Bacterial protein secretion systems: methods and protocols
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2017]
|
| Schriftenreihe: | Methods of biochemical analysis
1615 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xv, 528 Seiten Illustrationen |
| ISBN: | 9781493970315 |
Internformat
MARC
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| 245 | 1 | 0 | |a Bacterial protein secretion systems |b methods and protocols |c editors Laure Journet and Eric Cascales, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France |
| 264 | 1 | |a New York, NY |b Humana Press |c [2017] | |
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| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Methods of biochemical analysis |v 1615 | |
| 700 | 1 | |a Journet, Laure |4 edt | |
| 700 | 1 | |a Cascales, Eric |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |t Bacterial Protein Secretion Systems |d New York, NY : Humana Press, 2017 |h Online-Ressource (XVI, 528 p. 96 illus., 70 illus. in color, online resource) |z 978-1-4939-7033-9 |
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Datensatz im Suchindex
| _version_ | 1804177805816102912 |
|---|---|
| adam_text | Titel: Bacterial protein secretion systems
Autor: Journet, Laure
Jahr: 2017
Contents
Preface.............................................................. v
Contributors.......................................................... xi
1 Identification of Protein Secretion Systems in Bacterial
Genomes Using MacSyFinder......................................... 1
Sophie S. Abby and Eduardo P.C. Rocba
2 Protein Sorting Prediction........................................... 23
Henrik Nielsen
3 Cell Fractionation.................................................. 59
Melissa Petiti, Laetitia Houot, and Denis Duche
4 Defining Lipoprotein Localisation by Fluorescence Microscopy................ 65
Maria Guillermina Casabona, Mylene Robert-Genthon,
Didier Grunwald, and Ina Attree
5 Identification of Lipoproteins Using Globomycin and
Radioactive Palmitate............................................... 75
Nienke Buddelmeijer
6 Defining Membrane Protein Localization by Isopycnic Density Gradients........ 81
Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow
7 Cell Surface Exposure............................................... 87
Anna Konovalova
8 Probing Inner Membrane Protein Topology by Proteolysis................... 97
Maxence S. Vincent and Eric Cascales
9 Mapping of Membrane Protein Topology by Substituted
Cysteine Accessibility Method (SCAMâ„¢)................................ 105
Mikhail Bogdanov
10 Defining Membrane Protein Topology Using pho-lac Reporter Fusions.......... 129
Gouzel Karimova and Daniel Ladant
11 In Vivo and In Vitro Protein-Peptidoglycan Interactions.................... 143
Gang Li and S. Peter Howard
12 Measure of Peptidoglycan Hydrolase Activity............................. 151
Toann G. Santin and Eric Cascales
13 Protein-Protein Interaction: Bacterial Two-Hybrid......................... 159
Gouzel Karimova, Emilie Gauliard, Marilyne Davi,
Scot P. Ouellette, and Daniel Ladant
14 Protein-Protein Interactions: Yeast Two-Hybrid System..................... 177
Jer-Sheng Lin and Erh-Min Lai
15 Protein-Protein Interactions: Cytology Two-Hybrid........................ 189
Krishnamohan Atmakuri
16 Fusion Reporter Approaches to Monitoring Transmembrane Helix
Interactions in Bacterial Membranes.................................... 199
Laureen Logger, Abdelrahim Zoued, and Eric Cascales
vii
viii Contents
17 Protein-Protein Interactions: Co-Immunoprecipitation..................... 211
Jer-Sheng Lin and Erh-Min Lai
18 Protein-Protein Interaction: Tandem Affinity Purification in Bacteria........... 221
Julie P.M. Viala and Emmanuelle Bouveret
19 Site-Directed and Time-Resolved Photocrosslinking in Cells
Metabolically Labeled with Radioisotopes................................ 233
Rajfaele leva
20 Protein-Protein Interactions: Pull-Down Assays........................... 247
Arthur Louche, Suzana P. Salcedo, and Sarah Bigot
21 Protein-Protein Interactions: Surface Plasmon Resonance.................... 257
Badreddine Douzi
22 Assessing Energy-Dependent Protein Conformational
