Functional analysis of Strubbelig Receptor Family 3: a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana
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| Sprache: | English |
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2015
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| Beschreibung: | 2 Mikrofiches (XIV, 151 Seiten) Illustrationen, Diagramme |
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| 245 | 1 | 0 | |a Functional analysis of Strubbelig Receptor Family 3 |b a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana |c vorgelegt von Marcel von Reth |
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| _version_ | 1804176674755969025 |
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| adam_text | Titel: Functional analysis of Strubbelig Receptor Family 3
Autor: Reth, Marcel von
Jahr: 2015
Table of Contents
Publications I
Abstract III
Zusammenfassung V
Abbreviations XI
1. Introduction 1
1.1. Hybrid necrosis is frequently caused by a mismatch between plant immune
system components 1
1.2. The plant immune system and factors in immune-related HI 4
1.3. Immune-related HI between Ler and Central Asian accessions 14
1.3.1. Plant LRR-RLKs and the STRUBBELIG RECEPTOR FAMILY 21
1.4. Aims of this work 24
2. Results 27
2.1. Introgression of T-DNA insertion alleles into an autoimmune genetic
background 27
2.2. Generation and phenotypic characterization of stable transgenic Arabidopsis
expressing SRF3^er- and SRF3Kas 2 C-terminal fusion proteins 32
2.3. Sub-cellular localization of SRF3-GFP 40
2.3.1. SRF3-GFP localization in rosette leaves of stable transgenic lines 41
2.3.2. Co-localization of Aniline Blue-stained callose and SRF3-GFP signals at
plasmodesmata 45
2.3.3. SRF3-GFP accumulation at plasmodesmata in various plant tissues and
organs 47
2.3.4. Transmission electron microscopy (TEM) and immuno-gold labeling (IGL)
confirm plasmodesmal localization of SRF3-GFP 49
2.4. Identification of SRF3-associated proteins 54
2.4.1. SRF3Ler-GFP associates with callose synthases and core PTI signalling
components 54
2.4.2. Analysis of the flg22-triggered transcriptional response of SAP-encoding
genes 61
2.5. Assigning a basic function to SRF3 in PTI 63
2.5.1. SRF3 modifies molecular flux in isogenic backgrounds 66
2.5.2. Flg22-triggered ROS burst is enhanced in cKas-2 69
2.5.3. Flg22-triggered seedling growth inhibition is suppressed in cKas-2 72
2.5.4. Summary: SRF3Ler differentially affects PTI signalling outputs in Kas-2
background 74
3. Discussion 76
3.1. Expression differences of SRF3Col~0 do not explain immune-related HI in Ler x
Kas-2 76
3.2. Epitope-tagged SRF3Kas 2 unexpectedly suppresses autoimmunity-related
growth retardation 77
3.3. SRF3-GFP accumulates at plasmodesmata 81
3.4. SRF3Ler-GFP associates with proteins involved in plant immunity and callose
synthesis 83
3.4.1. PRRs and other RLKs involved in plant immunity 85
3.4.2. Respiratory burst oxidase homologue D (RBOHD) 86
3.4.3. Callose synthases 87
3.5. SRF3t-m differentially affects PTI signalling outputs in Kas-2 genetic background
89
3.5.1. SRF3Le enhances flg22-triggered restriction in PD-flux 90
3.5.2. ROS is enhanced in cKas-2 91
3.5.3. Flg22-mediated seedling growth inhibition is suppressed in cKas-2 92
3.6. Conclusions and future directions 92
4. Materials and Methods 97
4.1. Materials 97
4.1.1. Plant materials 97
4.1.2. Bacterial strains 98
4.1.3. Vectors 99
4.1.4. Antibodies 101
4.1.5. Oligonucleotides 102
4.1.6. Enzymes 104
4.1.7. Chemicals 105
4.1.8. Antibiotics (stock solutions) 105
4.1.9. Media 106
4.1.10. Buffers and solutions 107
4.2. Methods 109
4.2.1. Plant methods 109
4.2.2. Microscopy 114
4.2.3. Molecular biology methods 116
4.2.4. Biochemical methods 123
4.2.5. Large-scale discovery immunoprecipitation from Arabidopsis thaliana-
whole plant tissue 125
5. Literature cited 129
6. Supplementary information 143
Acknowledgements 147
Erklarung 149
Lebenslauf 151
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| spelling | von Reth, Marcel 1984- Verfasser (DE-588)1104724103 aut Functional analysis of Strubbelig Receptor Family 3 a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana vorgelegt von Marcel von Reth Köln 2015 2 Mikrofiches (XIV, 151 Seiten) Illustrationen, Diagramme txt rdacontent h rdamedia he rdacarrier 24x Dissertation Universität zu Köln 2015 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1113672730/04 Inhaltsverzeichnis HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029230792&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | von Reth, Marcel 1984- Functional analysis of Strubbelig Receptor Family 3 a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana |
| subject_GND | (DE-588)4113937-9 |
| title | Functional analysis of Strubbelig Receptor Family 3 a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana |
| title_auth | Functional analysis of Strubbelig Receptor Family 3 a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana |
| title_exact_search | Functional analysis of Strubbelig Receptor Family 3 a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana |
| title_full | Functional analysis of Strubbelig Receptor Family 3 a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana vorgelegt von Marcel von Reth |
| title_fullStr | Functional analysis of Strubbelig Receptor Family 3 a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana vorgelegt von Marcel von Reth |
| title_full_unstemmed | Functional analysis of Strubbelig Receptor Family 3 a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana vorgelegt von Marcel von Reth |
| title_short | Functional analysis of Strubbelig Receptor Family 3 |
| title_sort | functional analysis of strubbelig receptor family 3 a receptor like kinase involved in hybrid incompatibility in arabidopsis thaliana |
| title_sub | a receptor-like kinase involved in hybrid incompatibility in Arabidopsis thaliana |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1113672730/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029230792&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT vonrethmarcel functionalanalysisofstrubbeligreceptorfamily3areceptorlikekinaseinvolvedinhybridincompatibilityinarabidopsisthaliana |
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