11 Hoagland’s Solution C.................125 5.15.5.12 Hoagland’s Stock Solution, lOx........125 5.15.5.13 Hybridization Mix (FISH)..............125 5.15.5.14 Hydroxyurea Treatment Solutions.......126 5.15.5.15 Isolation Buffer, 1.5x (1.5x IB)......126 5.15.5.16 Isolation Buffer, lOx (lOx IB)........126 5.15.5.17 LB01 Buffer...........................126 5.15.5.18 LB01-P Buffer, lx.....................127 5.15.5.19 LB01-P Buffer, lOx....................127 5.15.5.20 Lysis Buffer (Single Chromosome Amplification).......................127 5.15.5.21 Lysis Buffer B (HMW DNA)..............127 5.15.5.22 Lysis Buffer C (HMW DNA)..............128 5.15.5.23 NaOH Solution, 10 M...................128 5.15.5.24 Neutralization Buffer (Single Chromosome Amplification)............128 5.15.5.25 Oryzalin Solutions....................128 5.15.5.26 P5 Buffer.............................128 5.15.5.27 Phenylmethanesulfonylfluoride (PMSF) Stock Solution, 100 mM........128 5.15.5.28 Proteinase K Buffer, 40x..............129 5.15.5.29 Proteinase K Stock Solution, 10 mg/mL............................ 129 5.15.5.30 Schiff’s Reagent.....................129 5.15.5.31 SSC Solutions........................129 5.15.5.32 Tris Buffer..........................129 5.15.5.33 Tris Cl, 1M..........................129 5.16 Commentary.............................................130 5.16.1 Background Information.........................130 5.16.2 Materials Used for Preparation of Plant Chromosome Suspensions..........................132 5.16.3 Cell Cycle Synchronization and Accumulation of Metaphase Chromosomes...........................132 5.16.4 Preparation of Chromosome Suspensions..........133 5.16.5 Flow Karyotyping...............................134 5.16.6 Chromosome Sorting.............................135 Contents xîiî 5.16.7 Utility of Sorted Chromosome Fractions..........135 5.16.8 Critical Parameters and Troubleshooting.........138 5.16.8.1 Cell Cycle Synchronization and Metaphase..........................138 5.16.8.2 Preparation of Chromosome Suspension ....139 5.16.8.3 Flow Karyotyping.......................140 5.16.8.4 Chromosome Sorting....................141 5.16.8.5 Estimation of Purity of Sorted Fractions using FISH...................141 5.16.8.6 Multiple Displacement Amplification of Chromosomal DNA.....................142 5.16.8.7 Single Chromosome Sorting and Amplification..........................142 5.16.8.8 Preparation of HMW DNA.................143 5.16.8.9 Proteomic Analyses.....................143 5.17 Anticipated Results....................................143 5.17.1 Preparation of Chromosome Suspensions...........143 5.17.2 Flow Karyotyping and Chromosome Sorting........ 143 5.17.3 Determining the Purity of Sorted Chromosome Fractions Using FISH............................144 5.17.4 DNA Amplification...............................145 5.17.5 Preparation of HMW DNA..........................145 5.17.6 Time Considerations.............................145 5.17.7 Preparation of Chromosome Suspensions...........145 5.17.8 FISHIS..........................................146 5.17.9 Flow Analysis and Sorting.......................146 5.17.10 Estimating the Purity of Sorted Chromosomes...146 5.17.11 Single Chromosome DNA Amplification............146 5.17.12 Preparation of HMW DNA.........................147 5.17.13 Proteomic Analyses.............................147 References..................................................147 Chapter 6 Pollen Staining.................................................155 6.1 Introduction...........................................155 6.2 Protocols for Pollen Fertility.........................155 6.2.1 Acetocarmine Stain..............................155 6.2.1.1 Fertile Pollen Grains..................155 6.2.1.2 Sterile Pollen Grains..................158 6.2.2 Iodine-Potassium Iodide (I2-KI Stain)...........158 6.2.3 Alexander Stain.................................158 6.2.3.1 Ingredients............................160 6.2.3.2 Staining...............................161 6.2.3.3 Stain Solution........................ 161 6.2.4 Differential Staining of Pollen ................164 6.2.4.1 Staining of Pollen with Thin Wall......164 XIV Contents 6.2.4.2 Staining of Pollen with Thick and Spiny Walls............................164 6.2.43 Staining of Pollen inside a Nondehiscent Anther....................165 63 Pollen-Stigma Incompatibility..........................165 63.1 Pollen Germination Test.........................165 63.1.1 Method.................................166 63.1.2 Pollen Culture.........................166 63.1.3 Humidity Chamber.......................166 63.1.4 Microscopic Examination................167 63.2 Staining Pistils with Aniline Blue..............169 63.3 Cleared-Pistil Method...........................170 63.4 Modified Cleared-Pistil Method................. 