Plant proteostasis: methods and protocols
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
[2016]
|
| Schriftenreihe: | Methods in molecular biology
1450 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xi, 307 Seiten Illustrationen, Diagramme |
| ISBN: | 9781493937578 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804176243282673664 |
|---|---|
| adam_text | Titel: Plant proteostasis
Autor: Lois, L. Maria
Jahr: 2016
Contents
Preface.......................................................... v
Contributors.......................................................... ix
Part I Ubiquitin Conjugation and Deconjugation Analysis
1 Approaches to Determine Protein Ubiquitination Residue Types........... 3
Qian Chen, Xiaoyuan Tang, and Qi Xie
2 Immunoprecipitation of Cullin-Ring Ligases (CRLs) in Arabidopsis
thaliana Seedlings.............................................. 11
Anna Franciosini and Giovanna Serino
3 Radioligand Binding Assays for Determining Dissociation Constants
of Phytohormone Receptors...................................... 23
Antje Hellmuth and Luz Irina A. Calderon Villalobos
4 Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes.... 35
Kamila Kalinowska, Marie-Kristin Nagel, and Erika Isono
5 Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants....... 45
Katarzyna Zientara-Rytter and Agnieszka Sirko
6 Generation of Artificial N-end Rule Substrate Proteins
In Vivo and In Vitro............................................ 55
Christin Naumann, Augnstin C. Mot, and Nico Dissmeyer
7 Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases............... 85
Maria Klecker and Nico Dissmeyer
Part II Ubiquitin-like Protein Conjugation, Deconjugation,
and Cell Imaging Studies
8 SUMO Chain Formation by Plant Enzymes.......................... 97
Konstantin Tomanov, Ionida Ziba, and Andreas Bachmair
9 Kinetic Analysis of Plant SUMO Conjugation Machinery................ 107
Laura Castano-Miquel and L. Maria Lois
10 Expression, Purification, and Enzymatic Analysis
of Plant SUMO Proteases........................................ 125
Gary Yates, Anjil Srivastava, Beatriz Orosa, and Ari Sadanandom
11 Quantitative Analysis of Subcellular Distribution of the SUMO
Conjugation System by Confocal Microscopy Imaging.................. 135
Abraham Mas, Montse Amends, and L. Maria Lois
12 Biochemical Analysis of Autophagy in Algae and Plants
by Monitoring the Electrophoretic Mobility of ATG8................... 151
Maria Esther Perez-Perez, Ascension Andres-Garrido,
and Josi L. Crespo
vii
viii Contents
13 Detection of Autophagy in Plants by Fluorescence Microscopy............ 161
Hunting Pu and Diane C. Bassham
Part III Proteomic Analysis and Other Post-Translational
Modification Studies
14 Protocols for Studying Protein Stability in an Arabidopsis
Protoplast Transient Expression System.............................. 175
Severine Planchais, Laurent Camborde, and Isabelle Jupin
15 Detection and Quantification of Protein Aggregates in Plants............. 195
Marc Planas-Marques, Saul Lema A., and Nuria S. Coll
16 Determination of Protein Carbonylation and Proteasome
Activity in Seeds............................................... 205
QiongXia, Hayat El-Maarouf-Bouteau, Christophe Bailly,
and Patrice Meimoun
17 Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-Based
Protein Profiling in Plants........................................ 213
Isabel Cristina Velez-Bermudez, Tuan-Nan Wen, Ping Lan,
and Wolfgang Schmidt
18 Use of a Phosphatidylinositol Phosphate Affinity Chromatography
(PIP Chromatography) for the Isolation of Proteins Involved
in Protein Quality Control and Proteostasis Mechanisms in Plants.......... 223
T. Farmaki
19 In Vivo Radiolabeling of Arabidopsis Chloroplast Proteins and
Separation of Thylakoid Membrane Complexes by Blue Native PAGE....... 233
