Membrane protein structure and function characterization: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2017]
|
| Schriftenreihe: | Methods in molecular biology
1635 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | xii, 419 Seiten Illustrationen, Diagramme 25.4 cm x 17.8 cm |
| ISBN: | 9781493971497 |
Internformat
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| 245 | 1 | 0 | |a Membrane protein structure and function characterization |b methods and protocols |c edited by Jean-Jacques Lacapère |
| 264 | 1 | |a New York, NY |b Humana Press |c [2017] | |
| 264 | 4 | |c © 2017 | |
| 300 | |a xii, 419 Seiten |b Illustrationen, Diagramme |c 25.4 cm x 17.8 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 1635 | |
| 490 | 0 | |a Springer protocols | |
| 653 | |a PSBC ; BISAC Subject Heading | ||
| 653 | |a membrane protein | ||
| 653 | |a folded protein | ||
| 653 | |a membrane mimicking environments | ||
| 653 | |a functional status | ||
| 653 | |a antibodies | ||
| 653 | |a PSBC ; BIC subject category | ||
| 700 | 1 | |a Lacapère, Jean-Jacques |4 edt | |
| 710 | 2 | |a Springer Science + Business Media LLC |0 (DE-588)1065492340 |4 pbl | |
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| 830 | 0 | |a Methods in molecular biology |v 1635 |w (DE-604)BV035362695 |9 1635 | |
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Datensatz im Suchindex
| _version_ | 1804177808473194496 |
|---|---|
| adam_text | Titel: Membrane protein structure and function characterization
Autor: Lacapère, Jean-Jacques
Jahr: 2017
Contents
Preface..................................................................... p
Contributors................................................................. ix
1 Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2......... 1
Lucile Senicourt, Soria Iatmanen-Harbi, Claude Hattab,
Mariano Anibal Ostuni, Marie-France Giraud, and Jean-Jacques Lacapere
2 Functional Assembly of Soluble and Membrane Recombinant Proteins
of Mammalian NADPH Oxidase Complex................................. 27
Hajer Souabni, Aymen Ezzine, Tania Bizouarn, and Laura Baciou
3 Direct Extraction and Purification of Recombinant Membrane
Proteins from Pichia pastoris Protoplasts................................... 45
Lucie Hartmann, Estelle Metzger, Noémie Ottelard,
and Renaud Wagner
4 Cell-Free Expression for the Study of Hydrophobic Proteins:
The Example of Yeast ATP-Synthase Subunits.............................. 57
Isabelle Larrieu, James Tolchard, Corinne Sanchez, Edmond Tazo Kone,
Alexandre Barras, Claire Stines-Chaumeil, Benoît Odaert,
and Marie-France Giraud
5 Wheat Germ Cell-Free Overexpression for the Production
of Membrane Proteins................................................... 91
Marie-Laure Fogeron, Aurélie Badillo, François Penin,
and Anja Böckmann
6 Methyl-Specific Isotope Labeling Strategies for NMR Studies
of Membrane Proteins................................................... 109
Vilius Kurauskas, Paul Schanda, and Remy Sounier
7 Labeling of Membrane Complexes for Electron Microscopy.................. 125
Francesca Gubellini and Rémi Fronzes
8 Expression, Biochemistry, and Stabilization with Camel
Antibodies of Membrane Proteins: Case Study
of the Mouse 5-HT3 Receptor........................................... 139
Ghérici Hassaïne, Cédric Deluz, Luigino Grasso, Romain Wyss,
Ruud Hovius, Henning Stahlberg, Takashi Tomizaki, Aline Desmyter,
Christophe Moreau, Lucie Peclinovska, Sonja Minniberger,
Lamia Mebarki, Xiao-Dan Li, Horst Vogel, and Hugues Nury
9 Characterization of New Detergents and Detergent Mimetics
by Scattering Techniques for Membrane Protein Crystallization.............. 169
Françoise Bonneté and Patrick J. Loll
10 Secondary Structure Determination by Means of ATR-FTIR Spectroscopy____ 195
Batoul Srour, Stefan Bruechert, Susana L.A. Andrade,
and Petra Hellwig
vii
viii
Contents
11 Native Mass Spectrometry for the Characterization of Structure
and Interactions of Membrane Proteins.................................... 205
Jeroen F. van Dyck, Albert Konijnenberg, and Frank Sobott
12 Mass Spectrometry of Mitochondrial Membrane Protein Complexes.......... 233
