Psychoanalysis, trauma and community: history and contemporary reappraisals
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
London and New York
Routledge
2017
|
| Schriftenreihe: | Relational perspectives book series
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XX, 262 Seiten |
| ISBN: | 9781138928220 9781138928237 |
Internformat
MARC
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| 337 | |b n |2 rdamedia | ||
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| 490 | 0 | |a Relational perspectives book series | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Psychologie | |
| 650 | 4 | |a Psychotherapie | |
| 650 | 4 | |a Community psychology | |
| 650 | 4 | |a Psychic trauma |x Treatment | |
| 650 | 4 | |a Disasters |x Psychological aspects | |
| 650 | 4 | |a Psychotherapy | |
| 650 | 4 | |a Community mental health services | |
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| 700 | 1 | |a Goren, Elizabeth R. |d 1949- |0 (DE-588)1122863934 |4 edt | |
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Datensatz im Suchindex
| _version_ | 1804176900785963008 |
|---|---|
| adam_text | THE ROLE OF RBP10, A KEY POST-TRANSCRIPTIONAL REGULATOR IN THE
DEVELOPMENT OF
TRYPANOSOMA BRUCEI
REFEREES: PROF. DR. CHRISTINE CLAYTON (ZMBH)
PROF. DR. LUISE KRAUTH-SIEGEL (BZH)
TABLE OF CONTENTS
ACKNOWLEDGEMENTS
.........................................................
ILL
SUMMARY..................... 6
ZUSAMMENFASSUNG
..........................................................
7
1 INTRODUCTION
.......................................
1.1 BACKGROUND.......
........................
1.2 DISEASES CAUSED BY
T. BRUCEI.
1.3
T. BRUCEI
CELL
STRUCTURE.......................................................................................
10
1.4
T. BRUCEI
LIFE C Y C LE
..............................................................................................
11
1.5 GENE REGULATION IN
TRYPANOSOMES...................................................................14
1.5.1
TRANSCRIPTION.....................................................................................................
14
1.5.2 POST TRANSCRIPTIONAL REGULATION IN
TRYPANOSOMATIDS......................................15
1.6 CONTROL OF TRYPANOSOME
DIFFERENTIATION.........................................................17
1.6.1 BLOODSTREAM TO STUMPY FORMS
TRANSITION........................................................18
1.6.2 STUMPY TO PROCYCLIC FORMS
TRANSITION.............................................................
19
1.7 RBPS REGULATING DEVELOPMENTAL CHANGES IN
T. BRUCEI
..............................
21
1.8 AIM OF THE S TU D Y
...................................................................................................23
2 MATERIALS AND
METHODS...............................................................
24
2.1 TRYPANOSOME CELL CULTURE AND
MANIPULATION.................................................24
2.1.1 BLOODSTREAM FORM
CULTURE................................................................................24
2.1.2 PROCYCLIC FORM
CULTURE....................................................... 25
2.1.3 TRANSFECTION OF BLOODSTREAM/PROCYCLIC TRYPANOSOMES
................................
25
2.1.4 CLONING AND PLASMIDS CONSTRUCTS
..................................................................
26
2.1.5 STABLE CELL LINES FOR INDUCIBLE EXPRESSION
.
....................................................
26
2.1.6 ENDOGENOUS TAGGING OF
RBP10......................................................................27
2.2 TANDEM AFFINITY PURIFICATION (TA P)
............................
27
2.3 YEAST TWO-HYBRID SCREEN.............................................
28
2.3.1 YEAST
TRANSFORMATION......................................................................................
29
2.3.2 GENOME WIDE YEAST TWO-HYBRID SCREEN AND BIOINFORMATICS ANALYSIS
.......
29
2.4 CROSS-LINKING AND RNA
IMMUNOPRECIPITATION...............................................30
2.5 POLYSOME FRACTIONATION AND RNA
SEQ.............................................................31
2.5.1 HIGH THROUGHPUT RNA SEQUENCING AND BIOINFORMATIC ANALYSIS
.
.................
