Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Dresden
TUDpress
2015
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| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | X, 137 Seiten Illustrationen, Diagramme 21 cm |
| ISBN: | 9783959080125 |
Internformat
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Datensatz im Suchindex
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CONTENTS
1 INTRODUCTION 1
1.1 FLUORESCENCE CONFOCAL MICROSCOPY AS AN IMAGING TOOL 1
1.2 RESEARCH OBJECTIVE AND DISSERTATION OVERVIEW 3
2 BACKGROUND: FLUORESCENCE CONFOCAL LASER-SCANNING MICROSCOPY 5
2.1 FUNDAMENTALS OF LASER-SCANNING MICROSCOPY 5
2.2 PROMISING F-LSM APPLICATIONS IN DERMATOLOGY 11
2.3 IMPACT OF FLUORESCENT DYES ON MICROSCOPY 14
2.3.1 FLUORESCENCE CONTRAST AGENTS IN MEDICINE 15
2.3.2 INFLUENCE OF FLUORESCEIN ON THE LASER ILLUMINATION 16
2.4 INTRODUCTION TO THE WAVEFRONT ABERRATION THEORY 20
2.5 IMPORTANT FIGURES OF MERIT FOR IMAGE QUALITY EVALUATIONS 24
3 MEMS MIRROR BASED F-LSM DESIGN 27
3.1 MEMS MIRROR BASED IMPLICATIONS ON THE MICROSCOPE SPECIFICATIONS .
27
3.1.1 IMPLICATIONS ON THE ILLUMINATION OPTICS 28
3.1.2 REQUIREMENTS ON THE IMAGING OPTICS AND THE Z-SHIFTER 31
3.1.3 FLUORESCENCE DETECTION PATH 34
3.2 INTERMEDIATE RELAY OPTICS - PERFORMANCE SIMULATIONS WITH ZEMAX .
39
3.3 ANALYSIS OF ASSEMBLY TOLERANCES 43
3.4 DYNAMIC DEFORMATIONS OF THE MEMS MIRROR 47
3.5 IMAGE DISTORTION - CAUSES AND CORRECTION METHODS 49
4 F-LSM SYSTEM REALIZATION 55
4.1 F-LSM DEMONSTRATION SYSTEM LAYOUT 55
4.2 QUANTITATIVE OPTICAL PERFORMANCE TESTING 64
4.3 WAVEFRONT ERROR MEASUREMENT WITH A SHACK-HARTMANN SENSOR 72
4.4 IMAGES OF BIOLOGICAL SAMPLES 80
4.5 SUMMARY 84
5 TRANSFER INTO A HANDHELD MICROSCOPE DESIGN 87
5.1 OPTIMIZED IMAGING OPTICS 87
5.1.1 SCAN LENS DESIGN 89
IX
HTTP://D-NB.INFO/1073213498
CONTENTS
5.1.2 TUBE LENS DESIGN 94
*5.2 MOUNTING AND ASSEMBLY TOLERANCES 98
5.3 SUMMARY AND COMPARISON OF BOTH F-LSM SYSTEMS 104
6 DISCUSSION AND OUTLOOK 107
APPENDIX A: FLOW CHART 111
APPENDIX B: SCAN LENS - TECHNICAL DRAWINGS 113
ABBREVIATIONS 117
LIST OF VARIABLES 119
LIST OF FIGURES 121
LIST OF TABLES 127
BIBLIOGRAPHY 129 |
| any_adam_object | 1 |
| author | Bechtel, Christin 1985- |
| author_GND | (DE-588)1073891712 |
| author_facet | Bechtel, Christin 1985- |
| author_role | aut |
| author_sort | Bechtel, Christin 1985- |
| author_variant | c b cb |
| building | Verbundindex |
| bvnumber | BV044260163 |
| classification_rvk | ZN 3750 |
| ctrlnum | (OCoLC)915369814 (DE-599)DNB1073213498 |
| dewey-full | 681.413 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 681 - Precision instruments and other devices |
| dewey-raw | 681.413 |
| dewey-search | 681.413 |
| dewey-sort | 3681.413 |
| dewey-tens | 680 - Manufacture of products for specific uses |
| discipline | Handwerk und Gewerbe / Verschiedene Technologien Elektrotechnik / Elektronik / Nachrichtentechnik |
| format | Thesis Book |
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| spelling | Bechtel, Christin 1985- Verfasser (DE-588)1073891712 aut Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging Christin Bechtel Dresden TUDpress 2015 X, 137 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Technische Universität Dresden 2014 Geräteentwicklung (DE-588)4267986-2 gnd rswk-swf Laser-Rastermikroskop (DE-588)4195932-2 gnd rswk-swf Spiegel (DE-588)4056203-7 gnd rswk-swf Fluoreszenzmikroskop (DE-588)4328299-4 gnd rswk-swf MEMS (DE-588)4824724-8 gnd rswk-swf Konfokale Mikroskopie (DE-588)4336446-9 gnd rswk-swf Laserscanner (DE-588)4419104-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Konfokale Mikroskopie (DE-588)4336446-9 s Laser-Rastermikroskop (DE-588)4195932-2 s Fluoreszenzmikroskop (DE-588)4328299-4 s Laserscanner (DE-588)4419104-2 s Spiegel (DE-588)4056203-7 s MEMS (DE-588)4824724-8 s Geräteentwicklung (DE-588)4267986-2 s DE-604 TUDpress Verlag der Wissenschaften Dresden GmbH (DE-588)1064209653 pbl X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=5300765&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029665102&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bechtel, Christin 1985- Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging Geräteentwicklung (DE-588)4267986-2 gnd Laser-Rastermikroskop (DE-588)4195932-2 gnd Spiegel (DE-588)4056203-7 gnd Fluoreszenzmikroskop (DE-588)4328299-4 gnd MEMS (DE-588)4824724-8 gnd Konfokale Mikroskopie (DE-588)4336446-9 gnd Laserscanner (DE-588)4419104-2 gnd |
| subject_GND | (DE-588)4267986-2 (DE-588)4195932-2 (DE-588)4056203-7 (DE-588)4328299-4 (DE-588)4824724-8 (DE-588)4336446-9 (DE-588)4419104-2 (DE-588)4113937-9 |
| title | Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging |
| title_auth | Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging |
| title_exact_search | Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging |
| title_full | Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging Christin Bechtel |
| title_fullStr | Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging Christin Bechtel |
| title_full_unstemmed | Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging Christin Bechtel |
| title_short | Development of a MEMS-based confocal laser scanning microscope for fluorescence imaging |
| title_sort | development of a mems based confocal laser scanning microscope for fluorescence imaging |
| topic | Geräteentwicklung (DE-588)4267986-2 gnd Laser-Rastermikroskop (DE-588)4195932-2 gnd Spiegel (DE-588)4056203-7 gnd Fluoreszenzmikroskop (DE-588)4328299-4 gnd MEMS (DE-588)4824724-8 gnd Konfokale Mikroskopie (DE-588)4336446-9 gnd Laserscanner (DE-588)4419104-2 gnd |
| topic_facet | Geräteentwicklung Laser-Rastermikroskop Spiegel Fluoreszenzmikroskop MEMS Konfokale Mikroskopie Laserscanner Hochschulschrift |
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