Introduction to biosensors: from electric circuits to immunosensors
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Schweiz
Springer
[2016]
|
| Ausgabe: | Second edition |
| Schlagworte: | |
| Online-Zugang: | https://doi.org/10.1007/978-3-319-27413-3 Inhaltsverzeichnis |
| Beschreibung: | xv, 331 Seiten Illustrationen, Diagramme (teilweise farbig) |
| ISBN: | 9783319274119 |
| DOI: | 10.1007/978-3-319-27413-3 |
Internformat
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| 650 | 4 | |a Biomedical engineering | |
| 650 | 4 | |a Biophysics | |
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Datensatz im Suchindex
| _version_ | 1804175881101377536 |
|---|---|
| adam_text | Titel: Introduction to biosensors
Autor: Yoon, Jeong-Yeol
Jahr: 2016
Contents
1 Introduction......................................... 1
1.1 Sensors....................................... 1
1.2 Transducers.................................... 2
1.3 Biosensors..................................... 5
1.4 Bioreceptors.................................... 6
1.5 Transducers for Biosensors......................... 12
1.6 Overview of This Textbook......................... 13
References and Further Readings........................... 15
2 Resistors........................................... 17
2.1 Electric Circuit.................................. 17
2.2 Current and Voltage.............................. 17
2.3 Resistance and Ohm s Law......................... 19
2.4 Resistors in Series, or Voltage Divider................. 20
2.5 Potentiometer, or Pot............................. 22
2.6 Resistors in Parallel, or Current Divider................ 23
2.7 Reading Resistor Values........................... 24
2.8 Breadboards.................................... 26
2.9 Laboratory Task 1: Resistors in Series.................. 27
2.10 Laboratory Task 2: Resistors in Parallel................. 33
2.11 Laboratory Task 3: Droop ........................ 34
2.12 Laboratory Task 4: Potentiometer (Pot)................. 36
2.13 Further Study: Thevenin s Theorem................... 39
References and Further Readings........................... 40
3 Diodes and Transistors................................. 41
3.1 Semiconductors................................. 41
3.2 Diodes....................................... 42
3.3 Zener Diode.................................... 45
3.4 Transistors..................................... 47
3.5 Operational Amplifier to Protect Your Circuit............ 52
3.6 Laboratory Task 1: LED........................... 54
x Contents
3.7 Laboratory Task 2: Zener Diode...................... 56
3.8 Laboratory Task 3: Transistor....................... 58
References and Further Readings........................... 61
4 Temperature Sensors.................................. 63
4.1 Thermocouple.................................. 63
4.2 Thermistor..................................... 65
4.3 Diode Temperature Sensor.......................... 66
4.4 Transistor Temperature Sensor....................... 68
4.5 Laboratory Task 1: Thermistor....................... 69
4.6 Laboratory Task 2: Zener Diode Temperature Sensor....... 71
4.7 Laboratory Task 3: Transistor Temperature Sensor......... 74
References and Further Readings........................... 78
5 Wheatstone Bridge.................................... 79
5.1 Wheatstone Bridge............................... 79
5.2 Strain Gauge................................... 81
5.3 Cantilever Biosensor.............................. 82
5.4 Laboratory Task 1: Wheatstone Bridge................. 84
5.5 Laboratory Task 2: Wheatstone Bridge for a Thermistor..... 85
5.6 Laboratory Task 3: Wheatstone Bridge for a Strain Gauge. ... 88
References and Further Readings........................... 90
6 Op-Amp........................................... 91
6.1 Op-Amp...................................... 91
6.2 Basics of Op-Amp............................... 91
6.3 Voltage Follower or Buffer Op-Amp................... 95
6.4 Non-Inverting Op-Amp............................ 95
6.5 Inverting Op-Amp............................... 97
6.6 Summing Op-Amp............................... 98
6.7 Differential Op-Amp.............................. 98
6.8 Laboratory Task 1: Non-inverting Op-Amp Operation....... 99
6.9 Laboratory Task 2: Signal Conditioning for Temperature
Sensor........................................ 102
6.10 Further Study: Op-Amp Filters....................... 106
References and Further Readings........................... 108
7 Light Sensors........................................ 109
7.1 Light......................................... 109
7.2 Photoresistor................................... 110
7.3 Photodiode.................................... 111
7.4 Phototransistor.................................. 115
7.5 Light-Emitting Diode (LED)........................ 116
7.6 Laser Diode.................................... 118
7.7 Laboratory Task 1: Photoconductive Operation............ 120
Contents xi
7.8 Laboratory Task 2: Photovoltaic Operation.............. 123
References and Further Readings........................... 126
8 Spectrophotometry.................................... 127
8.1 Spectrophotometry............................... 127
8.2 Spectrophotometry Biosensor Example: Pulse Oximeter...... 131
8.3 Miniature Spectrophotometer........................ 133
8.4 Optical Fibers.................................. 136
8.5 Laboratory Task 1: Hemoglobin Quantification
with a Spectrophotometer.......................... 140
8.6 Laboratory Task 2: Hemoglobin Quantification
with LED/PD Circuit............................. 146
8.7 Laboratory Task 3: Meat Quality Monitoring
with Reflection Probe............................. 148
References and Further Readings........................... 151
9 Fluorescence........................................ 153
9.1 Fluorescence................................... 154
9.2 Fluorescent Dyes................................ 156
