Gene cloning and manipulation:
Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of clon...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Cambridge
Cambridge University Press
2007
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| Ausgabe: | Second edition |
| Schlagworte: | |
| Online-Zugang: | BSB01 FHN01 URL des Erstveröffentlichers |
| Zusammenfassung: | Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams |
| Beschreibung: | Title from publisher's bibliographic system (viewed on 05 Oct 2015) |
| Beschreibung: | 1 online resource (266 pages) |
| ISBN: | 9780511807343 |
| DOI: | 10.1017/CBO9780511807343 |
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| 505 | 8 | |a The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms | |
| 520 | |a Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams | ||
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Datensatz im Suchindex
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| author | Howe, C. J. |
| author_facet | Howe, C. J. |
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| author_sort | Howe, C. J. |
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| bvnumber | BV043942752 |
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| collection | ZDB-20-CBO |
| contents | The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms |
| ctrlnum | (ZDB-20-CBO)CR9780511807343 (OCoLC)967602496 (DE-599)BVBBV043942752 |
| dewey-full | 572.8/6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8/6 |
| dewey-search | 572.8/6 |
| dewey-sort | 3572.8 16 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1017/CBO9780511807343 |
| edition | Second edition |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| indexdate | 2024-07-10T07:39:18Z |
| institution | BVB |
| isbn | 9780511807343 |
| language | English |
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| spelling | Howe, C. J. Verfasser aut Gene cloning and manipulation Christopher Howe Gene Cloning & Manipulation Second edition Cambridge Cambridge University Press 2007 1 online resource (266 pages) txt rdacontent c rdamedia cr rdacarrier Title from publisher's bibliographic system (viewed on 05 Oct 2015) The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams Molecular cloning Genklonierung (DE-588)4123275-6 gnd rswk-swf Genklonierung (DE-588)4123275-6 s 1\p DE-604 Erscheint auch als Druckausgabe 978-0-521-52105-5 Erscheint auch als Druckausgabe 978-0-521-81793-6 https://doi.org/10.1017/CBO9780511807343 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Howe, C. J. Gene cloning and manipulation The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms Molecular cloning Genklonierung (DE-588)4123275-6 gnd |
| subject_GND | (DE-588)4123275-6 |
| title | Gene cloning and manipulation |
| title_alt | Gene Cloning & Manipulation |
| title_auth | Gene cloning and manipulation |
| title_exact_search | Gene cloning and manipulation |
| title_full | Gene cloning and manipulation Christopher Howe |
| title_fullStr | Gene cloning and manipulation Christopher Howe |
| title_full_unstemmed | Gene cloning and manipulation Christopher Howe |
| title_short | Gene cloning and manipulation |
| title_sort | gene cloning and manipulation |
| topic | Molecular cloning Genklonierung (DE-588)4123275-6 gnd |
| topic_facet | Molecular cloning Genklonierung |
| url | https://doi.org/10.1017/CBO9780511807343 |
| work_keys_str_mv | AT howecj genecloningandmanipulation AT howecj genecloningmanipulation |