Membrane Protein Purification and Crystallization: A Practical Guide
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Burlington
Elsevier Science
2003
|
| Ausgabe: | 2nd ed |
| Schlagworte: | |
| Zusammenfassung: | This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins |
| Beschreibung: | Description based on publisher supplied metadata and other sources |
| Beschreibung: | 1 online resource (339 pages) |
| ISBN: | 9780080536170 9780123617767 |
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Datensatz im Suchindex
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| discipline | Biologie |
| edition | 2nd ed |
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| spelling | Hunte, Carola Verfasser aut Membrane Protein Purification and Crystallization A Practical Guide 2nd ed Burlington Elsevier Science 2003 © 2003 1 online resource (339 pages) txt rdacontent c rdamedia cr rdacarrier Description based on publisher supplied metadata and other sources This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins Membrane proteins Membrane proteins--Purification Reinigungsverfahren (DE-588)4391913-3 gnd rswk-swf Kristallisation (DE-588)4033215-9 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf Membranproteine (DE-588)4130026-9 s Reinigungsverfahren (DE-588)4391913-3 s 1\p DE-604 Kristallisation (DE-588)4033215-9 s 2\p DE-604 von Jagow, Gebhard Sonstige oth Schagger, Hermann Sonstige oth Erscheint auch als Druck-Ausgabe Hunte, Carola Membrane Protein Purification and Crystallization : A Practical Guide 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Hunte, Carola Membrane Protein Purification and Crystallization A Practical Guide Membrane proteins Membrane proteins--Purification Reinigungsverfahren (DE-588)4391913-3 gnd Kristallisation (DE-588)4033215-9 gnd Membranproteine (DE-588)4130026-9 gnd |
| subject_GND | (DE-588)4391913-3 (DE-588)4033215-9 (DE-588)4130026-9 |
| title | Membrane Protein Purification and Crystallization A Practical Guide |
| title_auth | Membrane Protein Purification and Crystallization A Practical Guide |
| title_exact_search | Membrane Protein Purification and Crystallization A Practical Guide |
| title_full | Membrane Protein Purification and Crystallization A Practical Guide |
| title_fullStr | Membrane Protein Purification and Crystallization A Practical Guide |
| title_full_unstemmed | Membrane Protein Purification and Crystallization A Practical Guide |
| title_short | Membrane Protein Purification and Crystallization |
| title_sort | membrane protein purification and crystallization a practical guide |
| title_sub | A Practical Guide |
| topic | Membrane proteins Membrane proteins--Purification Reinigungsverfahren (DE-588)4391913-3 gnd Kristallisation (DE-588)4033215-9 gnd Membranproteine (DE-588)4130026-9 gnd |
| topic_facet | Membrane proteins Membrane proteins--Purification Reinigungsverfahren Kristallisation Membranproteine |
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