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Ion channels: a laboratory manual

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Bibliographic Details
Other Authors:	Kammermeier, Paul J. (Editor), Duguid, Ian (Editor), Brenowitz, Stephan (Editor)
Format:	Book
Language:	English
Published:	New York Cold Spring Harbor Laboratory Press [2017]
Subjects:	Methode Ionenkanal Aufsatzsammlung
Online Access:	Inhaltsverzeichnis
Physical Description:	viii, 197 Seiten Illustrationen
ISBN:	9781621821205

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adam_text	Contents CHAPTER 1 Ion Channels: History, Diversity, and Impact INTRODUCTION Ion Channels: History, Diversity, and Impact 1 Stephan Brenowitz, Ian Duguidand Paul J. Kammermeier CHAPTER 2 Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating INTRODUCTION Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating 7 Dorothy M. Kim and Crina M. Nimigean CHAPTER 3 Voltage-Gated Na+ Channels: Not Just for Conduction INTRODUCTION Voltage-Gated Na+ Channels: Not Just for Conduction 23 Larisa C. Kruger and Lori L Isom CHAPTER 4 Measuring Ca2+-Dependent Modulation of Voltage-Gated Ca2+ Channels in HEK-293T Cells PROTOCOL 1 Measuring Ca2+-Dependent Modulation of Voltage-Gated Ca2f Channels in HEK-293T Cells 39 Jessica R. Thomas and Amy Lee CHAPTER 5 Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca2+ Channels INTRODUCTION Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca2+ Channels 45 Van B. Lu and Stephen R. Ikeda PROTOCOL 1 G-Protein Modulation of Voltage-Gated Ca2+ Channels from Isolated Adult Rat Superior Cervical Ganglion Neurons 54 Van B. Lu and Stephen R. Ikeda V vi / Contents CHAPTER 6 Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents in Neurons INTRODUCTION Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents in Neurons 63 Mala M. Shah PROTOCOL 1 Recording Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents (/h) in Neurons 66 Mala M. Shah CHAPTER 7 Single-Channel Recording of Ligand-Gated Ion Channels INTRODUCTION Single-Channel Recording of Ligand-Gated Ion Channels 73 Andrew J.R. Plested PROTOCOL 1 Single-Channel Recording of Glycine Receptors in Human Embryonic Kidney (HEK) Cells 79 Andrew J.R. Plested and Jelena Baranovlc CHAPTER 8 Measuring the Basic Physiological Properties of Synapses INTRODUCTION Measuring the Basic Physiological Properties of Synapses 85 Matthew A. Xu-Friedman PROTOCOL 1 Preparing Brain Slices to Study Basic Synaptic Properties 89 Matthew A. Xu-Friedman CHAPTER 9 Cellular and Synaptic Properties of Local Inhibitory Circuits INTRODUCTION Cellular and Synaptic Properties of Local Inhibitory Circuits 97 Court Hull PROTOCOL 1 Measuring Feedforward Inhibition and Its impact on Local Circuit Function Court Hull 101 Contents / vii CHAPTER 10 Fast Cholinergic Synaptic Transmission in the Mammalian Central Nervous System INTRODUCTION Fast Cholinergic Synaptic Transmission in the Mammalian Central Nervous System 107 Michael Beierlein PROTOCOL 1 Examining Cholinergic Synaptic Signaling in the Thalamic Reticular Nucleus 111 Rajan Dasgupta, Frederik Seibt, Yan-Gang Sun, and Michael Beierlein CHAPTER 11 In Vitro Investigation of Synaptic Plasticity INTRODUCTION In Vitro Investigation of Synaptic Plasticity 117 Therese Abrahamsson, Txomin Lalanne, Alanna /. Watt, and P. lesper Sjostrom PROTOCOLS 1 Long-Term Potentiation by Theta-Burst Stimulation Using Extracellular Field Potential Recordings in Acute Hippocampal Slices 120 Therese Abrahamsson, Txomin Lalanne, Alanna J. Watt; and P. lesper Sjostrom 2 Using Multiple Whole-Cell Recordings to Study Spike-Timing-Dependent Plasticity in Acute Neocortical Slices 129 Txomin Lalanne, Therese Abrahamsson, and P. lesper Sjostrom CHAPTER 12 Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels INTRODUCTION Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels 141 /. Simon Wiegert, Christine E. Cee, and Thomas G. Oertner PROTOCOLS 1 Preparation of Slice Cultures from Rodent Hippocampus 148 Christine E. Gee, Iris Ohmert, f. Simon Wiegert, and Thomas G. Oertner 2 Viral Vector-Based Transduction of Slice Cultures 153 J. Simon Wiegert, Christine E. Gee, and Thomas G. Oertner 3 Single-Cell Electroporation of Neurons 157 /. Simon Wiegert, Christine E. Gee, and Thomas G. Oertner viii / Contents CHAPTER 13 Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits PROTOCOL 1 Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits 161 Naoki Yamawaki, Benjamin A. Suter, Ian R. Wickersham, and Cordon M.G. Shepherd CHAPTER 14 Whole-Cell Recording in the Awake Brain INTRODUCTION Whole-Cell Recording in the Awake Brain 169 Doyun Lee and Albert K. Lee PROTOCOLS 1 In Vivo Patch-Clamp Recording in Awake Head-Fixed Rodents 173 Doyun Lee and Albert K. Lee 2 Efficient Method for Whole-Cel! Recording in Freely Moving Rodents Using Ultraviolet-Cured Collar-Based Pipette Stabilization 180 Doyun Lee and Albert K. Lee APPENDIX General Safety and Hazardous Material Information 189 INDEX 195
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spelling	Ion channels a laboratory manual edited by Paul J. Kammermeier (University of Rochester Medical Center), Ian Duguid (Centre for Integrative Physiology, University of Edinburgh), Stephan Brenowitz (Janelia Research Campus, Howard Hughes Medical Institute) New York Cold Spring Harbor Laboratory Press [2017] viii, 197 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methode (DE-588)4038971-6 gnd rswk-swf Ionenkanal (DE-588)4138699-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Ionenkanal (DE-588)4138699-1 s Methode (DE-588)4038971-6 s DE-604 Kammermeier, Paul J. (DE-588)1134899785 edt Duguid, Ian (DE-588)1134900104 edt Brenowitz, Stephan (DE-588)1134900341 edt Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029343629&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
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title	Ion channels a laboratory manual
title_auth	Ion channels a laboratory manual
title_exact_search	Ion channels a laboratory manual
title_full	Ion channels a laboratory manual edited by Paul J. Kammermeier (University of Rochester Medical Center), Ian Duguid (Centre for Integrative Physiology, University of Edinburgh), Stephan Brenowitz (Janelia Research Campus, Howard Hughes Medical Institute)
title_fullStr	Ion channels a laboratory manual edited by Paul J. Kammermeier (University of Rochester Medical Center), Ian Duguid (Centre for Integrative Physiology, University of Edinburgh), Stephan Brenowitz (Janelia Research Campus, Howard Hughes Medical Institute)
title_full_unstemmed	Ion channels a laboratory manual edited by Paul J. Kammermeier (University of Rochester Medical Center), Ian Duguid (Centre for Integrative Physiology, University of Edinburgh), Stephan Brenowitz (Janelia Research Campus, Howard Hughes Medical Institute)
title_short	Ion channels
title_sort	ion channels a laboratory manual
title_sub	a laboratory manual
topic	Methode (DE-588)4038971-6 gnd Ionenkanal (DE-588)4138699-1 gnd
topic_facet	Methode Ionenkanal Aufsatzsammlung
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