Optical tweezers: methods and protocols
Introduction to optical tweezers -- Exact theory of optical tweezers and its application to absolute calibration -- Beyond the hookean spring model: Direct measurement of optical forces through light momentum changes -- A surface-coupled optical trap with 1-bp precision via active stabilization -- I...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
[2017]
|
| Schriftenreihe: | Methods in molecular biology
1486 |
| Schlagworte: | |
| Zusammenfassung: | Introduction to optical tweezers -- Exact theory of optical tweezers and its application to absolute calibration -- Beyond the hookean spring model: Direct measurement of optical forces through light momentum changes -- A surface-coupled optical trap with 1-bp precision via active stabilization -- Implementation and tuning of an optical tweezers force-clamp feedback system -- Custom-made microspheres for optical tweezers -- Optical torque wrench design and calibration -- High-resolution "fleezers": Dual-trap optical tweezers combined with single-molecule fluorescence detection -- Versatile quadruple-trap optical tweezers for dual DNA experiments -- Probing DNA-DNA interactions with a combination of quadruple-trap optical tweezers and microfluidics -- Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement -- Mechanically watching the clpXP proteolytic machinery -- Deciphering the molecular mechanism of the bacteriophage phi29 DNA packaging motor -- Single-molecule protein folding experiments using high-precision optical tweezers -- Observing single RNA polymerase molecules down to base-pair resolution -- Optical tweezers-based measurements of forces and dynamics at microtubule ends -- Simultaneous manipulation and super-resolution fluorescence imaging of individual kinetochores coupled to microtubule tips -- Measurement of force-dependent release rates of cytoskeletal motors -- Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers -- Quantifying force and viscoelasticity inside living cells using an active-passive calibrated optical trap -- Measuring molecular forces using calibrated optical tweezers in living cells |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | xii, 555 Seiten Illustrationen, Diagramme 25.4 cm x 17.8 cm, 0 g |
| ISBN: | 9781493964192 1493964194 |
Internformat
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| 490 | 1 | |a Methods in molecular biology |v 1486 | |
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Datensatz im Suchindex
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| author2 | Gennerich, Arne |
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| building | Verbundindex |
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| classification_rvk | WC 2500 |
| ctrlnum | (OCoLC)965541565 (DE-599)DNB1100301283 |
| discipline | Biologie |
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| id | DE-604.BV044410561 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T07:52:13Z |
| institution | BVB |
| isbn | 9781493964192 1493964194 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-029812369 |
| oclc_num | 965541565 |
| open_access_boolean | |
| owner | DE-703 |
| owner_facet | DE-703 |
| physical | xii, 555 Seiten Illustrationen, Diagramme 25.4 cm x 17.8 cm, 0 g |
| publishDate | 2017 |
| publishDateSearch | 2017 |
| publishDateSort | 2017 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Optical tweezers methods and protocols edited by Arne Gennerich New York Humana Press [2017] xii, 555 Seiten Illustrationen, Diagramme 25.4 cm x 17.8 cm, 0 g txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1486 Springer protocols Includes bibliographical references and index Introduction to optical tweezers -- Exact theory of optical tweezers and its application to absolute calibration -- Beyond the hookean spring model: Direct measurement of optical forces through light momentum changes -- A surface-coupled optical trap with 1-bp precision via active stabilization -- Implementation and tuning of an optical tweezers force-clamp feedback system -- Custom-made microspheres for optical tweezers -- Optical torque wrench design and calibration -- High-resolution "fleezers": Dual-trap optical tweezers combined with single-molecule fluorescence detection -- Versatile quadruple-trap optical tweezers for dual DNA experiments -- Probing DNA-DNA interactions with a combination of quadruple-trap optical tweezers and microfluidics -- Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement -- Mechanically watching the clpXP proteolytic machinery -- Deciphering the molecular mechanism of the bacteriophage phi29 DNA packaging motor -- Single-molecule protein folding experiments using high-precision optical tweezers -- Observing single RNA polymerase molecules down to base-pair resolution -- Optical tweezers-based measurements of forces and dynamics at microtubule ends -- Simultaneous manipulation and super-resolution fluorescence imaging of individual kinetochores coupled to microtubule tips -- Measurement of force-dependent release rates of cytoskeletal motors -- Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers -- Quantifying force and viscoelasticity inside living cells using an active-passive calibrated optical trap -- Measuring molecular forces using calibrated optical tweezers in living cells Optical tweezersvLaboratory manuals Laser (DE-588)4034610-9 gnd rswk-swf Teilchenfalle (DE-588)4349942-9 gnd rswk-swf Laser (DE-588)4034610-9 s Teilchenfalle (DE-588)4349942-9 s DE-604 Gennerich, Arne edt Erscheint auch als Online-Ausgabe, eBook 978-1-4939-6421-5 Methods in molecular biology 1486 (DE-604)BV035362695 1486 |
| spellingShingle | Optical tweezers methods and protocols Methods in molecular biology Optical tweezersvLaboratory manuals Laser (DE-588)4034610-9 gnd Teilchenfalle (DE-588)4349942-9 gnd |
| subject_GND | (DE-588)4034610-9 (DE-588)4349942-9 |
| title | Optical tweezers methods and protocols |
| title_auth | Optical tweezers methods and protocols |
| title_exact_search | Optical tweezers methods and protocols |
| title_full | Optical tweezers methods and protocols edited by Arne Gennerich |
| title_fullStr | Optical tweezers methods and protocols edited by Arne Gennerich |
| title_full_unstemmed | Optical tweezers methods and protocols edited by Arne Gennerich |
| title_short | Optical tweezers |
| title_sort | optical tweezers methods and protocols |
| title_sub | methods and protocols |
| topic | Optical tweezersvLaboratory manuals Laser (DE-588)4034610-9 gnd Teilchenfalle (DE-588)4349942-9 gnd |
| topic_facet | Optical tweezersvLaboratory manuals Laser Teilchenfalle |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT gennericharne opticaltweezersmethodsandprotocols |