Catharina Nickel, Thomas Brylok, and Serena Schwenkert
20 Normalized Quantitative Western Blotting Based on Standardized
Fluorescent Labeling............................................ 247
Frederik Faden, Lennart Eschen-Lippold, and Nico Dissmeyer
Part IV Bioinformatics Analysis
21 Sequence Search and Comparative Genomic Analysis of SUMO-Activating
Enzymes Using CoGe........................................... 261
Lorenzo Carretero-Paulet and Victor A, Albert
22 Studying Evolutionary Dynamics of Gene Families Encoding
SUMO-Activating Enzymes with SeaView and ProtTest................. 273
Lorenzo Carretero-Paulet and Victor A. Albert
23 Bioinformatics Tools for Exploring the SUMO Gene Network............ 285
Pedro Humberto Castro, Miguel Angelo Santos, Alexandre Papadopoulos
Magalhaes, Rui Manuel Tavares, and Herlander Azevedo
Index............................................................... 303
|
| any_adam_object | 1 |
| author2 | Lois, L. Maria Matthiesen, Rune |
| author2_role | edt edt |
| author2_variant | l m l lm lml r m rm |
| author_facet | Lois, L. Maria Matthiesen, Rune |
| building | Verbundindex |
| bvnumber | BV043568773 |
| classification_rvk | WN 5400 |
| ctrlnum | (OCoLC)956973735 (DE-599)BVBBV043568773 |
| dewey-full | 581.7 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 581 - Specific topics in natural history of plants |
| dewey-raw | 581.7 |
| dewey-search | 581.7 |
| dewey-sort | 3581.7 |
| dewey-tens | 580 - Plants |
| discipline | Biologie |
| format | Book |
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| spelling | Plant proteostasis methods and protocols edited by Rune Matthiesen, L. Maria Lois New York Humana Press [2016] xi, 307 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1450 Springer protocols Stressreaktion (DE-588)4138565-2 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Homöostase (DE-588)4160575-5 gnd rswk-swf Proteom (DE-588)4576155-3 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Ubiquitin (DE-588)4314848-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzen (DE-588)4045539-7 s Stressreaktion (DE-588)4138565-2 s Ubiquitin (DE-588)4314848-7 s Proteom (DE-588)4576155-3 s Homöostase (DE-588)4160575-5 s Molekularbiologie (DE-588)4039983-7 s DE-604 Lois, L. Maria edt Matthiesen, Rune edt Erscheint auch als Online-Ausgabe 978-1-4939-3759-2 Methods in molecular biology 1450 (DE-604)BV035362695 1450 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028983693&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Plant proteostasis methods and protocols Methods in molecular biology Stressreaktion (DE-588)4138565-2 gnd Molekularbiologie (DE-588)4039983-7 gnd Homöostase (DE-588)4160575-5 gnd Proteom (DE-588)4576155-3 gnd Pflanzen (DE-588)4045539-7 gnd Ubiquitin (DE-588)4314848-7 gnd |
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| title | Plant proteostasis methods and protocols |
| title_auth | Plant proteostasis methods and protocols |
| title_exact_search | Plant proteostasis methods and protocols |
| title_full | Plant proteostasis methods and protocols edited by Rune Matthiesen, L. Maria Lois |
| title_fullStr | Plant proteostasis methods and protocols edited by Rune Matthiesen, L. Maria Lois |
| title_full_unstemmed | Plant proteostasis methods and protocols edited by Rune Matthiesen, L. Maria Lois |
| title_short | Plant proteostasis |
| title_sort | plant proteostasis methods and protocols |
| title_sub | methods and protocols |
| topic | Stressreaktion (DE-588)4138565-2 gnd Molekularbiologie (DE-588)4039983-7 gnd Homöostase (DE-588)4160575-5 gnd Proteom (DE-588)4576155-3 gnd Pflanzen (DE-588)4045539-7 gnd Ubiquitin (DE-588)4314848-7 gnd |
| topic_facet | Stressreaktion Molekularbiologie Homöostase Proteom Pflanzen Ubiquitin Aufsatzsammlung |
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