G protein-coupled receptors: signaling, trafficking and regulation
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam
Elsevier
2016
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in cell biology
Volume 132 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xix, 480 Seiten, 18 ungezählte Seiten Illustrationen |
| ISBN: | 9780128035955 |
Internformat
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Datensatz im Suchindex
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| adam_text | Contents
Contributors...................................................xiii
Preface........................................................xxi
SECTION I TRAFFICKING, LOCALIZATION AND IMAGING
OF GPCRs
CHAPTER 1 Localization and Signaling of GPCRs in Lipid Rafts... 3
Van Anthony M. Vi liar, Santiago Cuevas, Xiaoxu Zheng,
Pedro A. Jose
Introduction...............................................4
1. Localization of GPCRs in Lipid Rafts......................6
2. GPCR Signaling in Lipid Rafts............................15
References................................................18
CHAPTER 2 Imaging GPCRs Trafficking and Signaling with
Total Internal Reflection Fluorescence Microscopy
in Cultured Neurons.......................................25
Francheska Delgado-Peraza, Carlos Nogueras-Ortiz,
Agnes M. Acevedo Canabal, Cristina Roman-Vendrell,
Guillermo A. Yudowski
1. Image Acquisition........................................27
2. Image Analysis...........................................30
3. Final Comments...........................................31
Acknowledgments...........................................31
References................................................31
CHAPTER 3 Trafficking of Ciliary G protein-Coupled Receptors...35
Jeremy C. McIntyre, Mellisa M. Hege, Nicolas F. Berbari
Introduction..............................................36
1. Rhodopsin as a Ciliary GPCR..............................38
2. Ciliary GPCRs in Other Neurons...........................39
3. Olfactory Sensory Neuron Ciliary GPCRs...................41
4. Dynamic Trafficking of Ciliary GPCRs.....................45
Conclusions and Future Directions.........................47
References................................................48
V
VI
Contents
CHAPTER 4 Single-molecule Resolution of G protein-Coupled
Receptor (GPCR) Complexes...............................55
Kim C. Jonas, llpo Huhtaniemi, Aylin C. Hanyaloglu
Introduction............................................56
1. Imaging Methodologies to Study Single GPCR Molecules...57
2. Methods for Photoactivated Dye-Localization Microscopy.61
Conclusions and Perspectives............................67
Acknowledgments.........................................68
References..............................................68
CHAPTER 5 Quantification of the mRNA Expression of
G protein-Coupled Receptors in Human Adipose
Tissue..................................................73
Stefan Amisten
Introduction............................................74
1. Materials...............................................74
2. Methods.................................................76
Notes..................................................100
References.............................................104
SECTION II SIGNALING AND REGULATION OF GPCRs
CHAPTER 6 Studying the Regulation of Endosomal cAMP
Production in GPCR Signaling...........................109
Alexandre Gidon, Timothy N. Feinstein, Kunhong Xiao,
Jean-Pierre Vilardaga
Introduction...........................................110
1- Materials..............................................112
2. Experimental Procedures................................114
3. Discussion.............................................124
Acknowledgments........................................125
References.............................................125
CHAPTER 7 Olfactory receptor signaling................................127
Gabriela Antunes, Fabio Marques Simoes de Souza
Introduction...........................................127
1. Specificity............................................129
2. Dimerization...........................................130
3. Perception.............................................130
4. Olfactory Transduction.................................131
Contents vii
5. Mechanisms of Odorant Adaptation......................133
6. Perspectives..........................................135
References............................................135
CHAPTER 8 Assessing Smoothened-mediated Hedgehog
signaling in zebrafish................................147
Teresa Casar Tena, Melanie Philipp
Introduction..........................................148
Hedgehog Signaling....................................148
Hedgehog-Dependent Organ Development..................149
1. Analysis of Smoothened-mediated Hh Signaling in
Zebrafish.............................................150
Summary...............................................160
Acknowledgments.......................................160
References............................................160
CHAPTER 9 GPCRs and actin-cytoskeleton dynamics......................165
Genaro Vazquez-Victorio, Claudia Gonzalez-Espinosa,
