Cell polarity and morphogenesis:
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Cambridge, MA
Academic Press, an imprint of Elsevier
2017
|
| Edition: | First edition |
| Series: | Methods in cell biology
Volume 139 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | xi, 223 Seiten Illustrationen |
| ISBN: | 9780128093733 |
Staff View
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| 245 | 1 | 0 | |a Cell polarity and morphogenesis |c edited by Thomas Lecuit |
| 250 | |a First edition | ||
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| adam_text | Contents
Contributors.............................................................ix
CHAPTER 1 A user’s Guide for Characterizing Plasma
Membrane Subdomains in Living Cells by Spot
Variation Fluorescence Correlation Spectroscopy..........1
S. Mailfert, Y. Hamon, N. Bertaux, H.-T. He, 0. Marguet
Introduction..............................................2
1. Building a Sensitive and Robust Homemade Spot
Variation Fluorescence Correlation Spectroscopy/Fluorescence
Recovery After Photobleaching Setup.......................4
2. Sample Preparations and Labeling..........................7
3. Fluorescence Correlation Spectroscopy Recording..........11
4. Data Analysis and Interpretation.........................14
Conclusion............................................. 19
Acknowledgments..........................................20
References...............................................20
CHAPTER 2 Investigating Symmetry Breaking in Yeast:
From Seeing to Understanding.............................23
P. Singh, A. Das, R. Li
Introduction.............................................24
1. Genetic Tools............................................27
2. Microscopy ֊Based Methods to Visualize Cell Polarization.28
Conclusion and Future Perspective........................40
Notes....................................................41
Acknowledgments..........................................42
References...............................................42
CHAPTER 3 Precise Tracking of the Dynamics of Multiple
Proteins in Endocytic Events.............................51
A. Picco, M. Kaksonen
Introduction.............................................52
1. Rationale................................................53
2. Sample Preparation.......................................54
3. Fluorescence Microscopy..................................55
4. Image Processing....................................... 58
5. Tracking of the Endocytic Spots..........................61
Contents
6. Correction of Dual Channel Trajectory Pair Alignment....63
7. Generation of Average Trajectories and Alignment of
the Average Trajectories.................................65
Conclusion...............................................67
References...............................................68
CHAPTER 4 Quantitative Approaches for the Study of
Microtubule Aster Motion in Large Eggs...............69
H. Tanimoto, N. Mine
Introduction.............................................70
1. Three-Dimensional Tracking of Microtubule Asters in
Sea Urchin Embryos......................................71
2. Laser Ablation of Astral Microtubules and In Situ
Immunostaining..........................................76
Conclusions..............................................79
Acknowledgments..........................................79
References...............................................79
CHAPTER 5 Dissection and Characterization of Microtubule
Bundles in the Mitotic Spindle Using Femtosecond
Laser Ablation..................................... ....81
R. Buda, K. Vukusic, I.M. Tolič
Introduction.............................................82
1. Sample Preparation......................................84
2. Imaging.................................................85
3. Laser Ablation........................................ 90
4. Image Analysis..........................................93
Conclusion...............................................98
Acknowledgments..........................................98
References...............................................98
CHAPTER 6 Fluorescence exclusion Measurement of
Volume in Live Cells....................................103
C. Cadart, E. Zlotek-Zlotkiewicz, L. Venkova,
0. Thouvenin, V. Racine, M. Le Berre, S. Monnier, M. Piel
Introduction............................................104
1. Method.................................................105
2. FXm Pitfalls and Limitations...........................117
Contents
3. Discussion.............................................117
References........................................... 119
CHAPTER 7 Quantitative Methods to Study Epithelial
Morphogenesis and Polarity.............................I2t
B. Aigouy, C. Collinet, M. Merkel, A. Sagner
Introduction...........................................122
1. Biological Toolbox....................................124
2. Imaging Toolbox........................................127
3. Image Processing Toolbox...............................132
4. Measurements..........................................138
Concluding Remarks.....................................146
Acknowledgments........................................146
References.............................................146
CHAPTER 8 Probing Tissue Interaction with Laser-based
Cauterization in the Early Developing Drosophila
Embryo.................................................153
M. Rauzi
Introduction...........................................154
1. Materials..............................................154
2. Methods................................................156
Conclusion.............................................164
Acknowledgments........................................164
References.............................................165
CHAPTER 9 Optogenetic Inhibition of Apical Constriction
During Drosophila Embryonic Development................167
6. Guglielmi, S. De Renzis
Introduction...........................................168
1. Methods................................................171
Conclusion.............................................183
Acknowledgments........................................184
References.............................................184
viii Contents
CHAPTER 10 Micropipette Aspiration: a Unique Tool
for Exploring Cell and Tissue Mechanics
in vivo............................................,....187
K. Guevorkian, J.-L. Maître
Introduction........................................... 188
1. Building and Preparing a Microaspiration Setup......... 189
2. Subcellular Surface Tension Mapping.....................193
3. Mechanical Properties of Tissues........................196
General Conclusions......................................199
Supplementary Data...................................... 199
References...............................................200
CHAPTER 11 In-vivo Analysis of Morphogenesis in Plants................203
T. Stanislas, 0. Hamant, J. Traas
Introduction.............................................204
1. Measuring the Growth: Live Imaging of Growing
Tissues at Cellular Resolution...........................205
2. Probing the Wall Stiffness and Anisotropy...............214
Conclusions and Perspectives: Beyond Quantitative
Live Imaging......................................... 217
Acknowledgments........................................ 220
References...............................................220
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| series | Methods in cell biology |
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| spelling | Cell polarity and morphogenesis edited by Thomas Lecuit First edition Cambridge, MA Academic Press, an imprint of Elsevier 2017 xi, 223 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 139 Includes bibliographical references and index Zelle (DE-588)4067537-3 gnd rswk-swf Morphogenese (DE-588)4132460-2 gnd rswk-swf Polarität (DE-588)4126483-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zelle (DE-588)4067537-3 s Polarität (DE-588)4126483-6 s Morphogenese (DE-588)4132460-2 s b DE-604 Lecuit, Thomas edt Methods in cell biology Volume 139 (DE-604)BV002534878 139 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029685879&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cell polarity and morphogenesis Methods in cell biology Zelle (DE-588)4067537-3 gnd Morphogenese (DE-588)4132460-2 gnd Polarität (DE-588)4126483-6 gnd |
| subject_GND | (DE-588)4067537-3 (DE-588)4132460-2 (DE-588)4126483-6 (DE-588)4143413-4 |
| title | Cell polarity and morphogenesis |
| title_auth | Cell polarity and morphogenesis |
| title_exact_search | Cell polarity and morphogenesis |
| title_full | Cell polarity and morphogenesis edited by Thomas Lecuit |
| title_fullStr | Cell polarity and morphogenesis edited by Thomas Lecuit |
| title_full_unstemmed | Cell polarity and morphogenesis edited by Thomas Lecuit |
| title_short | Cell polarity and morphogenesis |
| title_sort | cell polarity and morphogenesis |
| topic | Zelle (DE-588)4067537-3 gnd Morphogenese (DE-588)4132460-2 gnd Polarität (DE-588)4126483-6 gnd |
| topic_facet | Zelle Morphogenese Polarität Aufsatzsammlung |
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