Heterologous protein production in CHO cells: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2017]
|
| Schriftenreihe: | Methods in molecular biology
1603 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | x, 252 Seiten Illustrationen, Diagramme |
| ISBN: | 9781493969715 1493969714 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804177589720317952 |
|---|---|
| adam_text | Titel: Heterologous protein production in CHO cells
Autor: Meleady, Paula
Jahr: 2017
Contents
Preface.......................................................... p
Contributors.......................................................... ix
1 Strategies and Considerations for Improving Expression of Difficult
to Express Proteins in CHO Cells................................. 1
Christina S. Alves and Terrence M. Dobrowsky
2 Glycoengineering of CHO Cells to Improve Product Quality............. 25
Qiong Wans, Bojiao Tin, Cheng-Yu Chung, and Michael J. Betenbaugh
3 Large-Scale Transient Transfection of Chinese Hamster Ovary Cells
in Suspension................................................. 45
Yashas Rajendra, Sowmya Balasubramanian, and David L. Hacker
4 Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR
for Evaluation of Antibody Expression in Transient Gene Expression........ 57
Patrick Mayrhofer and Renate Kunert
5 Anti-Apoptosis Engineering for Improved Protein Production
from CHO Cells............................................... 71
Eric Baek, Soo Min Noh, and Gyun Min Lee
6 Conditional Knockdown of Endogenous MicroRNAs in CHO Cells
Using TET-ON-S^wD/Sponge Vectors............................. 87
Alan Costello, Nga Lao, Martin Clynes, and Niall Barron
7 Application of CRISPR/Cas9 Genome Editing to Improve
Recombinant Protein Production in CHO Cells....................... 101
Lise Marie Grav, Karen Julie la Cour Karottki, Joe Seong Lee,
and Helene Faustrup Kildegaard
8 Improved CHO Cell Line Stability and Recombinant Protein Expression
During Long-Term Culture...................................... 119
Zeynep Betts and Alan J. Dickson
9 Selection of High-Producing Clones Using FACS for CHO
Cell Line Development.......................................... 143
Clair Gallagher and Paul S. Kelly
10 The Omics Revolution in CHO Biology: Roadmap to Improved
CHO Productivity............................................. 153
Hussain Dahodwala and Susan T. Sharfstein
11 A Bioinformatics Pipeline for the Identification of CHO Cell Differential
Gene Expression from RNA-Seq Data............................... 169
Craig Monger, Krishna Motheramgari, John McSharry, Niall Barron,
and Colin Clarke
vii
viii
Contents
12 Filter-Aided Sample Preparation (FASP) for Improved Proteome
Analysis of Recombinant Chinese Hamster Ovary Cells.................. 187
Orla Coleman, Michael Henry, Martin Clynes, and Paula Meleady
13 Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the
Phosphoproteome of Recombinant Chinese Hamster Ovary Cells.......... 195
Michael Henry, Orla Coleman, Prashant, Martin Clynes,
and Paula Meleady
14 Engineer Medium and Feed for Modulating N-Glycosylation
of Recombinant Protein Production in CHO Cell Culture............... 209
Tuzhou Fan, Helene Faustrup Kildeßaard, and Mikael Rordam Andersen
15 Glycosylation Analysis of Therapeutic Glycoproteins Produced
in CHO Cells................................................. 227
Sara Carillo, Stefan Mittermayr, Amy Farrell, Simone Albrecht,
and Jonathan Bones
16 Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses . . . 243
Catherine E.M. Hoßwood, Lesley M. Chiverton, and C. Mark Smales
Index............................................................... 251
|
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| indexdate | 2024-07-10T07:50:29Z |
| institution | BVB |
| isbn | 9781493969715 1493969714 |
| language | English |
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| publishDate | 2017 |
| publishDateSearch | 2017 |
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| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Springer protocols Methods in molecular biology |
| spelling | Heterologous protein production in CHO cells methods and protocols edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland New York, NY Humana Press [2017] © 2017 x, 252 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Springer protocols Methods in molecular biology 1603 Meleady, Paula (DE-588)1133299180 edt Erscheint auch als Online-Ausgabe, eBook 978-1-4939-6972-2 Methods in molecular biology 1603 (DE-604)BV035362695 1603 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029752648&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Heterologous protein production in CHO cells methods and protocols Methods in molecular biology |
| title | Heterologous protein production in CHO cells methods and protocols |
| title_auth | Heterologous protein production in CHO cells methods and protocols |
| title_exact_search | Heterologous protein production in CHO cells methods and protocols |
| title_full | Heterologous protein production in CHO cells methods and protocols edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland |
| title_fullStr | Heterologous protein production in CHO cells methods and protocols edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland |
| title_full_unstemmed | Heterologous protein production in CHO cells methods and protocols edited by Paula Meleady, National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland |
| title_short | Heterologous protein production in CHO cells |
| title_sort | heterologous protein production in cho cells methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029752648&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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