Methods in Virology: Volume VII.
Methods in Virology, Volume VII focuses on the methods used in virology, including radioimmunoassays, microscopy, hybridization, and mutagenesis.The selection first elaborates on monoclonal antibody techniques applied to viruses; competition radioimmunoassays for characterization of antibody reactio...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Saint Louis
Elsevier Science
2014
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| Schlagworte: | |
| Online-Zugang: | FAW01 |
| Zusammenfassung: | Methods in Virology, Volume VII focuses on the methods used in virology, including radioimmunoassays, microscopy, hybridization, and mutagenesis.The selection first elaborates on monoclonal antibody techniques applied to viruses; competition radioimmunoassays for characterization of antibody reactions to viral antigens; and enzyme immunosorbent assays in plant virology. Discussions focus on the principles of enzyme immunosorbent assay, choice of enzyme and preparation of conjugate, determination of immunoglobulin class, and maintenance and specificity testing of hybridomas. The text then elaborates on electron microscopy for the identification of plant viruses in in vitro preparations and cloning and expression of viral antigens in Escherichia coli and other microorganisms, including influenza virus, expression of foreign coding sequences in Escherichia coli, hepatitis B virus, electron microscope, immunoelectron microscopy, and imaging of nucleic acids.The manuscript takes a look at the detection and characterization of subgenomic RNA in plant viruses; exploring the gene organization of baculoviruses; and spot hybridization for detection of viroids and viruses. Topics include application to viral diseases, mapping mutuations of baculoviruses, transcriptional mapping of baculovirus genomes, and genetic mapping by blot hybridization.The selection is a valuable source of information for researchers interested in the methods employed in virology |
| Beschreibung: | Description based on publisher supplied metadata and other sources |
| Beschreibung: | 1 online resource (351 pages) |
| ISBN: | 9781483269504 9780124702073 |
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| 520 | |a Methods in Virology, Volume VII focuses on the methods used in virology, including radioimmunoassays, microscopy, hybridization, and mutagenesis.The selection first elaborates on monoclonal antibody techniques applied to viruses; competition radioimmunoassays for characterization of antibody reactions to viral antigens; and enzyme immunosorbent assays in plant virology. Discussions focus on the principles of enzyme immunosorbent assay, choice of enzyme and preparation of conjugate, determination of immunoglobulin class, and maintenance and specificity testing of hybridomas. The text then elaborates on electron microscopy for the identification of plant viruses in in vitro preparations and cloning and expression of viral antigens in Escherichia coli and other microorganisms, including influenza virus, expression of foreign coding sequences in Escherichia coli, hepatitis B virus, electron microscope, immunoelectron microscopy, and imaging of nucleic acids.The manuscript takes a look at the detection and characterization of subgenomic RNA in plant viruses; exploring the gene organization of baculoviruses; and spot hybridization for detection of viroids and viruses. Topics include application to viral diseases, mapping mutuations of baculoviruses, transcriptional mapping of baculovirus genomes, and genetic mapping by blot hybridization.The selection is a valuable source of information for researchers interested in the methods employed in virology | ||
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| discipline | Biologie |
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| spelling | Maramorosch, Karl Verfasser aut Methods in Virology Volume VII. Saint Louis Elsevier Science 2014 © 1984 1 online resource (351 pages) txt rdacontent c rdamedia cr rdacarrier Description based on publisher supplied metadata and other sources Methods in Virology, Volume VII focuses on the methods used in virology, including radioimmunoassays, microscopy, hybridization, and mutagenesis.The selection first elaborates on monoclonal antibody techniques applied to viruses; competition radioimmunoassays for characterization of antibody reactions to viral antigens; and enzyme immunosorbent assays in plant virology. Discussions focus on the principles of enzyme immunosorbent assay, choice of enzyme and preparation of conjugate, determination of immunoglobulin class, and maintenance and specificity testing of hybridomas. The text then elaborates on electron microscopy for the identification of plant viruses in in vitro preparations and cloning and expression of viral antigens in Escherichia coli and other microorganisms, including influenza virus, expression of foreign coding sequences in Escherichia coli, hepatitis B virus, electron microscope, immunoelectron microscopy, and imaging of nucleic acids.The manuscript takes a look at the detection and characterization of subgenomic RNA in plant viruses; exploring the gene organization of baculoviruses; and spot hybridization for detection of viroids and viruses. Topics include application to viral diseases, mapping mutuations of baculoviruses, transcriptional mapping of baculovirus genomes, and genetic mapping by blot hybridization.The selection is a valuable source of information for researchers interested in the methods employed in virology Virology -- Technique Viruses Koprowski, Hilary Sonstige oth Erscheint auch als Druck-Ausgabe Maramorosch, Karl Methods in Virology : Volume VII |
| spellingShingle | Maramorosch, Karl Methods in Virology Volume VII. Virology -- Technique Viruses |
| title | Methods in Virology Volume VII. |
| title_auth | Methods in Virology Volume VII. |
| title_exact_search | Methods in Virology Volume VII. |
| title_full | Methods in Virology Volume VII. |
| title_fullStr | Methods in Virology Volume VII. |
| title_full_unstemmed | Methods in Virology Volume VII. |
| title_short | Methods in Virology |
| title_sort | methods in virology volume vii |
| title_sub | Volume VII. |
| topic | Virology -- Technique Viruses |
| topic_facet | Virology -- Technique Viruses |
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