Ion channels: a laboratory manual
Gespeichert in:
| Weitere Verfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Cold Spring Harbor Laboratory Press
[2017]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | viii, 197 Seiten Illustrationen |
| ISBN: | 9781621821205 |
Internformat
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Datensatz im Suchindex
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| adam_text | Contents
CHAPTER 1 Ion Channels: History, Diversity, and Impact
INTRODUCTION
Ion Channels: History, Diversity, and Impact 1
Stephan Brenowitz, Ian Duguidand Paul J. Kammermeier
CHAPTER 2 Voltage-Gated Potassium Channels: A Structural Examination of Selectivity
and Gating
INTRODUCTION
Voltage-Gated Potassium Channels: A Structural Examination of Selectivity
and Gating 7
Dorothy M. Kim and Crina M. Nimigean
CHAPTER 3 Voltage-Gated Na+ Channels: Not Just for Conduction
INTRODUCTION
Voltage-Gated Na+ Channels: Not Just for Conduction 23
Larisa C. Kruger and Lori L Isom
CHAPTER 4 Measuring Ca2+-Dependent Modulation of Voltage-Gated Ca2+ Channels
in HEK-293T Cells
PROTOCOL
1 Measuring Ca2+-Dependent Modulation of Voltage-Gated Ca2f Channels
in HEK-293T Cells 39
Jessica R. Thomas and Amy Lee
CHAPTER 5 Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca2+ Channels
INTRODUCTION
Strategies for Investigating G-Protein Modulation of Voltage-Gated Ca2+ Channels 45
Van B. Lu and Stephen R. Ikeda
PROTOCOL
1 G-Protein Modulation of Voltage-Gated Ca2+ Channels from Isolated
Adult Rat Superior Cervical Ganglion Neurons 54
Van B. Lu and Stephen R. Ikeda
V
vi / Contents
CHAPTER 6 Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents in Neurons
INTRODUCTION
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel
Currents in Neurons 63
Mala M. Shah
PROTOCOL
1 Recording Hyperpolarization-Activated Cyclic Nucleotide-Gated
Channel Currents (/h) in Neurons 66
Mala M. Shah
CHAPTER 7 Single-Channel Recording of Ligand-Gated Ion Channels
INTRODUCTION
Single-Channel Recording of Ligand-Gated Ion Channels 73
Andrew J.R. Plested
PROTOCOL
1 Single-Channel Recording of Glycine Receptors in Human Embryonic
Kidney (HEK) Cells 79
Andrew J.R. Plested and Jelena Baranovlc
CHAPTER 8 Measuring the Basic Physiological Properties of Synapses
INTRODUCTION
Measuring the Basic Physiological Properties of Synapses 85
Matthew A. Xu-Friedman
PROTOCOL
1 Preparing Brain Slices to Study Basic Synaptic Properties 89
Matthew A. Xu-Friedman
CHAPTER 9 Cellular and Synaptic Properties of Local Inhibitory Circuits
INTRODUCTION
Cellular and Synaptic Properties of Local Inhibitory Circuits 97
Court Hull
PROTOCOL
1 Measuring Feedforward Inhibition and Its impact on Local Circuit Function
Court Hull
101
Contents / vii
CHAPTER 10 Fast Cholinergic Synaptic Transmission in the Mammalian Central Nervous System
INTRODUCTION
Fast Cholinergic Synaptic Transmission in the Mammalian Central Nervous System 107
Michael Beierlein
PROTOCOL
1 Examining Cholinergic Synaptic Signaling in the Thalamic
Reticular Nucleus 111
Rajan Dasgupta, Frederik Seibt, Yan-Gang Sun, and Michael Beierlein
CHAPTER 11 In Vitro Investigation of Synaptic Plasticity
INTRODUCTION
In Vitro Investigation of Synaptic Plasticity 117
Therese Abrahamsson, Txomin Lalanne, Alanna /. Watt, and P. lesper Sjostrom
PROTOCOLS
1 Long-Term Potentiation by Theta-Burst Stimulation Using Extracellular
Field Potential Recordings in Acute Hippocampal Slices 120
Therese Abrahamsson, Txomin Lalanne, Alanna J. Watt; and P. lesper Sjostrom
2 Using Multiple Whole-Cell Recordings to Study Spike-Timing-Dependent
