Synapse development: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY, U.S.A.
Humana Press
[2017]
|
| Schriftenreihe: | Methods in molecular biology
1538 |
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | xi, 372 Seiten Illustrationen 25.4 cm x 17.8 cm |
| ISBN: | 9781493966868 1493966863 |
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| 245 | 1 | 0 | |a Synapse development |b methods and protocols |c edited by Alexandros Poulopoulos, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA |
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Datensatz im Suchindex
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Titel: Synapse development
Autor: Poulopoulos, Alexandros
Jahr: 2017
Contents
Preface. p
Contributors. ix
Part I Forming Synapses in a Dish
1 A Cell Culture System to Investigate the Presynaptic Control
of Subsynaptic Membrane Differentiation at the Neuromuscular Junction. 3
Nadine Schmidt, Sreya Basu, Stephan Kröger, and Hans Rudolf Brenner
2 Co-culture Synaptogenic Assay: A New Look at Fluorescence Reporters
and Technological Devices. 13
Karen Perez de Arce and Massimiliano Stagi
3 Synaptogenic Assays Using Neurons Cultured
on Micropatterned Substrates. 29
Katalin Czöndör and Olivier Thoumine
4 Monitoring Synapses Via Trans-Synaptic GFP Complementation. 45
Theodoros Tsetsenis
5 Generation of Spinal Motor Neurons from Human Pluripotent
Stem Cells. 53
David P. Santos and Evangelos Kiskinis
Part II Analyzing Synaptic Constituents
6 Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins . 69
BekirAltas, Olaf Jahn, andHiroshi Kawabe
7 In Situ Protein Binding Assay Using Fc-Fusion Proteins. 83
Nirmala Padmanabhan and Tabrez J. Siddiqui
8 Reconstitution of Synaptic SNAREs into Large Liposomes
with Reduced Curvature Stress. 93
Javier M. Hernandez
9 Isolation of Synaptosomes, Synaptic Plasma Membranes,
and Synaptic Junctional Complexes. 107
Mary L. Michaelis, Lei Jiang, and Elias K. Michaelis
10 Purification of Synaptosome Populations Using Fluorescence-Activated
Synaptosome Sorting. 121
Elisa Luquet, Christoph Biesemann, Annie Munier, and Etienne Herzog
Part III Observing Synaptic Structure and Ultrastructure
11 Optimized Protocol for Imaging Cleared Neural Tissues
Using Light Microscopy. 137
Toh Isogai, Douglas S. Richardson, Catherine Dulac, and Joseph Bergan
vii
viii
Contents
12 Structured Illumination Microscopy for the Investigation
of Synaptic Structure and Function. 155
Soyon Hong, Daniel K. Wilton, Beth Stevens, and Douglas S. Richardson
13 3D d STORM Imaging of Fixed Brain Tissue. 169
Frank Herrmannsdörfer, Benjamin Flottmann, Siddarth Nanguneri,
Varun Venkataramani, Heinz Horstmann, Thomas Kuner,
and Mike Heilemann
14 Photomarking Relocalization Technique for Correlated Two-Photon
and Electron Microcopy Imaging of Single Stimulated Synapses. 185
Mi que I Bosch, Jorge Castro, Mriganka Sur, and Tasunori Hayashi
15 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice
Culture by High-Pressure Freezing and Electron Tomography. 215
Cordelia Imig and Benjamin H. Cooper
Part IV Following Synapse Dynamics
16 Analyzing Endosomal Docking, Fusion, Sorting, and Budding
Mechanisms in Isolated Organelles. 235
Sina V. Barysch and Ioanna Bethani
17 Concurrent Imaging of Receptor Trafficking and Calcium Dynamics
by Spinning Disk Confocal Microscopy. 249
DeLaine D. Larsen, Regina Wai-Tan Choy, and Minjong Park
18 Imaging Activity-Dependent Signaling Dynamics at the Neuronal Synapse
Using FRET-Based Biosensors. 261
Zohreh Farsi and Andrew Woehler
19 Analyzing Structural Plasticity of Dendritic Spines in Organotypic
Slice Culture. 277
Mathias De Roo and Adema Ribic
Part V Revealing Patterns of Synaptic Connectivity
20 Using Fluorescent Markers to Estimate Synaptic Connectivity In Situ. 293
Mrinalini Hoon, Raunak Sinha, and Haruhisa Okawa
21 Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity . 321
Matthias G. Haberl, Melanie Ginger, and Andreas Frick
22 Mapping Synaptic Inputs of Developing Neurons Using Calcium Imaging. 341
Johan Winnubst and Christian Lohmann
23 Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus . 353
Laura Cocas and Gloria Fernandez
Index. 367 |
| any_adam_object | 1 |
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| dewey-full | 610 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610 |
| dewey-search | 610 |
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| discipline | Biologie Medizin |
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| spelling | Synapse development methods and protocols edited by Alexandros Poulopoulos, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA New York, NY, U.S.A. Humana Press [2017] xi, 372 Seiten Illustrationen 25.4 cm x 17.8 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1538 Springer protocols Entwicklung (DE-588)4113450-3 gnd rswk-swf Synapse (DE-588)4184224-8 gnd rswk-swf Nervenzelle (DE-588)4041649-5 gnd rswk-swf Cell culture Nervous system development Neurons Synapse dynamics Synaptic constituents (DE-588)4143413-4 Aufsatzsammlung gnd-content Synapse (DE-588)4184224-8 s Nervenzelle (DE-588)4041649-5 s Entwicklung (DE-588)4113450-3 s DE-604 Poulopoulos, Alexandros 1980- (DE-588)141933054 edt Springer Science + Business Media LLC (DE-588)1065492340 pbl Erscheint auch als Online-Ausgabe 978-1-4939-6688-2 Methods in molecular biology 1538 (DE-604)BV035362695 1538 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=4497881c42da4d0099e544db6156cb96&prov=M&dok_var=1&dok_ext=htm Inhaltstext HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029628301&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Synapse development methods and protocols Methods in molecular biology Entwicklung (DE-588)4113450-3 gnd Synapse (DE-588)4184224-8 gnd Nervenzelle (DE-588)4041649-5 gnd |
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| title | Synapse development methods and protocols |
| title_auth | Synapse development methods and protocols |
| title_exact_search | Synapse development methods and protocols |
| title_full | Synapse development methods and protocols edited by Alexandros Poulopoulos, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA |
| title_fullStr | Synapse development methods and protocols edited by Alexandros Poulopoulos, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA |
| title_full_unstemmed | Synapse development methods and protocols edited by Alexandros Poulopoulos, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA |
| title_short | Synapse development |
| title_sort | synapse development methods and protocols |
| title_sub | methods and protocols |
| topic | Entwicklung (DE-588)4113450-3 gnd Synapse (DE-588)4184224-8 gnd Nervenzelle (DE-588)4041649-5 gnd |
| topic_facet | Entwicklung Synapse Nervenzelle Aufsatzsammlung |
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