Luc Negroni, Michel Zivy, and Claire Lemaire
13 Functional Studies on Membrane Proteins by Means
of H/D Exchange in Infrared: Structural Changes in Na+ NQR
from V. cholerae in the Presence of Lipids.................................. 247
Tashvin Neehaul, Sebastien Kriegel, Bianca Barquera,
and Petra Hellwig
14 Reconstitution of Membrane Proteins in Liposomes........................ 259
Alice Verchère, Isabelle Broutin, and Martin Picard
15 Ion Channels as Reporters of Membrane Receptor Function:
Automated Analysis in Xenopus Oocytes................................... 283
Michel Vivaudou, Zlatomir Todorov, Gina Catalina Reyes-Mejia,
and Christophe Moreau
16 The CRACAM Robot: Two-Dimensional Crystallization
of Membrane Protein................................................... 303
Philippe Rosier, Frédéric Gélébart, Nicolas Dumesnil,
Gauthier Esnot, Manuela Dezi, Marc Morand,
and Catherine Vénien-Bryan
17 Reconstitution of Membrane Proteins into Nanodiscs
for Single-Particle Electron Microscopy................................... 317
Laetitia Daury, Jean-Christophe Taveau, Dimitri Salvador,
Marie Glavier, and Olivier Lambert
18 Solid-State NMR of Membrane Protein Reconstituted
in Proteoliposomes, the Case of TSPO.................................... 329
Lucile Senicourt, Luminita Duma, Vassilios Papadopoulos,
and Jean-Jacques Lacapere
19 Sample Preparation for Membrane Protein Structural
Studies by Solid-State NMR.............................................. 345
Denis Lacabanne, Britta Kunert, Carole Gardiennet,
Beat H. Meier, and Anja Böckmann
20 Simulation of Ligand Binding to Membrane Proteins....................... 359
Samuel Murail
21 Molecular Modeling of Transporters: From Low
Resolution Cryo-Electron Microscopy Map to Conformational
Exploration. The Example of TSPO....................................... 383
Aurore Vaitinadapoule and Catherine Etchebest
Index...................................................................... 417
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| physical | xii, 419 Seiten Illustrationen, Diagramme 25.4 cm x 17.8 cm |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Membrane protein structure and function characterization methods and protocols edited by Jean-Jacques Lacapère New York, NY Humana Press [2017] © 2017 xii, 419 Seiten Illustrationen, Diagramme 25.4 cm x 17.8 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1635 Springer protocols PSBC ; BISAC Subject Heading membrane protein folded protein membrane mimicking environments functional status antibodies PSBC ; BIC subject category Lacapère, Jean-Jacques edt Springer Science + Business Media LLC (DE-588)1065492340 pbl Erscheint auch als Online-Ausgabe 978-1-4939-7151-0 Methods in molecular biology 1635 (DE-604)BV035362695 1635 http://digitool.hbz-nrw.de:1801/webclient/DeliveryManager?pid=7344449&custom_att_2=simple_viewer Membrane protein structure and function characterization Inhaltsverzeichnis HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029875198&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Membrane protein structure and function characterization methods and protocols Methods in molecular biology |
| title | Membrane protein structure and function characterization methods and protocols |
| title_auth | Membrane protein structure and function characterization methods and protocols |
| title_exact_search | Membrane protein structure and function characterization methods and protocols |
| title_full | Membrane protein structure and function characterization methods and protocols edited by Jean-Jacques Lacapère |
| title_fullStr | Membrane protein structure and function characterization methods and protocols edited by Jean-Jacques Lacapère |
| title_full_unstemmed | Membrane protein structure and function characterization methods and protocols edited by Jean-Jacques Lacapère |
| title_short | Membrane protein structure and function characterization |
| title_sort | membrane protein structure and function characterization methods and protocols |
| title_sub | methods and protocols |
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