Chemokines:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA
Academic Press
2016
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in enzymology
volume 570 |
| Schlagworte: | |
| Online-Zugang: | TUM01 UBM01 UBR01 UBT01 UBW01 UER01 URL des Erstveröffentlichers Inhaltsverzeichnis |
| Beschreibung: | 1 Online-Ressource |
| ISBN: | 9780128021712 9780128021958 |
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Datensatz im Suchindex
| _version_ | 1804176010636165120 |
|---|---|
| adam_text | Titel: Chemokines
Autor: Handel, Tracy M
Jahr: 2016
CONTENTS
Contributors xiii
Preface xxi
1.Chemokine Detection Using Receptors Immobilized on an SPR
Sensor Surface 1
Jose Miguel Rodrfguez-Frade, Laura Marti nez-Munoz, Ricardo Villares,
Graciela Cascio, Pilar Lucas, Rosa P. Gomariz, and Mario Mellado
1. Surface Plasmon Resonance 2
2. Chemokine Receptors: Members of the GPCR Family S
3. Chemokine Receptor Immobilization on the Sensor Chip 4
4. Viral Particles as Chemokine Receptors Carriers in SPR 6
5. SPR-Based Applications for Chemokine Receptors 12
6. Conclusions 14
Acknowledgments 15
References 15
2. Study of Chemotaxis and Cell-Cell Interactions in Cancer with
Microfluidic Devices 19
Jiqing Sai, Matthew Rogers, Kathryn Hockemeyer, John P. Wikswo,
and Ann Richmond
1. Introduction 20
2. Methods 24
3. Limitations 37
4. Perspectives 40
Acknowledgments 42
References 43
3. Generating Chemokine Analogs with Enhanced Pharmacological
Properties Using Phage Display 47
Karim Dorgham, Fabrice Cerini, Flubert Gaertner, Astrid Melotti,
Irene Rossitto-Borlat, Guy Gorochov, and Oliver Hartley
1. Introduction 48
2. Methods 50
3. Limitations 65
4. Perspectives 68
References 70
vi
Contents
4. Methods for the Recognition of GAG-Bound Chemokines 73
Pauline Bonvin, Franck Gueneau, Nicolas Fischer, and Amanda Proudfoot
1. Introduction 73
2. Methods 74
3. Summary 85
References 85
5. Monitoring Scavenging Activity of Chemokine Receptors 87
Barbara Moepps and Marcus Thelen
1. Introduction 88
2. Fluorescent Chemokines 89
3. Monitoring Scavenging with Radiolabeled Chemokines 92
4. Monitoring Scavenging with Fluorescent Chemokines 93
5. Monitor Chemokine Uptake by Microscopy and Flow Cytometry (FACS) 108
References 114
6. Dual-Color Luciferase Complementation for Chemokine
Receptor Signaling 119
Kathryn E. Luker and Gary D. Luker
1. Introduction 120
2. Methods 121
Acknowledgment 128
References 128
7. Analysis of Arrestin Recruitment to Chemokine Receptors
by Bioluminescence Resonance Energy Transfer 131
J. Bonneterre, N. Montpas, C. Boularan, C. Gales, and N. Fleveker
1. Introduction 132
2. Methods 133
3. Interpretation and Limitations of BRET Data 145
4. Perspectives 149
Acknowledgments 150
References 150
8. Probing Biased Signaling in Chemokine Receptors 155
Roxana-Maria Amarandi, Gertrud Malene Hjort0, Mette Marie Rosenkilde,
and Stefanie Karlshaj
1. Introduction 156
2. Types of Bias in the Chemokine System 157
Contents
vii
3. Chemokine System-Mediated Intracellular Signaling 162
4. Methods 165
Acknowledgments 180
References 181
9. Mutagenesis by Phage Display 187
Pauline Bonvln, Christine Power, Amanda Proudfoot, and Steven Dunn
1. Introduction 187
2. Investigating the Pharmacophore of Chemokine Binders
by Phage Display 188
3. Phage Display Selection to Modulate the Selectivity
of Chemokine Binders 197
4. Summary 205
References 205
10. Studying Chemokine Control of Neutrophil Migration In Vivo
in a Murine Model of Inflammatory Arthritis 207
Yoshishige Miyabe, Nancy D. Kim, Chie Nliyabe, and Andrew D. Luster
1. Introduction 208
2. Methods 211
3. Conclusions 229
References 230
11. Production of Chemokine/Chemokine Receptor Complexes
for Structural Biophysical Studies 233
Martin Gustavsson, Yi Zheng, and Tracy M. Handel
1. Introduction 234
2. Methods 236
3. Summary and Conclusions 257
Acknowledgments 257
References 258
12.In Vivo Models to Study Chemokine Biology 261
F.A. Amaral, D. Boff, and M.M. Teixeira
1. Introduction 262
2. Methods 264
3. Limitations 275
4. Other Perspectives 277
References 277
viii
Contents
13. Monitoring Chemokine Receptor Trafficking by Confocal
Immunofluorescence Microscopy 281
Adriano Marchese
1. Introduction 282
2. Methods 284
3. Concluding Remarks 290
References 291
14. Active Shaping of Chemokine Gradients by Atypical Chemokine
Receptors: A 4D Live-Cell Imaging Migration Assay 293
Kathrin Werth and Reinhold Forster
1. Background 294
2. Introduction 294
3. Materials and Equipment 296
4. Preparation of Chambers 297
5. Preparation of Cells 299
6. Filling of Chambers 300
7. Time-Lapse Imaging 302
8. Analysis 302
Acknowledgments 307
References 307
15. The Role of Chemokine and Glycosaminoglycan Interaction in
Chemokine-Mediated Migration In Vitro and In Vivo 309
Irene del Molino del Barrio, John Kirby, and Simi Ali
1. Introduction 310
2. In Vitro Chemotaxis 311
3. In Vivo Chemotaxis 318
4. Generation of Mammalian Transfectants Expressing Chemokine Receptors 322
5. Discussion 329
Acknowledgments 330
References 331
16. Examining Roles of Glycans in Chemokine-Mediated Dendritic-
Endothelial Cell Interactions 335
Xin Yin, Scott C. Johns, Roland El Ghazal, Catherina L. Salanga, Tracy M.
