Difference gel electrophoresis: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
[2018]
|
| Schriftenreihe: | Methods in molecular biology
1664 |
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | x, 315 Seiten Illustrationen, Diagramme |
| ISBN: | 9781493972678 1493972677 |
Internformat
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Datensatz im Suchindex
| _version_ | 1806414018916646912 |
|---|---|
| adam_text |
Titel: Difference gel electrophoresis
Autor: Ohlendieck, Kay
Jahr: 2018
Contents
Preface. v
Contributors. ix
Part I Introduction
1 Two-Dimensional Gel Electrophoresis and 2D-DIGE. 3
Paula Meleady
Part II Difference Gel Electrophoresis
2 Comparative DIGE Proteomics. 17
Kay Ohlendieck
3 2D-DIGE and Fluorescence Image Analysis. 25
Elisa Robotti and Emilio Marengo
4 DIGE Analysis Software and Protein Identification Approaches. 41
Abduladim Hmmier and Paul Dowling
Part III DIGE Applications
5 Native DIGE: Efficient Tool to Elucidate Protein Interactomes. 53
Diksha Dani and Norbert A. Dencher
6 Comparative Two-Dimensional Fluorescence Gel Electrophoresis. 69
Doreen Ackermann and Simone König
7 DIGE-Based Phosphoproteomic Analysis. 79
Taras Stasyk and Lukas Alfons Huber
8 DIGE Saturation Labeling for Scarce Amounts of Protein
from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue. 87
Paul Dowling
9 Comparative 3-Sample DIGE Analysis of Skeletal Muscles. 93
Kay Ohlendieck
10 DIGE Analysis of ProteoMinerâ„¢ Fractionated Serum/Plasma Samples. 109
Sandra Murphy and Paul Dowling
Part IV Comparative DIGE Tissue Proteomics
11 DIGE Analysis of Human Tissues. 117
Cecilia Gelfi and Daniele Capitanio
12 DIGE Analysis of Animal Tissues. 137
Alessio Di Luca, Ruth Hamill, Anne Maria Mullen, and Giuliano Elia
13 Rapid 2D DIGE Proteomic Analysis of Mouse Liver. 153
Shotaro Kamata and Isao Ishii
vii
viii
Contents
14 Proteomic Analysis of Lung Tissue by DIGE. 167
Jarlath E. Nally and Simone Schuller
15 Comparative Testis Tissue Proteomics Using 2-Dye Versus
3-Dye DIGE Analysis. 185
Ashling Holland
16 DIGE Analysis of Fish Tissues. 203
Joanna Nynca, Mariola A. Dietrich, and Andrzej Ciereszko
Part V Auxiliary DIGE Methods
17 Protein Digestion for DIGE Analysis. 223
Sandra Murphy and Kay Ohlendieck
18 Subcellular Fractionation for DIGE-Based Proteomics. 233
Sandra Murphy
19 DIGE Analysis of Immunodepleted Plasma. 245
Paul Dowling and Kay Ohlendieck
Part VI DIGE Verification Analysis
20 Elucidating Cellular Metabolism and Protein Difference Data
from DIGE Proteomics Experiments Using Enzyme Assays. 261
Andrew Dowd
21 Enzyme Assay Methods to Validate DIGE Proteomics Data. 279
Andrew Dowd
22 Immunoblot Analysis of DIGE-Based Proteomics. 287
Martin Landsberger and Heinrich Brinkmeier
23 Immunofluorescence Microscopy for DIGE-Based Proteomics. 301
Rustam R. Mundegar, Margit Zweyer, and Dieter Swandulla
Index
311 |
| any_adam_object | 1 |
| author2 | Ohlendieck, Kay |
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| dewey-full | 570 |
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| dewey-ones | 570 - Biology |
| dewey-raw | 570 |
| dewey-search | 570 |
| dewey-sort | 3570 |
| dewey-tens | 570 - Biology |
| discipline | Chemie / Pharmazie Biologie |
| format | Book |
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| spelling | Difference gel electrophoresis methods and protocols edited by Kay Ohlendieck, Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland New York, NY Springer New York [2018] Humana Press [2018] x, 315 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1664 Springer protocols Gelelektrophorese (DE-588)4123267-7 gnd rswk-swf fluorescence image analysis proteomic profiling PTM analysis Immunoblot Gelelektrophorese (DE-588)4123267-7 s DE-604 Ohlendieck, Kay (DE-588)1143137280 edt Erscheint auch als Online-Ausgabe, eBook 978-1-4939-7268-5 Methods in molecular biology 1664 (DE-604)BV035362695 1664 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=7f6c3361a9d84dc7a0878ee1c636e787&prov=M&dok_var=1&dok_ext=htm Inhaltstext HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029888225&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Difference gel electrophoresis methods and protocols |
| title_auth | Difference gel electrophoresis methods and protocols |
| title_exact_search | Difference gel electrophoresis methods and protocols |
| title_full | Difference gel electrophoresis methods and protocols edited by Kay Ohlendieck, Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland |
| title_fullStr | Difference gel electrophoresis methods and protocols edited by Kay Ohlendieck, Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland |
| title_full_unstemmed | Difference gel electrophoresis methods and protocols edited by Kay Ohlendieck, Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland |
| title_short | Difference gel electrophoresis |
| title_sort | difference gel electrophoresis methods and protocols |
| title_sub | methods and protocols |
| topic | Gelelektrophorese (DE-588)4123267-7 gnd |
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