Changes in the TonB System......................................... 277
Ray A. Larsen
23 Defining Assembly Pathways by Fluorescence Microscopy.................... 289
Abdelrahim Zoued and Andreas Diepold
24 Large Complexes: Cloning Strategy, Production, and Purification.............. 299
Eric Durand and Roland Lloubes
25 Shearing and Enrichment of Extracellular Type IV Pili...................... 311
Alba Katiria Gonzalez Rivera and Katrina T. Forest
26 Blue Native PAGE Analysis of Bacterial Secretion Complexes................. 321
Susann Zilkenat, Tobias Dietsche, Julia V. Monjaras Feria,
Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner
27 In Situ Imaging of Bacterial Secretion Systems by Electron Cryotomography..... 353
Gregor L. Weiss, Jodo M. Medeiros, and Martin Pilhofer
28 Structural Analysis of Protein Complexes by Cryo Electron Microscopy......... 377
Tiago R.D. Costa, Athanasios Ignatiou, and Elena V. Orlova
29 Bacterial Filamentous Appendages Investigated by Solid-State
NMR Spectroscopy................................................. 415
Birgit Habenstein and Antoine Loquet
30 Energy Requirements for Protein Secretion via the
Flagellar Type III Secretion System................................... 449
Marc Erhardt
31 Identification of Effectors: Precipitation of Supernatant Material............... 459
Nicolas Elaugnatti and Laure Journet
32 Screening for Secretion of the Type VI Secretion System Protein Hep
by Enzyme-Linked Immunosorbent Assay and Colony Blot.................. 465
Brent S. Weber, Pek Man Ly, and Mario F. Feldman
33 Effector Translocation: Cya Reporter Assay............................... 473
Suma Chakravarthy, Bethany Huot, and Brian H. Kvitko
34 Monitoring Effector Translocation using the TEM-1 Beta-Lactamase
Reporter System................................................... 489
Julie AUombert, Anne Vianney, and Xavier Charpentier
Contents ix
35 Effector Translocation Assay: Differential Solubilization..................... 501
Irina S. Franco, Sara V. Pais, Nuno Charro, and Luis Jaime Mota
36 Quantitative Determination of Anti-bacterial Activity During
Bacterial Co-culture................................................ 517
Juliana Alcoforado Diniz, Birte Hollmann, and Sarah J. Coulthurst
Index............................................................... 525
|
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| isbn | 9781493970315 |
| language | English |
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| spelling | Bacterial protein secretion systems methods and protocols editors Laure Journet and Eric Cascales, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France New York, NY Humana Press [2017] xv, 528 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods of biochemical analysis 1615 Journet, Laure edt Cascales, Eric edt Erscheint auch als Online-Ausgabe Bacterial Protein Secretion Systems New York, NY : Humana Press, 2017 Online-Ressource (XVI, 528 p. 96 illus., 70 illus. in color, online resource) 978-1-4939-7033-9 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029874035&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bacterial protein secretion systems methods and protocols |
| title | Bacterial protein secretion systems methods and protocols |
| title_auth | Bacterial protein secretion systems methods and protocols |
| title_exact_search | Bacterial protein secretion systems methods and protocols |
| title_full | Bacterial protein secretion systems methods and protocols editors Laure Journet and Eric Cascales, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France |
| title_fullStr | Bacterial protein secretion systems methods and protocols editors Laure Journet and Eric Cascales, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France |
| title_full_unstemmed | Bacterial protein secretion systems methods and protocols editors Laure Journet and Eric Cascales, Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France |
| title_short | Bacterial protein secretion systems |
| title_sort | bacterial protein secretion systems methods and protocols |
| title_sub | methods and protocols |
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