Zymography: methods and protocols
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York NY
Humana Press
[2017]
|
| Schriftenreihe: | Methods in molecular biology
1626 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xx, 310 Seiten Illustrationen, Diagramme |
| ISBN: | 9781493971091 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804177740557975552 |
|---|---|
| adam_text | Titel: Zymography
Autor: Wilkesman, Jeff
Jahr: 2017
Contents
Dedication..............................................................................................................p
In Memoriam..................................................................................................................pH
Preface............................................................................................................................ix
Acknowledgments............................................................................................................xi
Contributors....................................................................................................................xvii
Part I Introduction
1 Zymography Principles.......................................... 3
Jeff Wilkesman and Liliana Kurz
Part II Endopeptidase Zymography
2 Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive
RAMA Casein Zymography...................................... 13
Hidetaro Tasumitsu
3 Cysteine Protease Zymography: Brief Review......................... 25
Jeff Wilkesman
4 Aspartic Protease Zymography Case Study: Detection of Fungal Acid
Proteases by Zymography........................................ 33
Gavin Kernaghan and Michael Mayerhofer
5 Detection of Aspartic Proteinase Activities Using Gel Zymography......... 43
Handunge Kumudu Irani Perera
6 MMP Activity Detection in Zymograms............................. 53
Péter Bencsik, Monika Bartekova, Anikô Görbe, Krisztina Kiss,
Jdnos Pâlôczi, Jana Radosinska, Gergo Szucs, and Péter Perdinandy
7 Characterization of Novel Collagenolytic Proteases..................... 71
Goran Mucic, Brankica Ralkovic, and Natalija Polovic
8 Zymography as a Research Tool in the Study of Matrix Metalloproteinase
Inhibitors.................................................... 79
Zßngli Ren, Juanjuan Chen, and RaoufA. Khalil
9 Detection and Characterization of Bacterial Proteinases Using Zymography . . 103
Madathiparambil G. Madanan and Ambili Mechoor
10 A Sensitive, Rapid, and Specific Technique for the Detection
of Collagenase Using Zymography................................. 115
Shivcharan Prasad and Ipsita Roy
Part III Reverse Zymography and In Situ Zymography
11 Reverse Zymography: Overview and Pitfalls.......................... 125
Kanika Sharma and Debasish Bhattacharyya
xiii
xiv Contents
12 Cell In Situ Zymography: Imaging Enzyme-Substrate Interactions......... 133
Aastha Chhabra and Vibha Rani
Part IV 2D Zymography
13 Examination of Gelatinase Isoforms in Rodent Models
of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography. . 147
Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui,
and Zezong Gu
14 Two-Dimensional Zymography of Proteases from Steatotic Duck Liver...... 157
Jeff Wilkesman, Maria Fernanda Padrôn, Liliana Kurz,
and Hervé Rêmignon
Part V Special Zymography Cases
15 Simultaneous Detection of Activity and Relative Molecular Mass
of Adenylate Kinases After SDS-PAGE and Blotting.................... 169
Silvia Ravera and Isabella Panfoli
16 Silver-Stained Fibrin Zymography: Separation of Proteases
and Activity Detection Using a Single Substrate-Containing Gel........... 179
Chang-Su Park, Dae-Ook Kang, and Nack-Shick Choi
17 Zymography Methods to Simultaneously Analyze Superoxide Dismutase
and Catalase Activities: Novel Application for Yeast Species Identification .... 189
Esther Gamero-Sandemetrio, Rocio GÃ mez-Pastor, and Emilia Matallana
18 Detection of Guaiacol Peroxidase on Electrophoretic Gels................ 199
Diana Castro, Lellys M. Contreras, Liliana Kurz, and Jeff Wilkesman
19 In Situ Demonstration and Characteristic Analysis of the Protease
Using Substrate Immersing Zymography............................. 205
HaiLun He, Hao Li, and Dan Liu
20 Use of Zymography in Trypanosomiasis Studies....................... 213
Jéssyka Fernanda Santiago Monte, Cldudia Jassica Gonçalves Moreno,
Joana Patricia Molato Figueiredo Lopes Monteiro,
Hugo Alexandre de Oliveira Rocha, Aline Rimoldi Ribeiro,
and Marcelo Sousa Silva
21 Zymography in Multiwells for Quality Assessment of Proteinases........... 221
Ambili Mechoor and Madathiparambil G. Madanan
22 Visualization of Enzyme Activities in Earthworm Biopores
by In Situ Soil Zymography...................................... 229
Bahar S. Razavi, Duyen Hoang, and Takov Kuzyakov
23 Multiplex Cathepsin Zymography to Detect Amounts
of Active Cathepsins K, L, S, and V................................. 239
Manu O. Piatt
24 Transfer Zymography........................................... 253
Daniel Pan, Karl A. Wilson, and Anna Tan-Wilson
25 Sequential Detection of Thermophilic Lipase and Protease by Zymography. . . 271
Liliana Kurz, Zully Herndndez, Lellys M. Contreras, and Jeff Wilkesman
Contents xv
26 Calpain Zymography: General Methodology and Protocol............... 279
Kevin K. W. Wang
27 CTAB Zymography for the Analysis of Aspartic Proteases
from Marine Sponges........................................... 287
Oscar Gonzdlez and Jeff Wilkesman
28 Zymography Detection of a Bacterial Extracellular Thermoalkaline
Esterase/Lipase Activity......................................... 295
Maria Tapizquent, Marleny Ferndndez, Georgina Barreto,
Zully Herndndez, Lellys M. Contreras, Liliana Kurz, and Jeff Wilkesman
29 Amylase Zymography........................................... 301
Adarelys Andrades and Lellys M. Contreras
Index........................................................... 309
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| isbn | 9781493971091 |
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| spelling | Zymography methods and protocols edited by Jeff Wilkesman, Liliana Kurz New York NY Humana Press [2017] © 2017 xx, 310 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Springer protocols Methods in molecular biology 1626 Wilkesman, Jeff edt Kurz, Liliana edt Erscheint auch als Online-Ausgabe, eBook Zymography New York, NY : Humana Press, 2017 Online-Ressource (XX, 310 p. 67 illus., 45 illus. in color, online resource) 978-1-4939-7111-4 Methods in molecular biology 1626 (DE-604)BV035362695 1626 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029836629&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zymography methods and protocols Methods in molecular biology |
| title | Zymography methods and protocols |
| title_auth | Zymography methods and protocols |
| title_exact_search | Zymography methods and protocols |
| title_full | Zymography methods and protocols edited by Jeff Wilkesman, Liliana Kurz |
| title_fullStr | Zymography methods and protocols edited by Jeff Wilkesman, Liliana Kurz |
| title_full_unstemmed | Zymography methods and protocols edited by Jeff Wilkesman, Liliana Kurz |
| title_short | Zymography |
| title_sort | zymography methods and protocols |
| title_sub | methods and protocols |
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