Methods in cell biology, Volume 40, A practical guide to the study of calcium in living cells:
Gespeichert in:
Bibliographische Detailangaben
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: San Diego Academic Press ©1994
Schriftenreihe:Methods in cell biology v. 40
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Online-Zugang:FAW01
URL des Erstveröffentlichers
Beschreibung:Includes bibliographical references and index
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Key Features * Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes * Fluorescent dyes * Luminescent proteins * Includes techniques for perturbing intracellular Ca2+ * Covers detailed methodology plus problems and pitfalls of each technique * Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program * Color plates illustrate techniques such as * Confocal ratio-imaging * Use of aequorin
Beschreibung:xiii, 368 pages
ISBN:0125641419
9780125641418
0125228104

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