Directed evolution of selective enzymes: catalysts for organic chemistry and biotechnology
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Weinheim
Wiley-VCH Verlag GmbH & Co. KGaA
[2017]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 308 Seiten Illustrationen, Diagramme |
| ISBN: | 9783527316601 |
Internformat
MARC
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| 245 | 1 | 0 | |a Directed evolution of selective enzymes |b catalysts for organic chemistry and biotechnology |c Manfred T. Reetz |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-028985723 | ||
Datensatz im Suchindex
| _version_ | 1804176246526967808 |
|---|---|
| adam_text | CONTENTS
I
1.1
1.2
1.3
2
2.1
2.2
2.3
3
3.1
3.2
3.3
3.4
3.5
3.6
3.7
4
4.1
4.2
4.3
4.3.1
PREFACE IX
INTRODUCTION TO DIRECTED EVOLUTION 1
GENERAL DEFINITION AND PURPOSE OF DIRECTED EVOLUTION OF
ENZYMES 1
BRIEF ACCOUNT OF THE HISTORY OF DIRECTED EVOLUTION 4
APPLICATIONS OF DIRECTED EVOLUTION OF ENZYMES 16
REFERENCES 17
SELECTION VERSUS SCREENING IN DIRECTED EVOLUTION 27
SELECTION SYSTEMS 27
SCREENING SYSTEMS 44
CONCLUSIONS AND PERSPECTIVES 52
REFERENCES S3
GENE MUTAGENESIS METHODS 59
INTRODUCTORY REMARKS 59
ERROR-PRONE POLYMERASE CHAIN REACTION (EPPCR) AND OTHER
WHOLE-GENE MUTAGENESIS TECHNIQUES 60
SATURATION MUTAGENESIS: AWAY FROM BLIND DIRECTED EVOLUTION 70
RECOMBINANT GENE MUTAGENESIS METHODS 85
CIRCULAR PERMUTATION AND OTHER DOMAIN SWAPPING TECHNIQUES 91
SOLID-PHASE COMBINATORIAL GENE SYNTHESIS FOR LIBRARY CREATION 92
COMPUTATIONAL TOOLS 96
REFERENCES 101
STRATEGIES FOR APPLYING GENE MUTAGENESIS METHODS 115
GENERAL GUIDELINES 115
RARE CASES OF COMPARATIVE STUDIES 118
CHOOSING THE BEST STRATEGY WHEN APPLYING SATURATION
MUTAGENESIS 130
GENERAL GUIDELINES 130
4.3.2
4.3.3
4.3.4
4.3.5
4.4
4.5
5
5.1
5.2
6
6.1
6.2
6.3
6.4
6.5
6.6
6
.
6.1
6
.
6.2
6.6.3
6.6.4
6.6.5
7
7.1
7.2
7.3
CHOOSING OPTIMAL PATHWAYS IN ITERATIVE SATURATION MUTAGENESIS
(ISM) 135
SYSTEMATIZATION OF SATURATION MUTAGENESIS 142
SINGLE CODE SATURATION MUTAGENESIS (SCSM): USE OF A SINGLE AMINO
ACID AS BUILDING BLOCK 149
TRIPLE CODE SATURATION MUTAGENESIS (TCSM): A VIABLE
COMPROMISE WHEN CHOOSING THE OPTIMAL REDUCED AMINO ACID
ALPHABET 151
TECHNO-ECONOMICAL ANALYSES OF SATURATION MUTAGENESIS
STRATEGIES 154
COMBINATORIAL SOLID-PHASE GENE SYNTHESIS: AN ALTERNATIVE FOR
THE FUTURE? 159
REFERENCES 160
SELECTED EXAMPLES OF DIRECTED EVOLUTION OF ENZYMES WITH
EMPHASIS ON STEREO- AND REGIOSELECTIVITY, SUBSTRATE SCOPE, AND/OR
ACTIVITY 167
EXPLANATORY REMARKS 167
COLLECTION OF SELECTED EXAMPLES FROM THE LITERATURE 2010 UP TO
2016 189
REFERENCES 189
DIRECTED EVOLUTION OF ENZYME ROBUSTNESS 205
INTRODUCTION 205
APPLICATION OF EPPCR AND DNA SHUFFLING 207
B-FIT APPROACH 211
ITERATIVE SATURATION MUTAGENESIS (ISM) AT PROTEIN-PROTEIN
INTERFACIAL SITES FOR MULTIMERIC ENZYMES 215
ANCESTRAL AND CONSENSUS APPROACHES AND THEIR STRUCTURE-GUIDED
EXTENSIONS 216
COMPUTATIONALLY GUIDED METHODS 219
SCHEMA APPROACH 219
FRESCO APPROACH 221
FIREPROT APPROACH 223
CONSTRAINED NETWORK ANALYSIS (CNA) APPROACH 224
ALTERNATIVE APPROACHES 226
REFERENCES 227
DIRECTED EVOLUTION OF PROMISCUITY: ARTIFICIAL ENZYMES AS CATALYSTS
IN ORGANIC CHEMISTRY 237
INTRODUCTORY BACKGROUND INFORMATION 237
TUNING THE CATALYTIC PROFILE OF PROMISCUOUS ENZYMES BY
DIRECTED EVOLUTION 245
CONCLUSIONS AND PERSPECTIVES 259
REFERENCES
260
8
8.1
8.2
8.2.1
8
.