173 6.4 Chromosome Count in Pollen ............................173 6.4.1 Stain...........................................173 References...................................................176 Chapter 7 Cell Division.................................................177 7.1 Introduction......................................... 177 7.2 Mitosis................................................177 7.2.1 Process of Mitosis..............................177 7.2.1.1 Interphase.............................177 7.2.1.2 Prophase...............................179 7.2.13 Metaphase............................ 179 7.2.1.4 Anaphase...............................179 7.2.1.5 Telophase..............................179 7.2.1.6 Cytokinesis............................180 73 Meiosis................................................181 7.3.1 Process of Meiosis..............................181 73.1.1 Cycle 1................................181 73.1.2 Cycle 2................................185 7.4 Gametogenesis..........................................185 7.5 Fertilization........................................ 187 References...................................................187 Chapter 8 Mode of Reproduction in Plants..................................189 8.1 Sexual Reproduction....................................189 8.2 Asexual Reproduction...................................189 8.2.1 Types of Apomixis...............................191 8.2.1.1 Gametophytic Apomixis..................191 8.2.1.2 Apospory Apomixis......................192 8.2.1.3 Adventitious Embryony..................192 8.2.1.4 Polyembryony ..........................193 8.2.2 Endosperm Development in Apomixis...............193 8.2.2.1 Autonomous Endosperm Development.... 193 Contents xv 8.2.3 Pseudogamous Endosperm Development............193 8.2.4 Identification of Apomixis....................194 8.3 Classification of Plants Based on Natural Crossing...194 8.4 Apomixis in Crop Improvement.........................197 8.5 Vegetative Reproduction..............................197 8.5.1 Vegetative Reproduction by Specialized Vegetative Organs.............................197 8.5.1.1 Bulb..................................197 8.5.1.2 Corm..................................197 8.5.1.3 Runners (Stolons).....................197 8.5.1.4 Rhizomes..............................200 8.5.1.5 Tubers................................201 8.5.2 Vegetative Reproduction by Adventitious Roots and Shoots.................................. 203 8.5.3 Vegetative Reproduction by Grafting...........203 8.5.4 Vegetative Reproduction by Tissue Culture.....205 8.5.5 Advantages of Vegetative Reproduction.........205 8.6 Methodologies for Producing Amphidiploidy............206 8.6.1 Colchicine....................................206 8.6.2 Nitrous Oxide.................................207 8.7 Barriers to Crossability in Plants...................208 References.................................................208 Chapter 9 Karyotype Analysis........................................ 209 9.1 Introduction.........................................209 9.2 Nomenclature of Chromosomes..........................209 9.3 Karyotype Analysis by Mitotic Metaphase Chromosomes..211 9.3.1 Chromosome 1 (7H)...........................211 9.3.2 Chromosome 2 (2H)...........................212 9.3.3 Chromosome 3 (3H)...........................212 9.3.4 Chromosome 4 (4H)...........................213 9.3.5 Chromosome 5 (1H)...........................213 9.3.6 Chromosome 6 (6H)...........................213 9.3.7 Chromosome 7 (5H)...........................213 9.4 Karyotype Analysis by Pachytene Chromosomes .........213 9.5 Karyotype Analysis by Flow Cytometry.................214 9.6 Karyotype Analysis by Image Analysis.................216 References.................................................217 Chapter 10 Classical Methods for Associating Genes with the Chromosomes............................................219 10.1 Introduction.........................................219 10.2 Monohybrid Inheritance................................220 10.2.1 Complete Dominance............................220 10.2.2 Incomplete Dominance..........................220 xvi Contents 10.3 Dihybrid Inheritance...................................222 10.4 Gene Interaction and Expression..........................224 10.4.1 Lethal Genes.....................................224 10.4.2 Complementary Genes..............................225 10.4.3 Epistasis........................................225 10.4.3.1 Dominant Epistasis (12:3:1).............225 10.4.3.2 Dominant Suppression Epistasis (13:3)...226 10.4.4 Color Reversion (9:3:4)..........................227 10.4.5 Complete Dominance at Both Gene Pairs (15:1)...227 10.5 Test of Independence.....................................229 10.5.1 Two Independent Nongenetic Events— Two-Coin Tosses..................................229 10.5.2 Four Independent Nongenetic Events— Four-Coin Tosses.................................230 10.6 Genetic Mapping of Chromosomes..........................232 10.6.1 Classical Method.................................232 10.6.2 Chromosome Mapping...............................234 10.6.2.1 Crossing Over and Crossover Frequency...............................234 10.6.2.2 Three-Point Testcross...................236 10.6.2.3 Interference and Coincidence............237 10.7 Rise and Decline of Plant Cytogenetics...................238 References....................................................239 Chapter 11 Structural Chromosome Changes for Locating the Genes............241 11.1 Deficiencies.............................................241 11.1.1 Origin of Deficiencies......................... 