Enzyme-linked Immunosorbent Assay (ELISA): From A to Z
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Singapore
Springer Singapore
2018
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| Schriftenreihe: | SpringerBriefs in Applied Sciences and Technology
|
| Schlagworte: | |
| Online-Zugang: | DE-634 DE-1046 DE-Aug4 DE-573 DE-M347 DE-92 DE-898 DE-859 DE-860 DE-861 DE-863 DE-862 DE-523 DE-91 DE-706 URL des Erstveröffentlichers Inhaltsverzeichnis |
| Beschreibung: | 1 Online-Ressource (XI, 115 p. 38 illus., 34 illus. in color) |
| ISBN: | 9789811067662 |
| ISSN: | 2191-530X |
| DOI: | 10.1007/978-981-10-6766-2 |
Internformat
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| 245 | 1 | 0 | |a Enzyme-linked Immunosorbent Assay (ELISA) |b From A to Z |c by Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa |
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| 650 | 4 | |a Biomedical engineering | |
| 650 | 4 | |a Engineering | |
| 650 | 4 | |a Biomedical Engineering | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Biological and Medical Physics, Biophysics | |
| 650 | 4 | |a Protein-Ligand Interactions | |
| 650 | 4 | |a Biotechnology | |
| 650 | 4 | |a Computational Biology/Bioinformatics | |
| 700 | 1 | |a Vázquez-Villegas, Patricia |e Sonstige |0 (DE-588)1152415077 |4 oth | |
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Datensatz im Suchindex
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Contents
1 Fundamentals and History of ELISA: The Evolution of the
Immunoassays Until Invention of ELISA 1
1 1 Evolution of the Immunoassays Until Invention of ELISA 1
111 Side Chain Theory 1
112 Antigen-Antibody Binding Theory 2
113 Discovery of Antibody Structure 3
114 Invention of Radioimmunoassay (RIA) 3
115 Invention of Enzyme Linked Immunosorbent Assay
(ELISA) 4
1 2 Principles of the Immune System 5
121 Antibody Production in Human Body 5
122 Different Types of Antibodies 5
123 Antigen-Antibody Coupling 10
1 3 Biomolecular Interactions Between Antibody and Antigen 11
131 Hydrogen Bonding 11
132 Hydrophobic Interaction 12
133 Ionic Attraction 12
134 Van der Waals Forces 14
References 15
2 General Overviews on Applications of ELISA 19
2 1 Applications of ELISA 19
211 Food Industry 19
212 Vaccine Development 20
213 Immunology 20
214 Diagnosis 22
215 Toxicology 24
216 Drug Monitoring and Pharmaceutical Industry 25
217 Transplantation 25
References 26
IX
X
Contents
3 Step by Step with ELISA: Mechanism of Operation, Crucial
Elements, Different Protocols, and Insights on Immobilization
and Detection of Various Biomolecular Entities 31
3 1 Mechanism of Operation 31
3 2 Different Elements of the Assay 33
321 Solid Phase 33
322 Adsorbents 33
323 Washing Agents 34
324 Blocking Agents 34
325 Enzymes and Substrates 34
326 Stopping Process 40
327 Reading Techniques 42
328 Reading Apparatus 43
329 Controls 44
3 3 Different Protocols 45
331 Direct ELISA 45
332 Indirect ELISA 45
333 Sandwich ELISA 45
334 Double Sandwich ELISA 47
335 Competitive ELISA 47
3 4 Initial Interaction of the Biomolecules with the Surface 47
3 5 Immobilization Techniques for Protein Attachment 49
351 Physical Immobilization 49
352 Immobilization via Entrapment 50
353 Covalent Immobilization 50
354 Oriented Immobilization 52
References 53
4 Evaluation of the Detection Results Obtained from ELISA 57
4 1 Conducting a Reliable Assay 57
411 Sources of Errors 58
412 Troubleshooting 58
4 2 Key Parameters in ELISA Evaluation 62
421 Sensitivity 63
422 Specificity 63
423 Accuracy 63
424 Limit of Detection (LOD) 64
4 3 Measurable Units in ELISA 64
References 65
5 Advantages, Disadvantages and Modifications of Conventional
ELISA 67
5 1 Significance of Conventional ELISA 68
5 2 Shortages of Conventional ELISA 68
Contents xi
5 3 Materials of Choice for Fabrication of ELISA Well Plates 69
5 4 Different Types of ELISA Well Plates 70
5 5 Modified ELISA Platforms 71
551 ELISA on Coated Platforms 72
552 ELISpot 74
553 Plasmonic ELISA 76
554 Sphere-/Bead-Based ELISA 80
555 Paper-Based ELISA 84
556 Fiber-Based ELISA 90
557 ELISA in Micro-Devices 98
558 Other Strategies 105 |
| any_adam_object | 1 |
| author | Hosseini, Samira 1979- |
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| discipline | Elektrotechnik Medizin Maschinenbau |
| doi_str_mv | 10.1007/978-981-10-6766-2 |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| indexdate | 2024-10-16T04:00:56Z |
| institution | BVB |
| isbn | 9789811067662 |
| issn | 2191-530X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030099185 |
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| physical | 1 Online-Ressource (XI, 115 p. 38 illus., 34 illus. in color) |
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| publishDate | 2018 |
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| publishDateSort | 2018 |
| publisher | Springer Singapore |
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| series2 | SpringerBriefs in Applied Sciences and Technology |
| spellingShingle | Hosseini, Samira 1979- Enzyme-linked Immunosorbent Assay (ELISA) From A to Z Engineering Immunology Biotechnology Bioinformatics Proteins Biomedical engineering Biomedical Engineering Biological and Medical Physics, Biophysics Protein-Ligand Interactions Computational Biology/Bioinformatics |
| title | Enzyme-linked Immunosorbent Assay (ELISA) From A to Z |
| title_auth | Enzyme-linked Immunosorbent Assay (ELISA) From A to Z |
| title_exact_search | Enzyme-linked Immunosorbent Assay (ELISA) From A to Z |
| title_full | Enzyme-linked Immunosorbent Assay (ELISA) From A to Z by Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa |
| title_fullStr | Enzyme-linked Immunosorbent Assay (ELISA) From A to Z by Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa |
| title_full_unstemmed | Enzyme-linked Immunosorbent Assay (ELISA) From A to Z by Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa |
| title_short | Enzyme-linked Immunosorbent Assay (ELISA) |
| title_sort | enzyme linked immunosorbent assay elisa from a to z |
| title_sub | From A to Z |
| topic | Engineering Immunology Biotechnology Bioinformatics Proteins Biomedical engineering Biomedical Engineering Biological and Medical Physics, Biophysics Protein-Ligand Interactions Computational Biology/Bioinformatics |
| topic_facet | Engineering Immunology Biotechnology Bioinformatics Proteins Biomedical engineering Biomedical Engineering Biological and Medical Physics, Biophysics Protein-Ligand Interactions Computational Biology/Bioinformatics |
| url | https://doi.org/10.1007/978-981-10-6766-2 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=030099185&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hosseinisamira enzymelinkedimmunosorbentassayelisafromatoz AT vazquezvillegaspatricia enzymelinkedimmunosorbentassayelisafromatoz AT ritopalomaresmarco enzymelinkedimmunosorbentassayelisafromatoz AT martinezchapaserigioo enzymelinkedimmunosorbentassayelisafromatoz |