Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
2016
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 211 Seiten Illustrationen, Diagramme |
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MARC
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| 100 | 1 | |a Grube, Maurice |e Verfasser |0 (DE-588)1132227224 |4 aut | |
| 245 | 1 | 0 | |a Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei |c by Maurice Grube |
| 264 | 1 | |a Berlin |c 2016 | |
| 300 | |a XV, 211 Seiten |b Illustrationen, Diagramme | ||
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| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Freie Universität Berlin |d 2017 | ||
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|---|---|
| adam_text | C
ONTENTS
SUMMARY...................................................................................................................................HI
ZUSAMMENFASSUNG.................................................................................................................
VIII
ABBREVIATIONS........................................................................................................................
XIII
1.
INTRODUCTION.............................................................................................................................1
1.1 PROTOZOAN
PARASITES...........................................................................................................1
1.2 HOST IMMUNE SYSTEM AND PARASITE
INTERACTION..................................................................5
1.3 VARIANT SURFACE GLYCOPROTEIN
(VSG)................................................................................9
1.4 GLYCOSYLPHOSPHATIDYLINOSITOL
(GPI)...............................................................................
15
1.5 CARBOHYDRATE
CHEMISTRY................................................................................................
25
1.6 CHEMICAL SYNTHESIS OF
GPI............................................................................................
26
1.7 SYNTHESIS O F
GPI-CONJUGATES..........................................................................................
32
2. AIM OF THE
THESIS..................................................................................................................
35
3. SYNTHETIC
ANALYSIS................................................................................................................37
3.1 CONJUGATION OF GLYCANS TO VSG
FRAGMENTS....................................................................37
3.2 GPI GLYCAN
SYNTHESIS.......................................................................................................38
4. RESULTS AND DISCUSSION: SYNTHESIS OF GPIS FOR GLYCAN MICROARRAY
.....................................
42
4.1 T ETRASACCHARIDE G
............................................................................................................42
4.2 PENTASACCHARIDE WITH THIOLINKER
45.................................................................................51
4.3 ASSEMBLY OF A PSEUDOHEPTASACCHARIDE WITH
THIOLINKER...................................................53
4.4 ASSEMBLY OF TETRAGALACTOSIDE H
.....................................................................................
56
4.5 TETRAGALACTOSE-MAN I WITH
THIOLINKER..............................................................................60
4.6 MYO-INOSITOL WITH PHOSPHO-THIOLINKER 90
.......................................................................
63
4.7 DISACCHARIDE WITH THIOLINKER
93......................................................................................63
4.7 TRISACCHARIDE WITH THIOLINKER
110...................................................................................66
4.9 PSEUDOPENTASACCHARIDE PHOSPHO-THIO LINKER
120............................................................ 67
4.10 PSEUOHEPTASACCHARIDE PHOSPHO-THIO LINKER
129............................................................ 70
4.11 CONCLUSION AND OUTLOOK: SYNTHESIS O F GPIS FOR GLYCAN MICROARRAY
..........................
72
5. RESULTS AND DISCUSSION: GLYCAN MICRO ARRAY OF SYNTHETIC GPI
STRUCTURES
............................
74
5.1 INVESTIGATION OF T. BRUCEI GPI STRUCTURES
.......................................................................
74
5.2 MICE SERA - INFECTION MODEL T. BRUCEI A
NTATL.LE........................................................ 76
5.3 CONCLUSION: MICE SERA - INFECTION MODEL T. BRUCEI ANTATL.LE
....................................
79
5.4 HUMAN SERA - SAMPLES OF HAT PATIENTS
........................................................................
80
5.5 CONCLUSION AND OUTLOOK: GLYCAN MICROARRAY WITH HUMAN SERA
.....................................
85
5.6 CROSS-REACTIVITY OF ANTI-GPI ANTIBODIES OF EUROPEAN T. GONDII
PATIENTS
.......................
86
5.7 CONCLUSION: CROSS-REACTIVITY OF ANTI-GPI
ANTIBODIES.......................................................89
6. RESULTS AND DISCUSSION: SYNTHESIS OF GPI FRAGMENTS FOR MACROPHAGE
ACTIVATION
...............