241 11.1.2 Identification of Deficiencies...................241 11.1.3 Genetic Studies..................................241 11.2 Duplications.............................................242 11.2.1 Classification of Duplications...................242 11.2.2 Origin of Duplications...........................242 11.2.3 Genetic Studies..................................243 11.3 Chromosomal Interchanges.................................243 11.3.1 Origin of Interchanges...........................243 11.3.2 Identification...................................243 11.3.2.1 Cytological Method......................243 11.3.2.2 Pollen Fertility........................245 11.3.3 Genetic Studies..................................245 11.3.4 Principles of Producing Interchange Testers......246 11.4 Trisomics................................................246 11.4.1 Primary Trisomics................................247 11.4.1.1 Identification of Primary Trisomics....247 11.4.2 Secondary Trisomics..............................250 11.4.2.1 Identification of Secondary Trisomics..251 Contents xvii 11.4.2.2 Genetic Segregation in Secondary Trisomies....................251 11.4.3 Tertiary Trisomies..............................251 11.4.3.1 Identification of Tertiary Trisomies...252 11.4.3.2 Genetic Segregation in Tertiary Trisomies.....................253 11.4.4 Telotrisomics...................................253 11.4.4.1 Identification of Telotrisomics........254 11.4.4.2 Genetic Segregation in Telotrisomics...254 11.4.5 Acrotrisomics...................................257 11.4.5.1 Identification of Acrotrisomics........257 11.4.5.2 Genetic Segregation in Acrotrisomics...258 References....................................................258 Chapter 12 Wide Hybridization..............................................261 12.1 Introduction............................................261 12.2 Gene Pool Concept.......................................261 12.3 Utilization of the Tertiary Gene Pool................. 263 12.4 The Assemblage of Large Germplasm.......................263 12.5 Wide Hybridization in Soybean...........................264 12.5.1 Selection of Parents in Wide Hybridization......264 12.5.2 Emasculation....................................265 12.5.3 Embryo Rescue...................................267 12.5.4 Production of and Amphidiploid Plants...........271 12.5.5 Production of BCL and BC2 Plants................272 12.5.6 Production of BC3F1 Plants......................274 12.5.7 Production of BC3F2 Plants and Beyond...........274 12.5.8 Isolation of Monosomie Alien Addition Lines.....274 12.5.9 Broadening the Cytoplasm of the Soybean.........274 12.6 Introgression of Useful Genes through Distant Hybridization...................................279 References....................................................279 Glossary...................................................................281 Index......................................................................313
any_adam_object	1
author	Singh, Ram J.
author_GND	(DE-588)172688205
author_facet	Singh, Ram J.
author_role	aut
author_sort	Singh, Ram J.
author_variant	r j s rj rjs
building	Verbundindex
bvnumber	BV044721911
callnumber-first	Q - Science
callnumber-label	QK981
callnumber-raw	QK981.35
callnumber-search	QK981.35
callnumber-sort	QK 3981.35
callnumber-subject	QK - Botany
classification_rvk	WC 4440 WG 2000 WG 4000
ctrlnum	(OCoLC)1011039708 (DE-599)BVBBV044721911
dewey-full	572.8/2
dewey-hundreds	500 - Natural sciences and mathematics
dewey-ones	572 - Biochemistry
dewey-raw	572.8/2
dewey-search	572.8/2
dewey-sort	3572.8 12
dewey-tens	570 - Biology
discipline	Biologie
format	Book
