Septins:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam
Elsevier
2016
|
| Ausgabe: | First edition 2016 |
| Schriftenreihe: | Methods in cell biology
volume 136 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 365 Seiten, 19 ungezählte Seiten Illustrationen |
| ISBN: | 9780128039984 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804176492828033024 |
|---|---|
| adam_text | Contents
Contributors.......................................................... xi
Preface.................................................................xv
CHAPTER 1 The Nonopisthokont Septins: How Many There Are,
How Little We Know About Them, and How We
Might Learn More.........................................................1
M. Onishi, J.R. Pringle
Introduction.............................................2
1. The Wide Phylogenetic Distribution and Inferred Ancient
Origin of the Septins....................................3
2. The Nearly Total Lack of Functional Information About the
Nonopisthokont Septins................................ 14
3. The Prospects for Insights From Functional Analyses of
Nonopisthokont Septins..................................15
Acknowledgments.........................................16
References..............................................17
CHAPTER 2 Preparing Recombinant Yeast Septins and Their
Analysis by Electron Microscopy.........................21
A. Bertin, E. Nogales
Introduction............................................22
1. Methods............................*...................23
2. Materials........................................... 26
3. Materials..............................................27
4- Materials..............................................30
Conclusion..............................................32
Acknowledgments....................................... 33
References............................................ 33
CHAPTER 3 A FRET-Based Method for Monitoring Septin
Polymerization and Binding of Septin-Associated
Proteins............................................... 35
E.A. Booth, i. Thorner
Introduction.......................................... 36
1. Protein Preparation and Labeling........................41
2. Data Collection and Analysis...........................47
vi
Contents
Conclusions and Outlook..................................51
Acknowledgments..........................................51
References...............................................51
CHAPTER 4 In Vitro Reconstitution of Septin Assemblies on
Supported Lipid Bilayers.................................57
A.A. Bridges, A.S. Gladfelter
Introduction.............................................58
1. Rational.................................................58
2. Cleaning of Glass Coverslips.............................62
3. Preparation of Small Unilamellar Vesicles................64
4. Formation of Supported Lipid Bilayers....................67
5. Visualizing Septin Adsorption and Polymerization on
Supported Lipid Bilayers.................................68
Concluding Remarks.......*...............................69
References...............................................69
CHAPTER 5 Visualization of In Vivo Septin Ultrastructures
by Platinum Replica Electron Microscopy..................73
K. Ong, T. Svitkina, E. Bi
Introduction.............................................74
1. Preparation of Cell Cortices by Unroofing Spheroplasts...78
2. Platinum Replica Production..............................83
3. Imaging and Analysis.....................................91
4. Correlative Light and Electron Microscopy................92
Acknowledgments........................................ 95
References...............................................95
CHAPTER 6 Assays for Genetic Dissection of Septin Filament
Assembly in Yeasty From De Novo Folding
Through Polymerization................................. 99
NLA. NIcMurray
1. Background........................................ 100
2. The Logic of Obtaining and Interpreting Simple
Phenotypes............................................. 102
3. Summary and Perspective............................... 112
References............................................ 112
Contents
CHAPTER 7 Investigation of Septins Using Infection by
Bacterial Pathogens...................................117
S. Krokowski, S. Mostowy
Introduction..........................................118
1. Methods...............................................120
Conclusion............................................131
Acknowledgments.......................................131
References.......................................... 131
CHAPTER 8 In Vivo Analysis of Septin Heteropolymer
Rods and Higher-Order Structures in
Filamentous Fungi.....................................135
A. Smith, M. Momany
1. Protocol: Expression, Observation, and Purification of
Septin HOS and Heteropolymer Rods.....................137
References............................................140
CHAPTER 9 Live Cell Imaging of Septin Dynamics in Ustilago
Maydis............................................ 143
S. Baumann, S. Zander, S. Weidtkamp-Peters,
M. Feldbriigge
Introduction..........................................144
1. Methods...............................................147
Conclusions...........................................157
Acknowledgments.......................................157
Supplementary data....................................157
References.......................................... 157
CHAPTER 10 Ashbya gossypii as a Model System to Study
Septin Organization by Single-Molecule
Localization Microscopy...............................161
C. Kaplan, C. Yu, H. Ewers
Introduction....................................... 162
1. Considerations of Ashbya gossypii Sample Preparation
Strategy for Single-Molecule Localization Microscopy..167
2. Preparation of Septin Structures in Ashbya gossypii for
Single-Molecule Localization Microscopy...............170
3. Single-Molecule Localization Image Acquisitions......174
4. Example Images.......................................177
viii Contents
Summary................................................177
Acknowledgments...................................... 178
References............................................178
CHAPTER 11 Visualizing Septins in Early Drosophila
embryos.......................................... 183
M. Mavrakis
Introduction...........................................184
1, Preparation of Early Drosophila Embryos for
Immunofluorescence Detection of Septins................185
2. Preparation of Early Drosophila Embryos for Live
Fluorescence Imaging of Fluorescent Protein Fusions of
Septins................................................194
References........................................... 197
CHAPTER 12 Purification of Recombinant Human and