Handel, and Mark M. Fuster
1. Introduction 336
2. Methods 339
Contents
ix
3. Perspectives 350
Acknowledgments 353
References 353
17. Preparation and Analysis of N-Terminal Chemokine Receptor
Sulfopeptides Using Tyrosylprotein Sulfotransferase Enzymes 357
Christoph Seibert, Anthony Sanfiz, Thomas P. Sakmar,
and Christopher T. Veldkamp
1. Introduction 358
2. Methods 361
3. Caveats and Limitations 380
4. Perspectives 381
Acknowledgments 385
References 385
18. Disulfide Trapping for Modeling and Structure Determination
of Receptor:Chemokine Complexes 389
Irina Kufareva, Martin Gustavsson, Lauren G. Holden, Ling Qin, Yi Zheng,
and Tracy M. Handel
1. Introduction 390
2. Architecture of ReceptonChemokine Interfaces 392
3. Cysteine as a Natural Crosslinking Agent 395
4. Disulfide Trapping 396
5. Conclusion and Perspectives 416
Acknowledgments 417
References 417
19. Analysis of G Protein and 0-Arrestin Activation in Chemokine
Receptors Signaling 421
Alessandro Vacchini, Marta Busnelli, Bice Chini, Massimo Locati,
and Elena Monica Borroni
1. Introduction 422
2. G Proteins Signaling 423
3. p-Arrestins Signaling: Detection of p-Arrestins Recruitment by Protein
Conformational Changes 431
4. Calculation of Biased Signaling 434
5. Summary 436
References 437
Contents
20. Flow Cytometry Detection of Chemokine Receptors for the
Identification of Murine Monocyte and Neutrophil Subsets
Ornella Bonavita, Matteo Massara, Achille Anselmo, Paolo Somma,
Hilke Bruhl, Matthias Mack, Massimo Locati, and Raffaella Bonecchi
1. Introduction
2. Blood Collection and Preparation of Blood Cells from Mice
3. Staining of Cell Surface Markers and Chemokine Receptors
4. Flow Cytometry Analysis
5. Discussion
6. Concluding Remarks
Acknowledgments
References
21. Molecular Pharmacology of Chemokine Receptors
Raymond H. de Wit, Sabrina M. de Munnik, Rob Leurs, Henry F. Vischer,
and Martine J. Smit
1. Introduction 458
2. Pharmacological Quantification of Chemokine Receptor Binding 462
3. Pharmacological Quantification of Chemokine Receptor Signaling 482
4. Conclusions and Future Perspectives 505
Acknowledgments 507
References 507
22. Preparation and Characterization of Glycosaminoglycan
Chemokine Coreceptors 517
Nikola Kitic, Martha Gschwandtner, Rupert Derler, Tanja Gerlza,
and Andreas J. Kungl
1. Introduction 518
2. Preparation and Characterization of GAGs 520
3. Methods for Studying Chemokine-GAG Interactions 529
4. Concluding Remarks 536
Acknowledgment 537
References 537
441
442
444
445
449
453
454
455
455
457
Contents xi
23. Production of Recombinant Chemokines and Validation
of Refolding 539
Christopher T. Veldkamp, Chad A. Koplinski, Davin R. Jensen,
Francis C. Peterson, Kaitlin M. Smits, Brittney L. Smith, Scott K. Johnson,
Christina Lettieri, Wallace G. Buchholz, Joyce C. Solheim,
and Brian F. Volkman
1. Introduction 540
2. Methods 544
3. Caveats and Limitations 558
4. Perspectives 558
Acknowledgments 562
References 562
24. Quantitative Analysis of Dendritic Cell Haptotaxis 567
Jan Schwarz and Michael Sixt
1. Introduction 567
2. Methods 570
3. Perspectives 580
Acknowledgments 581
References 581
Author Index
Subject Index
583
615
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| spelling | Chemokines edited by Tracy M. Handel First edition Cambridge, MA Academic Press 2016 1 Online-Ressource txt rdacontent c rdamedia cr rdacarrier Methods in enzymology volume 570 Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Chemokine (DE-588)4429989-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Chemokine (DE-588)4429989-8 s Biochemische Analyse (DE-588)4255721-5 s DE-604 Untersuchungsmethode (DE-588)4324907-3 s Handel, Tracy M. edt Methods in enzymology volume 570 (DE-604)BV021328877 570 http://www.sciencedirect.com/science/bookseries/00766879/570 Verlag URL des Erstveröffentlichers Volltext HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028836383&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Chemokines |
| title_auth | Chemokines |
| title_exact_search | Chemokines |
| title_full | Chemokines edited by Tracy M. Handel |
| title_fullStr | Chemokines edited by Tracy M. Handel |
| title_full_unstemmed | Chemokines edited by Tracy M. Handel |
| title_short | Chemokines |
| title_sort | chemokines |
| topic | Untersuchungsmethode (DE-588)4324907-3 gnd Biochemische Analyse (DE-588)4255721-5 gnd Chemokine (DE-588)4429989-8 gnd |
| topic_facet | Untersuchungsmethode Biochemische Analyse Chemokine Aufsatzsammlung |
| url | http://www.sciencedirect.com/science/bookseries/00766879/570 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028836383&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV021328877 |
| work_keys_str_mv | AT handeltracym chemokines |