The zebrafish: cellular and developmental biology: Part A Cellular biology
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Weitere Verfasser: | , , |
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
Amsterdam
Elsevier, Academic Press
2016
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Ausgabe: | Fourth edition |
Schriftenreihe: | Methods in cell biology
Volume 133 |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | xvi, 305 S., 11 ungezählte Seiten Illustrationen |
ISBN: | 9780128034750 |
Internformat
MARC
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Datensatz im Suchindex
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adam_text | Contents
Contributors.............................................................xi
Preface..................................................................xv
CHAPTER 1 Embryonic Cell Culture in Zebrafish.............................1
C. A. Ciarlo, L.l. Zon
Introduction..............................................2
1. Methods...................................................3
Conclusion...............................................10
References...............................................10
CHAPTER 2 Cellular Dissection of Zebrafish Hematopoiesis.................11
D. L. Stachura, D. Traver
Introduction.............................................12
1. Zebrafish Hematopoiesis..................................12
2. Hematopoietic Cell Transplantation.......................24
3. Enrichment of HSCs..................................... 32
4. In Vitro Culture and Differentiation of Hematopoietic
Progenitors..............................................35
Conclusions..............................................46
References...............................................46
CHAPTER 3 Second Harmonic Generation Microscopy in
Zebrafish................................................55
D.C. LeBert, i.M. Squirrel), A. Huttenlocher, K.W. Eliceiri
Introduction.............................................56
1. Materials................................................59
2. Methods................................................ 62
3. Notes....................................................66
Acknowledgments..........................................66
Supplementary Data.......................................67
References...............................................67
CHAPTER 4 Imaging Blood Vessels and Lymphatic Vessels in
the Zebrafish............................................69
H.M. Jung, S. Isogai, M. Kamei, D. Castranova, A.V. Gore,
B.M. Weinstein
Introduction.............................................70
1. Imaging Vascular Gene Expression.........................71
viii Contents
2. Nonvital Blood Vessel and Lymphatic Vessel Imaging.74
3. Vital Imaging of Blood and Lymphatic Vessels..............83
Conclusion.................................................98
References..............................................99
CHAPTER 5 An Eye on Light-sheet Microscopy...............................105
D. Kromm, T. Thumberger, J. Wittbrodt
Introduction..............................................106
History...................................................106
1. Principle Behind Selective Plane IHumination Microscopy .... 107
2. The Microscope for Your Sample or the Sample for Your
Microscope?.............................................. 114
3. Data Acquisition and Handling............................117
4. Challenges and Perspectives..............................119
Acknowledgment............................................120
References..............................................121
CHAPTER 6 Single Neuron Morphology In Vivo with Confined
Primed Conversion.........................................125
M.A. Mohr, P. Pantazis
Introduction............................................. 126
1. Photoconvertible Fluorescent Proteins.....................127
2. Confined Primed Conversion................................128
3. Unraveling Single Neuron Morphology With Confined
Primed Conversion.........................................129
Conclusion and Outlook....................................135
References................................................136
CHAPTER 7 Visualizing Retinoic Acid Morphogen Gradients .... 139
T.F. Schilling, J. Sosnik, Q. Nie
Introduction..............................................140
1. Challenges for Morphogen Gradient Studies.................140
2. Feedback Allows Retinoic Acid to Act as a Graded
Morphogen.................................................142
3. Cyp26s as Key Regulators of Retinoic Acid Gradient
Formation.................................................143
4. Visualizing the Retinoic Acid Gradient...................146
5. Crabps and Retinoic Acid Signal Robustness...............150
6. Sharpening Boundaries of Gene Expression in Response to
Retinoic Acid Gradients...................................151
7. Noise—Both Good and Bad..................................156
Contents
IX
8. Other Boundaries and Other Morphogens.................. 157
Conclusions and Perspectives............................158
Acknowledgments........................................ 159
References..............................................159
CHAPTER 8 Using Fluorescent Lipids in Live Zebrafish Larvae:
from Imaging Whole Animal Physiology to
Subcellular Lipid Trafficking..........................................165
J.L. Anderson, J.D. Carten, S.A. Farber
Introduction............................................166
The Need for Whole Animal Studies of Lipid Metabolism..166
1. Forward Genetic Screening With Fluorescent Lipids...... 168
2. Visualizing Lipid Metabolism Using BODIPY Fatty Acid
Analogs.................................................171
Summary.................................................174
Acknowledgments.........................................175
References............................................. 175
CHAPTER 9 Analysis of Cilia Structure and Function in
Zebrafish............................................. 179
E. Leventea, K. Hazime, C. Zhao, J. Malicki
Introduction........................................... 180
1. Cilia in Zebrafish Organs...............................181
2. Analytical Tools for Cilia Morphology and Motility..... 190
3. Phenotypes of Cilia Mutants in Zebrafish...............208
4. Future Directions......................................215
Acknowledgments.........................................216
References..............................................216
CHAPTER 10 Functional Calcium Imaging in Zebrafish
Lateral-line Hair Cells.................................229
Q.X. Zhang, X.J. He, H.C. Wong, K.S. Kindt
Introduction............................................230
1. Calcium Indicator Selection and Comparison..............231
2. Imaging Systems and Optimal Parameters.................237
3. Image Processing.......................................242
Summary.................................................248
Discussion..............................................248
Acknowledgments.........................................249
References..............................................249
X
Contents
CHAPTER 11 Physiological Recordings from the Zebrafish
Lateral Line...........................................253
J. Olt, A.J. Ordoobadi, W. Marcotti, J.G. Trapani
Introduction...........................................254
1. Common Methods for Lateral Line Electrophysiology......255
2. Stimulation of Neuromast Hair Cells....................258
3. Recording Microphonic Potentials.......................261
4. In Vivo Hair Cell Physiology...........................265
5. Afferent Neuron Action Currents........................270
6. Summary................................................275
Discussion.............................................275
Acknowledgments........................................276
References.............................................276
Volumes in Series....................................................281
Index................................................................295
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indexdate | 2024-07-10T07:31:58Z |
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isbn | 9780128034750 |
language | English |
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spelling | The zebrafish: cellular and developmental biology Part A Cellular biology edited by H. William Detrich, III, Monte Westerfield, Leonard I. Zon Fourth edition Amsterdam Elsevier, Academic Press 2016 xvi, 305 S., 11 ungezählte Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 133 Methods in cell biology Detrich, H. William (DE-588)1013514475 edt Westerfield, Monte edt Zon, Leonard I. edt (DE-604)BV043666261 A Methods in cell biology Volume 133 (DE-604)BV002534878 133 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029079578&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | The zebrafish: cellular and developmental biology Methods in cell biology |
title | The zebrafish: cellular and developmental biology |
title_auth | The zebrafish: cellular and developmental biology |
title_exact_search | The zebrafish: cellular and developmental biology |
title_full | The zebrafish: cellular and developmental biology Part A Cellular biology edited by H. William Detrich, III, Monte Westerfield, Leonard I. Zon |
title_fullStr | The zebrafish: cellular and developmental biology Part A Cellular biology edited by H. William Detrich, III, Monte Westerfield, Leonard I. Zon |
title_full_unstemmed | The zebrafish: cellular and developmental biology Part A Cellular biology edited by H. William Detrich, III, Monte Westerfield, Leonard I. Zon |
title_short | The zebrafish: cellular and developmental biology |
title_sort | the zebrafish cellular and developmental biology cellular biology |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=029079578&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV043666261 (DE-604)BV002534878 |
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