Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.):
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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Köln
2016
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| Beschreibung: | IX, 116 Seiten Diagramme 21 cm |
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| 245 | 1 | 0 | |a Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) |c vorgelegt von Mohamed Aman Mulki |
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Datensatz im Suchindex
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| adam_text | Titel: Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.)
Autor: Mulki, Mohamed Aman
Jahr: 2016
Table of Contents
Chapter One: Barley CONSTANS Orthologs HvC01/HvC02 Mediate the Photoperiodic
Regulation of the Floral Repressor VRN-H2 1
Abstract 2
Introduction 4
Results 10
Overexpression of HvC02 accelerated flowering time in a spring barley background . 10
Overexpression of HvC02 did not overcome the vernalization requirement............11
Overexpression of HvC02 upregulated the floral repressor VRN-H2....................13
Overexpression of HvC02 and HvCOl induced expression of VRN-H2 and HvFTl
15
under SD condition......................................
Discussion 17
Overexpression of HvC02 causes photo period-dependent early flowering in barley . 17
Overexpression of HvC02 does not overcome vernalization requirement ............18
Conclusion....................................................................................20
Materials and Methods 22
Generation and growth conditions of the transgenic Ubi::HvC02 lines..................22
Generation and growth conditions of Ubi::HvC02 X Igri and Ubi::HvC01 X Igri Fz
populations......................................................................................23
DNA extraction and genotyping of the segregating populations ............ 24
RNA extraction, cDNA synthesis and quantitative real time PCR (qRT-PCR)..............24
Statistical analysis..............................................................................24
References 26
Tables 32
Figures 38
Chapter Two: HvFT3 Promotes Floral Transition and Early Reproductive Growth but is not
Sufficient for Successful Flowering under Non-inductive Conditions 52
Abstract 53
Introduction 55
Results 61
Overexpression of HvFT3 accelerated flowering time of spring barley under LD but
not under SD conditions......................................................................61
Overexpression of HvFT3 accelerated phase transition and early reproductive
inflorescence development under SD and LD..............................................61
Diurnal expression of major flowering genes in Ubi::HvFT3 under LD and SD
condition......................................................................................63
Overexpression of HvFT3 induced expression of API-like genes in leaf and at SAM
under LD condition......................................
Overexpression of HvFT3 accelerated flowering time in winter barley ................65
Overexpression of HvFT3 downregulated VRN-FI2 expression in winter barley .... 67
The effect of functional variation of HvFT3 on plant development under SD and LD
and its interaction with Ppd-Hl..............................................................68
HvFT3 Accelerated flowering Time of non-vernalized or SD-treated winter lines ... 70
Discussion 72
HvFT3 is not sufficient for flowering under non-inductive SD conditions................72
HvFT3 accelerates phase transition under SD and LD......................................72
VRN-H1 mediates FlvFT3 acceleration of floral transition under SD and LD conditions 73
Overexpression of HvFT3 overcomes vernalization requirement........................74
Short day vernalization SD induction is mediated by FlvFT3 in winter barley
genotypes......................................................................................76
Ppd-Hl allele diversity modulates HvFT3 effect on plant development ................77
Conclusion......................................................................................77
Materials and Methods 79
Generation and growth conditions of the transgenic Ubi::HvFT3 lines..................79
Inflorescence development of transgenic Ubi::HvFT3 lines ..............................80
Diurnal gene expression in the leaves of Ubi::HvFT3 plants (line N16)..................80
Gene expression in the leaves and at the SAM of Ubi::HvFT3 line (N23)................80
Generation and growth conditions of Ubi::HvFT3 x Igri F2 population....................81
Generation, growth conditions and inflorescence development of HvFT3 F„ spring
families..........................................................................................81
Generation, growth conditions and the vernalization/short-day response experiment
of HvFT3 F4 winter families..................................................................82
DNA extraction and genotyping of the segregating populations..........................83
RNA extraction, cDNA synthesis and quantitative real time PCR (qRT-PCR) ............83
Statistical analysis............................................................................83
References 84
Tables 89
Figures 92
Supplementary Table 1 111
Acknowledgements 113
Erklärung 114
Lebenslauf 115
|
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| language | English |
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| spelling | Mulki, Mohamed Aman 1981- Verfasser (DE-588)1104762099 aut Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) vorgelegt von Mohamed Aman Mulki Köln 2016 IX, 116 Seiten Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität zu Köln 2016 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1110918119/04 Inhaltsverzeichnis HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029268994&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mulki, Mohamed Aman 1981- Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) |
| subject_GND | (DE-588)4113937-9 |
| title | Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) |
| title_auth | Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) |
| title_exact_search | Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) |
| title_full | Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) vorgelegt von Mohamed Aman Mulki |
| title_fullStr | Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) vorgelegt von Mohamed Aman Mulki |
| title_full_unstemmed | Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) vorgelegt von Mohamed Aman Mulki |
| title_short | Functional characterization of photoperiod response genes in Barley (Hordeum vulgare L.) |
| title_sort | functional characterization of photoperiod response genes in barley hordeum vulgare l |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1110918119/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029268994&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mulkimohamedaman functionalcharacterizationofphotoperiodresponsegenesinbarleyhordeumvulgarel |
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