32
2.6 ANALYSIS OF REPORTERS CONTAINING
EP1
3 UTR...............................................32
2.6.1 CAT
ASSAY..........................................................................
33
2.6.2 NORTHERN
BLOTTING..............................................................
34
2.7 TRYPANOSOME
DIFFERENTIATION.................................................... 34
2.7.1 RBP10 MEDIATED TRYPANOSOME
DIFFERENTIATION.......................................... 35
2.7.2 ANALYSES OF CELL MORPHOLOGY AND DIFFERENTIATION MARKERS EXPRESSION
......
36
2.7.2.1 INDIRECT
IMMUNOFLUORESCENCE................................................................
.36
2.7.2 2 FLOW
CYTOMETRY.........................................................................................36
2.8 PROTEIN DETECTION
.................
37
2.9 LIST OF PLASMIDS AND OLIGONUCLEOTIDES
USED.................................................37
2.9.1 PLASMIDS
LIST.....................................................................................................
38
2.9.2 OLIGONUCLEOTIDES LIST
......................................
41
3 RESULTS
.......
.
..............................................
44
00 00 00
3.1 RBP10 C-TERMINUS PROMOTES REPORTER MRNA DESTRUCTION AND INHIBITS
TRANSLATION......................................................................................................................
44
3.2 TETHERED RBP10 SHIFTS REPORTER MRNA FROM THE POLYSOMES
...................
45
3.3 IDENTIFICATION OF RBP10 INTERACTING PROTEINS BY YEAST-TWO HYB RID
.........
47
3.3.1 HIGH THROUGHPUT YEAST TWO-HYBRID SCREEN USING RBP10 AS BAIT
...............
47
3.4 GENOME WIDE YEAST TWO-HYBRID DATA CONFIRMATION USING COMPLETE ORFS
52
3.5 RBP10, RBP26 AND 4E-IP INTERACTIONS IN BLOODSTREAM FORM
TRYPANOSOMES................................................................................................................53
3.6 RBP10 AND 4E-IP INTERDEPENDENCY IN PROCYCLIC FO RM S
.............................
55
3.6.1 INDEPENDENT DUAL INDUCTION SYSTEM USING TET AND IPTG IN PROCYCLIC
FORMS
55
3.7 TANDEM AFFINITY PURIFICATION OF
RBP10...........................................................59
3.8 RBP10 BIOLD IN BLOODSTREAM FORM
TRYPANOSOMES.......................................63
3.9 TRANSCRIPTOME WIDE EFFECTS OF RBP10 MANIPULATION IN
T. BRUCEI
...........
64
3.9.1 TIME POINT SELECTION FOR RNA
SEQUENCING.......................................................64
3.9.2 TRANSCRIPTOME CHANGES AFTER RBP10 DEPLETION FOR 15 HOURS
....................
68
3.9.3 TRANSCRIPTOME CHANGES AFTER RBP10 EXPRESSION FOR 6 HOURS IN
PROCYCLIC
CELLS 72
3.10 RBP10 MRNA
TARGETS.........................................................................................
75
3.11 RBP10 BINDING TO
EP3*
UTR
............................................................................
78
3.12 RBP10 AND TRYPANOSOME DIFFERENTIATION
.......................................................
80
3.12.1 BLOODSTREAM TO PROCYCLIC FORM CONVERSION
.....................................................
80
3.12.2 PROCYCLIC TO BLOODSTREAM FORM CONVERSION
....................................................
84
3.12.3TRANSCRIPTOME ANALYSIS OF DIFFERENTIATING PROCYCLIC CELLS
.............................
87
3.12.4METACYCLIC
VSGS
IDENTIFICATION
..........................................................................
93
4
DISCUSSION...................................................................................................................97
4.1 RBP10 IS A REPRESSOR
PROTEIN...........................................................................
97
4.2 RBP10 AFFECTS DEVELOPMENTAL REGULATION OF MANY MRNAS BOUND BY IT 98
4.3 ROLE OF RBP10 IN DIFFERENTIATION COMMITMENT
............................................
