Genetic analysis of trichome and root hair development in Arabis alpina:
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| Format: | Abschlussarbeit Mikrofilm Buch |
| Sprache: | English |
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Köln
2015
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| Beschreibung: | 2 Mikrofiches (XVIII, 111 Seiten, Seiten a-c) Illustrationen, Diagramme |
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| 245 | 1 | 0 | |a Genetic analysis of trichome and root hair development in Arabis alpina |c vorgelegt von Divykriti Chopra |
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Datensatz im Suchindex
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| adam_text | Titel: Genetic analysis of trichome and root hair development in Arabis alpina
Autor: Chopra, Divykriti
Jahr: 2015
Contents
...........................V
Acknowledgments.....................................................................................
..............viii
Zusammenfassung...................................................................................
.................x
Abstract.....................................................................................................
................xi
Publication.................................................................................................
...............xii
List of Figures...........................................................................................
List of Tables...................................................................................................................xiv
List of Abbreviations.......................................................................................................xv
1. Introduction..........................1
1.1. The TTG1 gene regulatory network....................................................................1
1.2. Proanthocyanidin and anthocyanidin production............................................4
1.3. Seed coat mucilage production..........................................................................4
1.4. Trichome development........................................................................................5
1.5. Root hair development........................................................................................7
1.6. Variability and constraints of the TTG1 gene network.....................................9
1.7. Evo-Devo helps to understand phenotypic changes.....................................19
1.8. Evolution of the TTG1 gene network................................................................
1.9. TTG1 gene network in Brassicaceae................................................................12
1.10. A. alpina: A satellite model to study the TTG1 gene network.....................13
2. Aim of this work........................................................................................................14
3. Results.......................................................................................................................15
3.1. Trichome morphogenesis in A. alpina.............................................................15
3.2. Trichome patterning in A. alpina......................................................................17
3.3. Root hair patterning in A. alpina.......................................................................20
3.4. Trichome mutants obtained from EMS screen of A. alpina...........................22
3.5. In silico search of genes in A. alpina...............................................................26
3.6. Sequencing of identified mutants....................................................................30
3.7. Analysis of Aa TTG1 function............................................................................32
3.8. Analysis of AaGL3 function..........................................................................
3.9. Curious case of the glabrous mutants........................................................................45
3.10. Analysis of AaTRYfunction..............................................................................................48
3.11. Analysis of AaGL2 function................................................................................51
3.12. Phenotypic analysis of patchy mutants in A. alpina....................................56
3.13. Analysis of distorted mutants in A. alpina....................................................58
3.14. Analysis of glassy trichoma mutants in A. alpina........................................61
3.15. Analysis of unbranched mutants in A. alpina...............................................62
3.16. Analysis of irregularly branched mutants in A. alpina.................................63
3.17. Protein interaction studies of TTG1 gene regulatory network....................65
3.18. Root hair mutants obtained from EMS screen of A. alpina.........................65
4. Discussion.................................................................................................................68
4.1. Trichome mutants isolated from EMS screens of A. alpina...........................68
4.2. Five traits controlled by the TTG1 gene network in A. alpina........................69
4.3. Trichome pattern in A. alpina differs from A. thaliana...................................70
4.4. Root hair pattern in A. alpina differs from A. thaliana....................................71
4.5. Sequence divergence of TTG1 gene network in A. alpina.............................72
4.6. MBW complex in A. thaliana vs. A. alpina.......................................................73
4.7. Unexplained glabrous mutants in A. alpina....................................................76
4.8. Novel Patchy mutants in A. alpina...................................................................77
4.9. Morphology mutants in A. alpina......................................................................77
4.10. Root Hair mutants isolated from EMS screen of A. alpina..........................79
4.11. Conclusion.......................................................................................................80
5. Materials and methods.............................................................................................81
5.1. Plant Material and growth conditions..............................................................81
5.2. Synteny and sequence analysis.......................................................................81
5.3. Trichome analysis..............................................................................................82
5.4. Root hair file analysis........................................................................................82
5.5. Nucleic acid extraction......................................................................................82
5.6. PGR......................................................................................................................84
5.7. DNA Sequencing................................................................................................84
5.8. Transformation...................................................................................................85
5.9. Constructs and stable plant transformation...................................................86
5.10. Yeast two hybrid assays.................................................................................87
5.11. Photography and microscopy........................................................................88
5.12. Statistical analysis and software....................................................................89
6. References................................................................................................................80
7. Supplementary Material.........................................................................................105
Erklarung............................................................................................................................a
Lebenslauf.........................................................................................................................b
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| discipline | Biologie |
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| language | English |
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| physical | 2 Mikrofiches (XVIII, 111 Seiten, Seiten a-c) Illustrationen, Diagramme |
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| spelling | Chopra, Divykriti Verfasser aut Genetic analysis of trichome and root hair development in Arabis alpina vorgelegt von Divykriti Chopra Köln 2015 2 Mikrofiches (XVIII, 111 Seiten, Seiten a-c) Illustrationen, Diagramme txt rdacontent h rdamedia he rdacarrier 24x Dissertation Universität zu Köln 2015 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf http://d-nb.info/1110975813/04 Inhaltsverzeichnis HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029230695&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chopra, Divykriti Genetic analysis of trichome and root hair development in Arabis alpina |
| subject_GND | (DE-588)4113937-9 |
| title | Genetic analysis of trichome and root hair development in Arabis alpina |
| title_auth | Genetic analysis of trichome and root hair development in Arabis alpina |
| title_exact_search | Genetic analysis of trichome and root hair development in Arabis alpina |
| title_full | Genetic analysis of trichome and root hair development in Arabis alpina vorgelegt von Divykriti Chopra |
| title_fullStr | Genetic analysis of trichome and root hair development in Arabis alpina vorgelegt von Divykriti Chopra |
| title_full_unstemmed | Genetic analysis of trichome and root hair development in Arabis alpina vorgelegt von Divykriti Chopra |
| title_short | Genetic analysis of trichome and root hair development in Arabis alpina |
| title_sort | genetic analysis of trichome and root hair development in arabis alpina |
| topic_facet | Hochschulschrift |
| url | http://d-nb.info/1110975813/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029230695&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT chopradivykriti geneticanalysisoftrichomeandroothairdevelopmentinarabisalpina |
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