Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification: = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Würzburg
2016
|
| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | xvi, 221 Seiten Illustrationen, Diagramme 30 cm |
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| 100 | 1 | |a Stuckensen, Kai |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification |b = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung |c vorgelegt von Dipl.-Ing. Kai Stuckensen aus Würzburg |
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Record in the Search Index
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| adam_text |
FABRICATION OF HIERARCHICAL CELL CARRIER
MATRICES FOR TISSUE REGENERATION BY
DIRECTIONAL SOLIDIFICATION
DISSERTATION ZUR ERLANGUNG DES
NATURWISSENSCHAFTLICHEN DOKTORGRADES
DER JULIUS-MAXIMILIANS-UNIVERSITAET WUERZBURG
VORGELEGT VON
DIPL.-ING. KAI STUCKENSEN
AUS WUERZBURG
WUERZBURG 2016
CONTENTS
1 INTRODUCTION
.
1
2 STATE OF THE
ART.
4
2.1 BONE AND CALCIUM PHOSPHATES
.
4
2.1.1
BRUSHITE.8
2.1.2 HYDROXYAPATITE
.
10
2.2 COLLAGENS AND CARTILAGE
.
12
2.2.1 OSTEOCHONDRAL
TISSUE.15
2.2.2 MENISCUS
TISSUE.17
2.3 TISSUE ENGINEERING OF BONE AND
CARTILAGE.20
2.4 CROSS LINKING OF
COLLAGENS.29
2.5 SOLIDIFICATION
TECHNOLOGIES.32
2.5.1 SOLIDIFICATION OF W
ATER.33
2.5.2 SUPERCOOLING AND SOLIDIFICATION MORPHOLOGIES
.
34
2.5.3 ISSUES RELATED TO MATHEMATICAL DESCRIPTIONS OF SOLIDIFICATION
PROCESSES.
39
2.5.4 DIRECTIONAL SOLIDIFICATION IN BIOMEDICAL
APPLICATIONS.40
3 MATERIALS AND
METHODS.
43
3.1 A
LGINATE.
43
3.2 CALCIUM
PHOSPHATES.43
3.2.1 SYNTHESIS OF
BRUSHITE.43
3.2.2 SYNTHESIS OF HYDROXYAPATITE
.
.
44
3.2.3 3D POWDER PRINTING OF CALCIUM
PHOSPHATES.44
3.3 COLLAGEN
.
.
.
45
3.3.1 COLLAGEN
ISOLATION.
46
3.3.2 LYOPHILISATION
.
49
3.3.3 CRYOGENIC FRACTURING
.
49
3.4 DESIGN AND MANUFACTURING OF CUSTOM
DEVICES.50
3.5 SCAFFOLD
CHARACTERIZATION.
51
3.5.1 CRYOSTATIC
SECTIONING.51
3.5.2 SCANNING ELECTRON MICROSCOPY (SEM) AND ENERGY DISPERSIVE X-RAY
SPECTROSCOPY
(EDX).51
3.5.3 MECHANICAL
TESTING.52
3.5.4 MICRO COMPUTER TOMOGRAPHY (P -C T
).52
3.5.5 DEGRADATION
STUDY.53
3.5.6 FOURIER TRANSFORM - INFRARED SPECTROSCOPY
(FT-IR).53
3.5.7 RHEOLOGICAL
MEASUREMENTS.54
3.5.8 ELECTROSTATIC SPINNING OF POLYMERIC SOLUTIONS
.
54
3.5.9 BMP-2 RELEASE
STUDY.
54
3.5.1 OX-RAY DIFFRACTION (X R D
).56
4 RESULTS AND DISCUSSION
.
57
4.1 PROCESS DEVELOPMENT - DIRECTIONAL
SOLIDIFICATION.57
4.1.1 DEVELOPMENT AND ASSEMBLY OF AN ADJUSTABLE CRYOSTRUCTURING
DEVICE. 59
4.1.2 PROCESS EVALUATION BY FABRICATION OF CRYOSTRUCTURED ALGINATE
SCAFFOLDS.
64
4.1.3 OPTIMIZATION OF PROCESSING
STEPS.73
4.2 PRECURSOR MATERIALS AND PROCESSING INDUCED ALTERATIONS
.
95
4.2.1
COLLAGENS.95
4.2.2 CALCIUM
PHOSPHATES.100
4.3 BONE
SCAFFOLDS.
104
4.4 MENISCUS
SCAFFOLDS.115
4.4.1 ICE CRYSTAL GROWTH ANGLED TO EXTERNAL TEMPERATURE GRADIENT
.
115
4.4.2 CRYOSTRUCTURED MENISCUS
SCAFFOLDS.117
4.5 OSTEOCHONDRAL
SCAFFOLDS.
128
4.5.1 STRATEGY FOR OSTEOCHONDRAL TISSUE ENGINEERING
.
128
4.5.2 3D POWDER PRINTING OF CALCIUM
PHOSPHATES.129
4.5.3 OSTEOCHONDRAL
SCAFFOLDS.131
4.5.4 PREPARATION OF ARTICULAR CARTILAGE SURFACE BY ELECTROSTATIC
SPINNING. 142
4.6 DEVELOPMENT OF MECHANICAL TESTING PROCEDURE IN CONFINED
COMPRESSION.
143
4.6.1 EXPERIMENTAL
SETUP.