Plasticity in Acute Neocortical Slices 129
Txomin Lalanne, Therese Abrahamsson, and P. lesper Sjostrom
CHAPTER 12 Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels
INTRODUCTION
Stimulating Neurons with Heterologously Expressed Light-Gated Ion Channels 141
/. Simon Wiegert, Christine E. Cee, and Thomas G. Oertner
PROTOCOLS
1 Preparation of Slice Cultures from Rodent Hippocampus 148
Christine E. Gee, Iris Ohmert, f. Simon Wiegert, and Thomas G. Oertner
2 Viral Vector-Based Transduction of Slice Cultures 153
J. Simon Wiegert, Christine E. Gee, and Thomas G. Oertner
3 Single-Cell Electroporation of Neurons 157
/. Simon Wiegert, Christine E. Gee, and Thomas G. Oertner
viii / Contents
CHAPTER 13 Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits
PROTOCOL
1 Combining Optogenetics and Electrophysiology to Analyze Projection
Neuron Circuits 161
Naoki Yamawaki, Benjamin A. Suter, Ian R. Wickersham, and Cordon M.G. Shepherd
CHAPTER 14 Whole-Cell Recording in the Awake Brain
INTRODUCTION
Whole-Cell Recording in the Awake Brain 169
Doyun Lee and Albert K. Lee
PROTOCOLS
1 In Vivo Patch-Clamp Recording in Awake Head-Fixed Rodents 173
Doyun Lee and Albert K. Lee
2 Efficient Method for Whole-Cel! Recording in Freely Moving Rodents
Using Ultraviolet-Cured Collar-Based Pipette Stabilization 180
Doyun Lee and Albert K. Lee
APPENDIX General Safety and Hazardous Material Information 189
INDEX 195
|
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| spelling | Ion channels a laboratory manual edited by Paul J. Kammermeier (University of Rochester Medical Center), Ian Duguid (Centre for Integrative Physiology, University of Edinburgh), Stephan Brenowitz (Janelia Research Campus, Howard Hughes Medical Institute) New York Cold Spring Harbor Laboratory Press [2017] viii, 197 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methode (DE-588)4038971-6 gnd rswk-swf Ionenkanal (DE-588)4138699-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Ionenkanal (DE-588)4138699-1 s Methode (DE-588)4038971-6 s DE-604 Kammermeier, Paul J. (DE-588)1134899785 edt Duguid, Ian (DE-588)1134900104 edt Brenowitz, Stephan (DE-588)1134900341 edt Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029343629&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Ion channels a laboratory manual |
| title_auth | Ion channels a laboratory manual |
| title_exact_search | Ion channels a laboratory manual |
| title_full | Ion channels a laboratory manual edited by Paul J. Kammermeier (University of Rochester Medical Center), Ian Duguid (Centre for Integrative Physiology, University of Edinburgh), Stephan Brenowitz (Janelia Research Campus, Howard Hughes Medical Institute) |
| title_fullStr | Ion channels a laboratory manual edited by Paul J. Kammermeier (University of Rochester Medical Center), Ian Duguid (Centre for Integrative Physiology, University of Edinburgh), Stephan Brenowitz (Janelia Research Campus, Howard Hughes Medical Institute) |
| title_full_unstemmed | Ion channels a laboratory manual edited by Paul J. Kammermeier (University of Rochester Medical Center), Ian Duguid (Centre for Integrative Physiology, University of Edinburgh), Stephan Brenowitz (Janelia Research Campus, Howard Hughes Medical Institute) |
| title_short | Ion channels |
| title_sort | ion channels a laboratory manual |
| title_sub | a laboratory manual |
| topic | Methode (DE-588)4038971-6 gnd Ionenkanal (DE-588)4138699-1 gnd |
| topic_facet | Methode Ionenkanal Aufsatzsammlung |
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