High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins:
The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Singapore
World Scientific Pub. Co.
c2010
|
| Schriftenreihe: | Methods in chromatography
v. 2 |
| Schlagworte: | |
| Online-Zugang: | FHN01 Volltext |
| Zusammenfassung: | The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field |
| Beschreibung: | xii, 232 p. ill. (some col.) |
| ISBN: | 9789814277198 |
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| 520 | |a The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field | ||
| 650 | 4 | |a Porphyrins | |
| 650 | 4 | |a Chlorophyll | |
| 650 | 4 | |a Tetrapyrroles | |
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| author | Lim, Chang Kee |
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| dewey-full | 547.593 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 547 - Organic chemistry |
| dewey-raw | 547.593 |
| dewey-search | 547.593 |
| dewey-sort | 3547.593 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie |
| format | Electronic eBook |
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| id | DE-604.BV044637751 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:57:51Z |
| institution | BVB |
| isbn | 9789814277198 |
| language | English |
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| physical | xii, 232 p. ill. (some col.) |
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| series2 | Methods in chromatography |
| spelling | Lim, Chang Kee Verfasser aut High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins Chang Kee Lim Singapore World Scientific Pub. Co. c2010 xii, 232 p. ill. (some col.) txt rdacontent c rdamedia cr rdacarrier Methods in chromatography v. 2 The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field Porphyrins Chlorophyll Tetrapyrroles High performance liquid chromatography Mass spectrometry Erscheint auch als Druck-Ausgabe 9789810230685 Erscheint auch als Druck-Ausgabe 9810230680 http://www.worldscientific.com/worldscibooks/10.1142/3411#t=toc Verlag URL des Erstveroeffentlichers Volltext |
| spellingShingle | Lim, Chang Kee High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins Porphyrins Chlorophyll Tetrapyrroles High performance liquid chromatography Mass spectrometry |
| title | High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins |
| title_auth | High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins |
| title_exact_search | High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins |
| title_full | High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins Chang Kee Lim |
| title_fullStr | High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins Chang Kee Lim |
| title_full_unstemmed | High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins Chang Kee Lim |
| title_short | High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins |
| title_sort | high performance liquid chromatography and mass spectrometry of porphyrins chlorophylls and bilins |
| topic | Porphyrins Chlorophyll Tetrapyrroles High performance liquid chromatography Mass spectrometry |
| topic_facet | Porphyrins Chlorophyll Tetrapyrroles High performance liquid chromatography Mass spectrometry |
| url | http://www.worldscientific.com/worldscibooks/10.1142/3411#t=toc |
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