144
4.6.2 RESULTS OF DEVELOPED TESTING METHOD
.
149
4.7 BIOLOGICAL
RESULTS.155
4.7.1 BONE
SCAFFOLDS.
156
4.7.2 CARTILAGE
SCAFFOLDS.161
4.8 OUTLOOK ON FURTHER APPLICATIONS
.
.
166
4.8.1 VARIATION OF PRECURSOR M
ATERIALS.
166
4.8.2 INTERVERTEBRAL DISC SCAFFOLDS. 167
4.8.3 CELL CULTURE UNDER MECHANICAL
STIMULATION.168
4.8.4 INFLUENCE OF CELLULAR BEHAVIOUR BY TUNING OF TOPOGRAPHICAL SURFACE
STRUCTURE.
168
4.8.5
ELECTROSPINNING.169
4.8.6 FILTER
MATERIALS.169
4.8.7 MATERIAL FOR MULTISTAGE CATALYSIS IN CONTINUOUS CHEMICAL
REACTIONS. 170
5 CONCLUSION
.
172
5.1 DEVELOPMENT OF A DIRECTIONAL SOLIDIFICATION PROCESS AND DEVICE
.
172
5.2 PRECURSOR MATERIALS AND PROCESSING INDUCED ALTERATIONS
.
173
5.3 EVALUATION AND OPTIMIZATION OF THE CRYOSTRUCTURING PROCESS
.
174
5.4 CRYOSTRUCTURED
SCAFFOLDS.
178
5.5 DEVELOPMENT OF MECHANICAL TESTING IN CONFINED COMPRESSION
.
184
5.6 BIOLOGICAL SCAFFOLD PERFORMANCE
.
187
6 SUMMARY
.
189
7 ZUSAMMENFASSUNG
.
193
8 ACKNOWLEDGMENT
.
198
9
REFERENCES.
201
10
APPENDIX.
221 |
| any_adam_object | 1 |
| author | Stuckensen, Kai |
| author_facet | Stuckensen, Kai |
| author_role | aut |
| author_sort | Stuckensen, Kai |
| author_variant | k s ks |
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| dewey-full | 670 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 670 - Manufacturing |
| dewey-raw | 670 |
| dewey-search | 670 |
| dewey-sort | 3670 |
| dewey-tens | 670 - Manufacturing |
| discipline | Chemie / Pharmazie Werkstoffwissenschaften / Fertigungstechnik |
| format | Thesis Book |
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| spelling | Stuckensen, Kai Verfasser aut Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung vorgelegt von Dipl.-Ing. Kai Stuckensen aus Würzburg Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung Würzburg 2016 xvi, 221 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Julius-Maximilians-Universität Würzburg 2017 Zusammenfassung in deutscher und englischer Sprache Knorpel (DE-588)4031355-4 gnd rswk-swf Gerichtete Erstarrung (DE-588)4456229-9 gnd rswk-swf Tissue Engineering (DE-588)4646061-5 gnd rswk-swf Knochen (DE-588)4128031-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Tissue Engineering (DE-588)4646061-5 s Knochen (DE-588)4128031-3 s Knorpel (DE-588)4031355-4 s Gerichtete Erstarrung (DE-588)4456229-9 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-145510 Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification Würzburg : Universität Würzburg, 2017 Online-Ressource https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-145510 Resolving-System kostenfrei Volltext B:DE-101 application/pdf http://d-nb.info/1132730740/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029736302&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Stuckensen, Kai Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung Knorpel (DE-588)4031355-4 gnd Gerichtete Erstarrung (DE-588)4456229-9 gnd Tissue Engineering (DE-588)4646061-5 gnd Knochen (DE-588)4128031-3 gnd |
| subject_GND | (DE-588)4031355-4 (DE-588)4456229-9 (DE-588)4646061-5 (DE-588)4128031-3 (DE-588)4113937-9 |
| title | Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung |
| title_alt | Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung |
| title_auth | Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung |
| title_exact_search | Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung |
| title_full | Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung vorgelegt von Dipl.-Ing. Kai Stuckensen aus Würzburg |
| title_fullStr | Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung vorgelegt von Dipl.-Ing. Kai Stuckensen aus Würzburg |
| title_full_unstemmed | Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung vorgelegt von Dipl.-Ing. Kai Stuckensen aus Würzburg |
| title_short | Fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification |
| title_sort | fabrication of hierarchical cell carrier matrices for tissue regeneration by directional solidification herstellung hierarchischer zelltrager matrices zur geweberegeneration mittels gerichteter erstarrung |
| title_sub | = Herstellung hierarchischer Zellträger-Matrices zur Geweberegeneration mittels gerichteter Erstarrung |
| topic | Knorpel (DE-588)4031355-4 gnd Gerichtete Erstarrung (DE-588)4456229-9 gnd Tissue Engineering (DE-588)4646061-5 gnd Knochen (DE-588)4128031-3 gnd |
| topic_facet | Knorpel Gerichtete Erstarrung Tissue Engineering Knochen Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-145510 http://d-nb.info/1132730740/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029736302&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT stuckensenkai fabricationofhierarchicalcellcarriermatricesfortissueregenerationbydirectionalsolidificationherstellunghierarchischerzelltragermatriceszurgeweberegenerationmittelsgerichtetererstarrung AT stuckensenkai herstellunghierarchischerzelltragermatriceszurgeweberegenerationmittelsgerichtetererstarrung |