Zyanya P. Espinosa-Riquer, Marina Macias-Silva
Introduction..........................................166
1. GPCRs and G proteins..................................167
2. Actin—cytoskeleton Dynamics...........................169
3. GPCR/G Protein Signaling and Actin Polymerization.... 171
4. Cellular Processes Controlled by Actin-cytoskeleton
Rearrangements Induced by GPCRs.......................173
5. The Cross Talk of GPCR Signaling with the Homeostatic
Hippo and TGF-P Signaling Pathways....................177
6. Protocols to Evaluate Actin-cytoskeleton Rearrangements.... 177
7. Method to Evaluate the Actin-cytoskeleton Dynamics
Modulated by GPCR Signaling in Hepatic Cells..........178
8. Protocols for IF Assays...............................179
Conclusions...........................................184
Acknowledgments.......................................184
References............................................185
SECTION III CELLULAR ASSAYS FOR GPCRs
CHAPTER 10 GPCR-radioligand binding assays......................191
Colleen A. Flanagan
1. Types of Binding Assays...........................192
viii Contents
2. Components of Binding Assays—Considerations for GPCR
Binding Assay Design...................................202
Abbreviations..........................................212
Acknowledgments........................................212
References.............................................212
CHAPTER 11 Tracking GPCR Biosynthesis and Degradation
Using a Nonradioactive Pulse Chase
Methodology...........................................................217
Richard Wargachuk, Dominic Devost, Cynthia Zhou,
Terence E. Hébert
Introduction...........................................218
1. Materials Required....................................219
2. Basic Methodology.....................................220
3. General Workflow of Experiments.......................223
4. Results...............................................223
5. Discussion............................................227
Acknowledgments........................................230
References.............................................230
CHAPTER 12 Tango Assay for Ligand-induced GPCR-
0-arrestin2 Interaction: Application in
Drug Discovery.........................................233
Shalini Dogra, Chandan Sona, Ajeet Kumar, Prem N. Yadav
1. Background and Rationale..............................234
2. Various 3-Arrestin Recruitment Assays.................236
3. Principle of Tango Assay..............................239
4. Reagents and Methods..................................240
5. Data Analysis.........................................245
6. Results and Data Interpretation.......................247
7. Summary and Future Perspectives.......................250
Acknowledgments........................................252
References.............................................252
CHAPTER 13 Resonance Energy Transfer-based Approaches
to Study GPCRs.........................................255
Mohammed Akli Ayoub
Introduction...........................................256
1. RET Approaches........................................257
2. Ligand Binding........................................258
Contents ix
3. Ligand-Induced Conformational Changes..................259
4. GPCR—G protein Interaction.............................261
5. Second Messenger Production............................265
6. P-Arrestin Recruitment.................................266
7. Receptor Internalization...............................269
8. Receptor Oligomerization...............................270
9. Summary and Outlook....................................277
Acknowledgments.........................................277
References..............................................277
CHAPTER 14 Quantitative Analysis of G-protein-Coupled
Receptor Internalization Using DnaE
Intein-based Assay......................................293
Bin Lu, Linjie Chen, Yaping Zhang, Ying Shi, Naiming Zhou
Introduction............................................294
1. Materials and Methods...................................296
2. Discussion.............................................311
Summary.................................................313
Acknowledgments.........................................313
References..............................................314
CHAPTER 15 Cellular and Subcellular Context Determine
Outputs from Signaling Biosensors.......................319
Dominic Devost, Nicolas Audet, Cynthia Zhou,
Hiroyuki Kobayashi, Hélène Bonin, Viktorya Lukashova,
Christian Le Gouill, Michel Bouvier, Terence E. Hébert
Introduction and Rationale..............................320
Introduction............................................320
Rationale and Objectives................................321
1. Materials and Instrumentation Required.................322
2. Basic Methodology......................................323
3. Experimental Strategies................................326
4. Discussion.............................................335
Acknowledgments.........................................335
References..............................................336
X
Contents
SECTION IV STRUCTURAL AND COMPUTATIONAL
INVESTIGATION OF GPCRs