101
5
REFERENCES................................................................................................................
104
|
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| language | English |
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| spelling | Psychoanalysis, trauma and community history and contemporary reappraisals edited by Judith L. Alpert and Elizabeth R. Goren London and New York Routledge 2017 XX, 262 Seiten txt rdacontent n rdamedia nc rdacarrier Relational perspectives book series Includes bibliographical references and index Psychologie Psychotherapie Community psychology Psychic trauma Treatment Disasters Psychological aspects Psychotherapy Community mental health services Psychisches Trauma (DE-588)4060749-5 gnd rswk-swf Völkermord (DE-588)4063690-2 gnd rswk-swf Naturkatastrophe (DE-588)4041387-1 gnd rswk-swf Judenvernichtung (DE-588)4073091-8 gnd rswk-swf Kriegsopfer (DE-588)4165700-7 gnd rswk-swf Katastrophenreaktion (DE-588)4769377-0 gnd rswk-swf Kollektives Gedächtnis (DE-588)4200793-8 gnd rswk-swf Psychisches Trauma (DE-588)4060749-5 s Kriegsopfer (DE-588)4165700-7 s Naturkatastrophe (DE-588)4041387-1 s Judenvernichtung (DE-588)4073091-8 s Völkermord (DE-588)4063690-2 s Kollektives Gedächtnis (DE-588)4200793-8 s Katastrophenreaktion (DE-588)4769377-0 s DE-188 Alpert, Judith L. (DE-588)171948939 edt Goren, Elizabeth R. 1949- (DE-588)1122863934 edt Erscheint auch als Online-Ausgabe 9781315681948 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029359663&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Psychoanalysis, trauma and community history and contemporary reappraisals Psychologie Psychotherapie Community psychology Psychic trauma Treatment Disasters Psychological aspects Psychotherapy Community mental health services Psychisches Trauma (DE-588)4060749-5 gnd Völkermord (DE-588)4063690-2 gnd Naturkatastrophe (DE-588)4041387-1 gnd Judenvernichtung (DE-588)4073091-8 gnd Kriegsopfer (DE-588)4165700-7 gnd Katastrophenreaktion (DE-588)4769377-0 gnd Kollektives Gedächtnis (DE-588)4200793-8 gnd |
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| title | Psychoanalysis, trauma and community history and contemporary reappraisals |
| title_auth | Psychoanalysis, trauma and community history and contemporary reappraisals |
| title_exact_search | Psychoanalysis, trauma and community history and contemporary reappraisals |
| title_full | Psychoanalysis, trauma and community history and contemporary reappraisals edited by Judith L. Alpert and Elizabeth R. Goren |
| title_fullStr | Psychoanalysis, trauma and community history and contemporary reappraisals edited by Judith L. Alpert and Elizabeth R. Goren |
| title_full_unstemmed | Psychoanalysis, trauma and community history and contemporary reappraisals edited by Judith L. Alpert and Elizabeth R. Goren |
| title_short | Psychoanalysis, trauma and community |
| title_sort | psychoanalysis trauma and community history and contemporary reappraisals |
| title_sub | history and contemporary reappraisals |
| topic | Psychologie Psychotherapie Community psychology Psychic trauma Treatment Disasters Psychological aspects Psychotherapy Community mental health services Psychisches Trauma (DE-588)4060749-5 gnd Völkermord (DE-588)4063690-2 gnd Naturkatastrophe (DE-588)4041387-1 gnd Judenvernichtung (DE-588)4073091-8 gnd Kriegsopfer (DE-588)4165700-7 gnd Katastrophenreaktion (DE-588)4769377-0 gnd Kollektives Gedächtnis (DE-588)4200793-8 gnd |
| topic_facet | Psychologie Psychotherapie Community psychology Psychic trauma Treatment Disasters Psychological aspects Psychotherapy Community mental health services Psychisches Trauma Völkermord Naturkatastrophe Judenvernichtung Kriegsopfer Katastrophenreaktion Kollektives Gedächtnis |
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