90
6.1 MYO-INOSITOL PHOSPHOLIPID
134.......................................................................................90
6.2 PSEUDODISACCHARIDE-PHOSPHOLIPID
137............................................................................91
6.3 DISACCHARIDE
140............................................................................................................91
6.4 TRISACCHARIDE
142............................................................................................................92
6.5 TETRAGALACTOSIDE
144......................................................................................................
93
6.6 TETRASACCHARIDE
148........................................................................................................94
6.7 TRISACCHARIDE
150............................................................................................................94
6.8 PSEUDOHEPTASACCHARIDE
152............................................................................................
95
6.9 CONCLUSION: SYNTHESIS OF GPI FRAGMENTS FOR MACROPHAGE ACTIVATION
.........................
95
7. INVESTIGATION OF MACROPHAGE ACTIVATION BY GPI
FRAGMENTS.................................................96
7.1 CONCLUSION AND OUTLOOK: MACROPHAGE ACTIVATION
.........................................................
98
8. GPI FRAGMENTS FOR A LIGATION
METHOD..................................................................................
99
8.1 STRATEGY 1: FRAGMENT
CONDENSATION...............................................................................
99
8.2 STRATEGY 2: PHOSPHORYLATION OF THE
PEPTIDE..................................................................100
8.3 STRATEGY 3: PHOSPHORYLATION OF THE
GLYCAN....................................................................101
8.4 CONCLUSION AND OUTLOOK: GPI FRAGMENTS FOR A LIGATION METHOD
................................
102
9. EXPERIMENTAL
PART................................................................................................................104
9.1 MATERIALS AND
METHODS..................................................................................................
104
9.2 GENERAL SYNTHETIC
METHODS...........................................................................................105
9.3 PREPARATION OF GLYCAN MICROARRAY
................................................................................
110
9.4 SYNTHETIC PART FOR CHAPTER 4
..........................................................................................I
L L
9.5 SYNTHETIC PART FOR CHAPTER 6
..........................................................................................175
9.6 SYNTHETIC PART FOR CHAPTER 8
..........................................................................................184
10.
REFERENCES.........................................................................................................................
189
11.
ACKNOWLEDGMENTS............................................................................................................
204
12.
APPENDIX...........................................................................................................................205
|
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| indexdate | 2024-07-10T07:47:06Z |
| institution | BVB |
| language | English |
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| physical | XV, 211 Seiten Illustrationen, Diagramme |
| publishDate | 2016 |
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| spelling | Grube, Maurice Verfasser (DE-588)1132227224 aut Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei by Maurice Grube Berlin 2016 XV, 211 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Freie Universität Berlin 2017 Glykosylphosphatidylinosit-Anker (DE-588)4480925-6 gnd rswk-swf Trypanosoma brucei (DE-588)4186350-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Trypanosoma brucei (DE-588)4186350-1 s Glykosylphosphatidylinosit-Anker (DE-588)4480925-6 s DE-188 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029631774&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Grube, Maurice Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei Glykosylphosphatidylinosit-Anker (DE-588)4480925-6 gnd Trypanosoma brucei (DE-588)4186350-1 gnd |
| subject_GND | (DE-588)4480925-6 (DE-588)4186350-1 (DE-588)4113937-9 |
| title | Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei |
| title_auth | Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei |
| title_exact_search | Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei |
| title_full | Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei by Maurice Grube |
| title_fullStr | Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei by Maurice Grube |
| title_full_unstemmed | Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei by Maurice Grube |
| title_short | Synthesis and biological evaluation of Glycosylphosphatidylinositols from Trypanosoma Brucei |
| title_sort | synthesis and biological evaluation of glycosylphosphatidylinositols from trypanosoma brucei |
| topic | Glykosylphosphatidylinosit-Anker (DE-588)4480925-6 gnd Trypanosoma brucei (DE-588)4186350-1 gnd |
| topic_facet | Glykosylphosphatidylinosit-Anker Trypanosoma brucei Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029631774&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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