CHAPTER 16 Protease-activated Receptors (PARs) in
Cancer: Novel Biased Signaling and Targets
for Therapy............................................341
R. Bar-Shavit, M. Maoz, A. Kancharla, M. Jaber,
D. Agranovich, S. Grisaru-Granovsky, B. Uziely
Introduction...........................................342
1. Novel Signaling of PARs Endowing Critical PH-Domain-
Binding Motifs.........................................346
2. PARs and Human Placenta...............................350
3. Future Directions.....................................353
Acknowledgments........................................353
References.............................................353
CHAPTER 17 Computational Methods for Studying
G protein-Coupled Receptors (GPCRs)....................359
Agnieszka A. Kaczor, Ewelina Rutkowska, Damian Bartuzi,
Katarzyna M. Targowska-Duda, Dariusz Matosiuk,
Jana Selent
Introduction...........................................360
1. Prediction of Receptor Structure......................362
2. Studies of Ligand—Receptor Interactions...............364
3. Biased Signaling......................................372
4. Allosterism...........................................376
5. Dimerization..........................................380
Acknowledgments........................................385
References.............................................385
CHAPTER 18 Comparing Class A GPCRs to Bitter Taste
Receptors: Structural Motifs, Ligand Interactions
and Agonist-to-antagonist Ratios.......................401
Antonella Di Pizio, Anat Levit, Michal Slutzki,
Maik Behrens, Rafik Karaman, Masha Y. Niv
Introduction...........................................402
1. Sequence Is Less Conserved Than Structure: Sequence
Alignments and GPCR Signature Motifs...................404
2. Ligand—Receptor Interactions in TAS2Rs Compared
to Class A GPCRs.......................................410
Contents xi
Conclusion and Outlook...................................420
Acknowledgments..........................................420
References...............................................420
CHAPTER 19 What Can Simulations Tell Us About GPCRs:
Integrating the Scales...................................429
Durba Sengupta, Manali Joshi, Chaitanya A. Athale,
Amitabha Chattopadhyay
Introduction.............................................430
1. Multiscale Simulations: Accuracy, Advantages, and
Limitations..............................................432
2. Analyzing the Dynamics of GPCR—Ligand Interactions.....437
3. Can Current Simulation Techniques Allow Us to Study
GPCR Activation ?........................................441
4. The Dynamic Interplay between the Membrane
and Receptors............................................443
5. GPCR Organization in the Cell Membrane..................446
Conclusions..............................................447
Acknowledgments..........................................447
References...............................................447
Volumes in Series.......................................................453
Index...................................................................467
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| spelling | G protein-coupled receptors signaling, trafficking and regulation edited by Arun K. Shukla, Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India First edition Amsterdam Elsevier 2016 xix, 480 Seiten, 18 ungezählte Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in cell biology Volume 132 Methode (DE-588)4038971-6 gnd rswk-swf G-Protein gekoppelter Rezeptor (DE-588)7723480-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content G-Protein gekoppelter Rezeptor (DE-588)7723480-7 s Methode (DE-588)4038971-6 s b DE-604 Shukla, Arun K. 1981- (DE-588)131837397 edt Methods in cell biology Volume 132 (DE-604)BV002534878 132 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028987151&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | G protein-coupled receptors signaling, trafficking and regulation Methods in cell biology Methode (DE-588)4038971-6 gnd G-Protein gekoppelter Rezeptor (DE-588)7723480-7 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)7723480-7 (DE-588)4143413-4 |
| title | G protein-coupled receptors signaling, trafficking and regulation |
| title_auth | G protein-coupled receptors signaling, trafficking and regulation |
| title_exact_search | G protein-coupled receptors signaling, trafficking and regulation |
| title_full | G protein-coupled receptors signaling, trafficking and regulation edited by Arun K. Shukla, Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India |
| title_fullStr | G protein-coupled receptors signaling, trafficking and regulation edited by Arun K. Shukla, Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India |
| title_full_unstemmed | G protein-coupled receptors signaling, trafficking and regulation edited by Arun K. Shukla, Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India |
| title_short | G protein-coupled receptors |
| title_sort | g protein coupled receptors signaling trafficking and regulation |
| title_sub | signaling, trafficking and regulation |
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