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01815nam a2200481 c 4500</leader><controlfield tag="001">BV044721911</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180315 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">180119s2018 xxua\|\|\| \|\|\|\| 00\|\|\| eng d</controlfield><datafield tag="010" ind1=" " ind2=" "><subfield code="a">017036705</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781498742979</subfield><subfield code="9">978-1-4987-4297-9</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1011039708</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044721911</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">xxu</subfield><subfield code="c">US</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-11</subfield><subfield code="a">DE-355</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QK981.35</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.8/2</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4440</subfield><subfield code="0">(DE-625)148101:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 2000</subfield><subfield code="0">(DE-625)148512:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 4000</subfield><subfield code="0">(DE-625)148559:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Singh, Ram J.</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)172688205</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Practical manual on plant cytogenetics</subfield><subfield code="c">Ram J. Singh</subfield></datafield><datafield tag="246" ind1="1" ind2="3"><subfield code="a">Plant cytogenetics</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Boca Raton</subfield><subfield code="b">CRC Press</subfield><subfield code="c">[2018]</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">xxv, 320 Seiten</subfield><subfield code="b">Illustrationen</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Includes bibliographical references</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Plant cytogenetics</subfield><subfield code="v">Laboratory manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Plant genetics</subfield><subfield code="v">Laboratory manuals</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Pflanzenzelle</subfield><subfield code="0">(DE-588)4115551-8</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Cytogenetik</subfield><subfield code="0">(DE-588)4070176-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Pflanzenzelle</subfield><subfield code="0">(DE-588)4115551-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Cytogenetik</subfield><subfield code="0">(DE-588)4070176-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg - ADAM Catalogue Enrichment</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030118167&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030118167</subfield></datafield></record></collection>
id	DE-604.BV044721911
illustrated	Illustrated
indexdate	2024-07-10T08:00:21Z
institution	BVB
isbn	9781498742979
language	English
lccn	017036705
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-030118167
oclc_num	1011039708
open_access_boolean
owner	DE-11 DE-355 DE-BY-UBR
owner_facet	DE-11 DE-355 DE-BY-UBR
physical	xxv, 320 Seiten Illustrationen
publishDate	2018
publishDateSearch	2018
publishDateSort	2018
publisher	CRC Press
record_format	marc
spelling	Singh, Ram J. Verfasser (DE-588)172688205 aut Practical manual on plant cytogenetics Ram J. Singh Plant cytogenetics Boca Raton CRC Press [2018] xxv, 320 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references Plant cytogenetics Laboratory manuals Plant genetics Laboratory manuals Pflanzenzelle (DE-588)4115551-8 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Cytogenetik (DE-588)4070176-1 gnd rswk-swf Pflanzenzelle (DE-588)4115551-8 s Cytogenetik (DE-588)4070176-1 s Methode (DE-588)4038971-6 s DE-604 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030118167&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Singh, Ram J. Practical manual on plant cytogenetics Plant cytogenetics Laboratory manuals Plant genetics Laboratory manuals Pflanzenzelle (DE-588)4115551-8 gnd Methode (DE-588)4038971-6 gnd Cytogenetik (DE-588)4070176-1 gnd
subject_GND	(DE-588)4115551-8 (DE-588)4038971-6 (DE-588)4070176-1
title	Practical manual on plant cytogenetics
title_alt	Plant cytogenetics
title_auth	Practical manual on plant cytogenetics
title_exact_search	Practical manual on plant cytogenetics
title_full	Practical manual on plant cytogenetics Ram J. Singh
title_fullStr	Practical manual on plant cytogenetics Ram J. Singh
title_full_unstemmed	Practical manual on plant cytogenetics Ram J. Singh
title_short	Practical manual on plant cytogenetics
title_sort	practical manual on plant cytogenetics
topic	Plant cytogenetics Laboratory manuals Plant genetics Laboratory manuals Pflanzenzelle (DE-588)4115551-8 gnd Methode (DE-588)4038971-6 gnd Cytogenetik (DE-588)4070176-1 gnd
topic_facet	Plant cytogenetics Laboratory manuals Plant genetics Laboratory manuals Pflanzenzelle Methode Cytogenetik
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030118167&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT singhramj practicalmanualonplantcytogenetics AT singhramj plantcytogenetics

Verfügbarkeit

Es ist kein Print-Exemplar vorhanden.

Fernleihe Bestellen Achtung: Nicht im THWS-Bestand! Inhaltsverzeichnis

MARC

Datensatz im Suchindex

Es ist kein Print-Exemplar vorhanden.

Ähnliche Einträge