2.2
8.2.3
8.2.4
8.3
LEARNING FROM DIRECTED EVOLUTION 267
BACKGROUND INFORMATION 267
CASE STUDIES FEATURING MECHANISTIC, STRUCTURAL, AND/OR
COMPUTATIONAL ANALYSES OF THE SOURCE OF EVOLVED STEREO- AND/OR
REGIOSELECTIVITY
269
EPOXIDE HYDROLASE 269
ENE-REDUCTASE OF THE OLD YELLOW ENZYME (OYE) 273
ESTERASE 279
CYTOCHROME P450 MONOOXYGENASE 282
ADDITIVE VERSUS NON-ADDITIVE MUTATIONAL EFFECTS IN FITNESS
LANDSCAPES 287
REFERENCES 296
INDEX 303
|
| any_adam_object | 1 |
| author | Reetz, Manfred T. 1943- |
| author_GND | (DE-588)111053455 |
| author_facet | Reetz, Manfred T. 1943- |
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| author_variant | m t r mt mtr |
| building | Verbundindex |
| bvnumber | BV043570858 |
| classification_rvk | VK 8700 VN 8950 WC 4400 WD 5050 |
| ctrlnum | (OCoLC)965604229 (DE-599)DNB109909903X |
| dewey-full | 540 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 540 - Chemistry and allied sciences |
| dewey-raw | 540 |
| dewey-search | 540 |
| dewey-sort | 3540 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T07:29:08Z |
| institution | BVB |
| isbn | 9783527316601 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-028985723 |
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| physical | IX, 308 Seiten Illustrationen, Diagramme |
| publishDate | 2017 |
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| publisher | Wiley-VCH Verlag GmbH & Co. KGaA |
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| spelling | Reetz, Manfred T. 1943- Verfasser (DE-588)111053455 aut Directed evolution of selective enzymes catalysts for organic chemistry and biotechnology Manfred T. Reetz Weinheim Wiley-VCH Verlag GmbH & Co. KGaA [2017] © 2017 IX, 308 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Organische Synthese (DE-588)4075695-6 gnd rswk-swf Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Organische Synthese (DE-588)4075695-6 s Enzymkatalyse (DE-588)4152480-9 s DE-604 Erscheint auch als Online-Ausgabe, PDF 978-3-527-65549-6 Erscheint auch als Online-Ausgabe, EPUB 978-3-527-65548-9 Erscheint auch als Online-Ausgabe, MOBI 978-3-527-65547-2 Erscheint auch als Online-Ausgabe, oBook 978-3-527-65546-5 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028985723&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Reetz, Manfred T. 1943- Directed evolution of selective enzymes catalysts for organic chemistry and biotechnology Organische Synthese (DE-588)4075695-6 gnd Enzymkatalyse (DE-588)4152480-9 gnd |
| subject_GND | (DE-588)4075695-6 (DE-588)4152480-9 |
| title | Directed evolution of selective enzymes catalysts for organic chemistry and biotechnology |
| title_auth | Directed evolution of selective enzymes catalysts for organic chemistry and biotechnology |
| title_exact_search | Directed evolution of selective enzymes catalysts for organic chemistry and biotechnology |
| title_full | Directed evolution of selective enzymes catalysts for organic chemistry and biotechnology Manfred T. Reetz |
| title_fullStr | Directed evolution of selective enzymes catalysts for organic chemistry and biotechnology Manfred T. Reetz |
| title_full_unstemmed | Directed evolution of selective enzymes catalysts for organic chemistry and biotechnology Manfred T. Reetz |
| title_short | Directed evolution of selective enzymes |
| title_sort | directed evolution of selective enzymes catalysts for organic chemistry and biotechnology |
| title_sub | catalysts for organic chemistry and biotechnology |
| topic | Organische Synthese (DE-588)4075695-6 gnd Enzymkatalyse (DE-588)4152480-9 gnd |
| topic_facet | Organische Synthese Enzymkatalyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028985723&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT reetzmanfredt directedevolutionofselectiveenzymescatalystsfororganicchemistryandbiotechnology |