Immunocharacteristics of testicular germ cell cancers: revelation of a new key network?
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
VVB Laufersweiler Verlag
2017
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Edition Scientifique
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | viii, 115 Seiten Illustrationen, Diagramme |
| ISBN: | 9783835965294 3835965298 |
Internformat
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| 245 | 1 | 0 | |a Immunocharacteristics of testicular germ cell cancers |b revelation of a new key network? |c submitted by Britta Klein |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Gießen |b VVB Laufersweiler Verlag |c 2017 | |
| 300 | |a viii, 115 Seiten |b Illustrationen, Diagramme | ||
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| 502 | |b Dissertation |c Universität Gießen |d 2016 | ||
| 650 | 7 | |a neoplasms |2 cabt | |
| 650 | 7 | |a gem cells |2 cabt | |
| 650 | 7 | |a testicular diseases |2 cabt | |
| 650 | 7 | |a testicular cancer |2 cabt | |
| 650 | 7 | |a male genitalia |2 cabt | |
| 650 | 7 | |a spermatozoa |2 cabt | |
| 650 | 7 | |a immunology |2 cabt | |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
CHAPTER 1 1
1 INTRODUCTION 1
1 1 Characteristics of human testicular germ cell tumours (TGCTs) 1
111 Epidemiology 1
112 Aetiology 2
113 Diagnosis and treatment 4
1 2 The testis and its immune privilege 5
121 Immunological characteristics of the human testis - under
physiological conditions 5
122 Immunological characteristics of the human testis - under
pathological conditions 6
1 3 Studying the tumour microenvironment (TME) 7
131 From simple presence to biological and functional meaning - significance
and role of tumour infiltrating,lymphocytes (TIL) 7
132 Cytokines and chemokines as a way to stratify roles and immunological
activities of cells within the tumour microenvironment 8
133 Interaction between cancer cells and immune cells - in vitro analysis of
intercellular communication 10
CHAPTER 2: Analysis of immune cell involvement under testicular inflammatory conditions
and TGCTs 12
2 INTRODUCTION 13
2 1 MATERIALS AND METHODS 13
211 Patients 13
212 Histological evaluation of testicular biopsies 13
213 Immunohistochemistry 14
2 2 RESULTS 18
221 Immunohistochemistry revealed different patterns of immune cell involve-
ment and quantities associated with different levels of disruption of the
physiological testicular environment 18
222 Advanced stages of testicular cancer were associated with an increased
involvement of dendritic cells and the unique presence of B cells 27
2 3 DISCUSSION 34
CHAPTER 3: Analysis of cytokine transcript profiles under testicular inflammatory condi-
tions and TGCTs 40
3 INTRODUCTION 41
3 1 MATERIALS AND METHODS 41
311 Patients 41
312 Histological evaluation of testicular biopsies 41
313 Reverse transcriptase PCR (RT-PCR) 42
314 Quantitative real-time RT-PCR 42
3 2 RESULTS 44
321 RT-qPCR identified pathology-specific cytokine transcript profiles 44
322 The presence of even few CIS cells might already change/shape the
overall cytokine microenvironment present 47
3 3 DISCUSSION 48
CHAPTER 4: Establishment of an in vitro model for the study of human TGCTs 55
4 INTRODUCTION 56
4 1 MATERIALS AND METHODS 56
411 Cell cocultures 56
412 Reverse transcriptase PCR (RT-PCR) 58
413 Quantitative real-time RT-PCR 58
414 ELISA analysis 59
415 Check for amount of remaining PBMC in TCam-2 cell fractions
post-coculture 60
4 2 RESULTS 61
421 Sample processing procedure applied in this study allows good separation of
TCam-2 cell and PBMC cell fractions post-coculture for subsequent inde-
pendent analysis 61
422 TCam-2 cell proliferation appeared to be unaffected by
coculture with PBMC 63
423 Cocultured PBMC showed increased proliferation compared to monocul-
tured controls 63
424 PBMC did not respond to colocalisation with TCam-2 cells by an
increase in cytokine transcript levels 64
425 TCam-2 cells responded to direct colocalisation with PBMC by an
increase in cytokine transcript levels 65
426 RT-qPCR revealed a correlation between TCam2-cell cytokine response and
the strength and type of communication/interaction with PBMC
during coculture 67
427 TCam-2 cells identified as having high IL6 baseline transcript levels 69
428 Results of IL6 ELISA analyses on culture media were in line with
observations on the RNA-based TCam-2 cell characteristics of IL6 71
4 3 DISCUSSION 72
5 SCHEMATIC SUMMARY of the key outcomes describing different events during TGCT
progression 77
6 CONCLUSION 78
7 APPENDICES 79
7 1 APPENDIX CHAPTER 2 82
7 2 APPENDIX CHAPTER 3 89
7 3 APPENDIX CHAPTER 4 92
8 ABSTRACT 95
9 ZUSAMMENFASSUNG 98
10 REFERENCES 101
11 ACKNOWLEDGEMENT 112
12 PUBLICATIONS 113
13 DECLARATION 115
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| indexdate | 2024-07-10T07:54:22Z |
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| isbn | 9783835965294 3835965298 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-029889980 |
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| physical | viii, 115 Seiten Illustrationen, Diagramme |
| publishDate | 2017 |
| publishDateSearch | 2017 |
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| spelling | Klein, Britta Verfasser (DE-588)1128651491 aut Immunocharacteristics of testicular germ cell cancers revelation of a new key network? submitted by Britta Klein 1. Auflage Gießen VVB Laufersweiler Verlag 2017 viii, 115 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Edition Scientifique Dissertation Universität Gießen 2016 neoplasms cabt gem cells cabt testicular diseases cabt testicular cancer cabt male genitalia cabt spermatozoa cabt immunology cabt (DE-588)4113937-9 Hochschulschrift gnd-content HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029889980&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Klein, Britta Immunocharacteristics of testicular germ cell cancers revelation of a new key network? neoplasms cabt gem cells cabt testicular diseases cabt testicular cancer cabt male genitalia cabt spermatozoa cabt immunology cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Immunocharacteristics of testicular germ cell cancers revelation of a new key network? |
| title_auth | Immunocharacteristics of testicular germ cell cancers revelation of a new key network? |
| title_exact_search | Immunocharacteristics of testicular germ cell cancers revelation of a new key network? |
| title_full | Immunocharacteristics of testicular germ cell cancers revelation of a new key network? submitted by Britta Klein |
| title_fullStr | Immunocharacteristics of testicular germ cell cancers revelation of a new key network? submitted by Britta Klein |
| title_full_unstemmed | Immunocharacteristics of testicular germ cell cancers revelation of a new key network? submitted by Britta Klein |
| title_short | Immunocharacteristics of testicular germ cell cancers |
| title_sort | immunocharacteristics of testicular germ cell cancers revelation of a new key network |
| title_sub | revelation of a new key network? |
| topic | neoplasms cabt gem cells cabt testicular diseases cabt testicular cancer cabt male genitalia cabt spermatozoa cabt immunology cabt |
| topic_facet | neoplasms gem cells testicular diseases testicular cancer male genitalia spermatozoa immunology Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029889980&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kleinbritta immunocharacteristicsoftesticulargermcellcancersrevelationofanewkeynetwork |