Drosophila Septin Hexamers for T1RF Assays of
Actin-Septin Filament Assembly......................199
M. Mavrakis, F.-C. Tsai, G.H. Koenderink
Introduction...........................................200
1. Cloning Strategy for Recombinant Septin Complex
Production in Bacteria.................................202
2. Expression of Recombinant Septin Complexes in Bacteria.... 203
3. Purification and Characterization of Recombinant Septin
Complexes From Bacteria................................205
4. Labeling Septins for Total Internal Reflection Fluorescence
Imaging...............................................209
5. Building Flow Cells for Total Internal Reflection
Fluorescence Microscopy........................... 211
6. In Vitro Reconstitution of Actin—Septin Filament
Assembly..................... ........................214
7. Total Internal Reflection Fluorescence Microscopy......217
Acknowledgments.......................................218
References..................................... 218
CHAPTER 13 Investigation of Septin Biology In Vivo Using
Zebrafish..............................................221
A. Willis, M. Mazon-Moya, S. Mostowy
Introduction..........................................222
1. Methods................................................226
Contents ix
Conclusion......................................... 238
Acknowledgments......................................239
References...........................................239
CHAPTER 14 Fluorescence Microscopy of Actin- and
Microtubule-Associated Septins in Mammalian
Cells................................................243
E.T. Spiliotis, E.P. Karasmanis, L. Dolat
Introduction....................................... 244
1. Methods..............................................247
2. Materials............................................259
Acknowledgments......................................263
References...........................................264
CHAPTER 15 Immunofluorescent Staining of Septins in
Primary Cilia........................................269
M.S. Kim, C.D. Froese, H. Xie, W.S. Trimble
Introduction.........................................270
1. Materials............................................271
2. Methods and Results..................................272
3. Summary.............................................281
Acknowledgments.................................... 282
References...........................................282
CHAPTER 16 Methods for Immunoblot Detection and Electron
Microscopic Localization of Septin Subunits in
Mammalian Nervous Systems...........................................285
L. K. Parajuli, N. Ageta-lshihara, H. Ageta, Y. Fukazawa,
M. Kinoshita
Introduction.........................................286
1. Methods..............................................286
Conclusion...........................................294
References...........................................294
CHAPTER 17 Visualizing Septin and Cell Dynamics in
Mammalian Brain Slices...............................295
H. Ito, R. Morishita, H. Tabata, K. Nagata
Introduction.........................................296
1. Methods..............................................298
Conclusion...........................................308
X
Contents
Acknowledgments......................................... 308
References................................................309
CHAPTER 18 Small Molecule Perturbations of Septins..................311
L.R. Heasley, M.A. McMurray
1. Forchlorfenuron...........................................311
2. l-Ethyl-3-(4-methoxyphenyl)-6-methylpyrimido[5,4-e]
[l,2,4]triazine-5,7-dione................................316
3. Summary and Perspective..................................317
References................................................317
CHAPTER 19 Septin Crystallization for Structural Analysis...........321
N.F. Valadares, R.C. Garratt
Introduction..............................................322
1. Septins and Their Structures..............................323
2. Methods..................................................326
3. General Septin Expression, Purification, and Crystallization
Protocol..................................................334
4. Final Comments...........................................336
References................................................337
Index................................................................... 339
Volumes in Series.......................................................353
|
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | First edition 2016 |
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| indexdate | 2024-07-10T07:33:03Z |
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| isbn | 9780128039984 |
| language | English |
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| physical | XV, 365 Seiten, 19 ungezählte Seiten Illustrationen |
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| series | Methods in cell biology |
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| spelling | Septins edited by Amy S. Gladfelter First edition 2016 Amsterdam Elsevier 2016 XV, 365 Seiten, 19 ungezählte Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in cell biology volume 136 Zellteilung (DE-588)4190668-8 gnd rswk-swf Strukturanalyse (DE-588)4183787-3 gnd rswk-swf Membrantransport (DE-588)4038575-9 gnd rswk-swf GTP-bindende Proteine (DE-588)4248354-2 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf GTP-bindende Proteine (DE-588)4248354-2 s Strukturanalyse (DE-588)4183787-3 s DE-604 Proteine (DE-588)4076388-2 s Zellteilung (DE-588)4190668-8 s Membrantransport (DE-588)4038575-9 s b DE-604 Gladfelter, Amy S. edt Methods in cell biology volume 136 (DE-604)BV002534878 136 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029119139&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Septins Methods in cell biology Zellteilung (DE-588)4190668-8 gnd Strukturanalyse (DE-588)4183787-3 gnd Membrantransport (DE-588)4038575-9 gnd GTP-bindende Proteine (DE-588)4248354-2 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4190668-8 (DE-588)4183787-3 (DE-588)4038575-9 (DE-588)4248354-2 (DE-588)4076388-2 |
| title | Septins |
| title_auth | Septins |
| title_exact_search | Septins |
| title_full | Septins edited by Amy S. Gladfelter |
| title_fullStr | Septins edited by Amy S. Gladfelter |
| title_full_unstemmed | Septins edited by Amy S. Gladfelter |
| title_short | Septins |
| title_sort | septins |
| topic | Zellteilung (DE-588)4190668-8 gnd Strukturanalyse (DE-588)4183787-3 gnd Membrantransport (DE-588)4038575-9 gnd GTP-bindende Proteine (DE-588)4248354-2 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Zellteilung Strukturanalyse Membrantransport GTP-bindende Proteine Proteine |
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