9.3 Advanced Fluorescent Dyes: GFP, SYBR, and QD......... 159
9.4 Autofluorescence................................ 161
9.5 Detection of Fluorescence.......................... 163
9.6 Laboratory Task 1: 180° Back Scatter Fluorescence
Detection for Fluorescein........................... 165
9.7 Laboratory Task 2: 90° Side Scatter Fluorescence
Detection for Urine............................... 168
References and Further Readings........................... 170
10 Electrochemical Sensors................................ 171
10.1 Electrolytic and Electrochemical Cells.................. 171
10.2 Ion-Selective Electrodes (ISEs; Potentiometrie)............ 176
10.3 pH Electrode (Potentiometrie)....................... 178
10.4 Amperometric Biosensors.......................... 179
10.5 Conductometric Biosensors......................... 180
10.6 Laboratory Task 1: Buffer Preparations and Their pH
Measurements.................................. 182
10.7 Laboratory Task 2: pH Meter Circuit.................. 186
10.8 Laboratory Task 3: Fluoride Ion Selective Electrode
with PH Meter.................................. 189
10.9 Laboratory Task 4: Fluoride Ion Selective Electrode
with Circuit.................................... 191
References and Further Readings........................... 192
11 Piezoelectric Sensors................................... 195
11.1 Piezoelectricity.................................. 195
11.2 Pressure Sensors................................. 198
xü Contents
11.3 Crystal Oscillators............................... 198
11.4 Quartz Crystal Microbalance (QCM)................... 199
11.5 Viscoelasticity Consideration in QCM.................. 201
11.6 Flow Cell QCM as Biosensor....................... 203
11.7 Laboratory Task 1: Quantifying BSA Adsorption
on QCM Sensor................................. 204
References and Further Readings........................... 211
12 Glucose Sensors...................................... 213
12.1 Optical Glucose Sensor............................ 213
12.2 Electrochemical Glucose Sensor...................... 215
12.3 Other Electrochemical Biosensors..................... 218
12.4 Continuous Glucose Monitoring (CGM)................ 219
12.5 Laboratory Task 1: Glucose Assay Kit with a
Spectrophotometer............................... 221
12.6 Laboratory Task 2: Glucose Assay Kit
with LED/PD Circuit............................. 224
12.7 Laboratory Task 3: Commercial Electrochemical
Glucose Sensor................................. 226
References and Further Readings........................... 228
13 Immunosensors...................................... 229
13.1 Enzyme-Linked Immunosorbent Assay (ELISA)........... 229
13.2 Antibodies..................................... 232
13.3 Antibody Fragments and Aptamers.................... 234
13.4 Lateral-Flow Assay (LFA).......................... 236
13.5 Optical Immunosensors............................ 238
13.6 Surface Plasmon Resonance (SPR) Immunosensor......... 239
13.7 Electrochemical Immunosensors...................... 241
13.8 Impedance Immunosensors: Interdigitated Microelectrode
(IME) Immunosensor............................. 243
13.9 Piezoelectric Immunosensors: QCM Immunosensor......... 244
13.10 Immunosensing Kits Versus Handheld Immunosensors...... 245
13.11 Laboratory Task 1: Insulin ELISA Kit.................. 246
13.12 Laboratory Task 2: Insulin ELISA Kit with Smartphone
Camera....................................... 248
13.13 Laboratory Task 3: Pregnancy Test (LFA)
with Smartphone Camera........................... 251
13.14 Furfher Study: DNA Sensors........................ 254
References and Further Readings........................... 255
14 Lab-on-a-Chip Biosensors............................... 257
14.1 What Is Lab-on-a-Chip (LOC)?...................... 257
14.2 How to Make LOCs: Photolithography and Soft
Lithography.................................... 259
14.3 Early LOC: Capillary Electrophoresis (CE).............. 263
Contents xiii
14.4 LOCs for Point-of-Care Testing (POCT)................ 265
14.5 Use of Optical Fibers in LOCs....................... 267
14.6 Sample/Reagent Introduction........................ 269
14.7 Mixing in LOC................................. 270
14.8 Paper-Based LOCs............................... 272
14.9 LOC Sensing with a Smartphone Camera............... 273
14.10 Other Applications of LOCs........................ 274
14.11 Laboratory Task 1: Fabrication of LOC
by Soft Lithography.............................. 275
14.12 Laboratory Task 2: Mixing in LOC................... 277
14.13 Laboratory Task 3: Fabrication of a Paper-Based LOC...... 280
14.14 Laboratory Task 4: Bradford Assay
with Paper-Based LOC............................ 284
14.15 Further Study: Latex Immunoagglutination
Assay (LIA) in LOC.............................. 287
14.16 Further Study: Polymerase Chain Reaction (PCR)
in LOC....................................... 288
References and Further Readings........................... 293
15 Nanobiosensors...................................... 299
15.1 Gold Nanoparticles (AuNPs)........................ 299
15.2 Quantum Dots (QDs)............................. 303
15.3 Zinc Oxide (ZnO) Nanostructures..................... 305
15.4 Carbon Nanotubes (CNTs) and Graphene............... 307
15.5 Nanoporous Gold................................ 310
15.6 Concluding Remarks.............................. 310
References and Further Readings........................... 311
Appendix: Microcontroller................................. 313
Index................................................. 325
|
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| spelling | Yoon, Jeong-Yeol Verfasser (DE-588)1028530862 aut Introduction to biosensors from electric circuits to immunosensors Jeong-Yeol Yoon Second edition Schweiz Springer [2016] © 2016 xv, 331 Seiten Illustrationen, Diagramme (teilweise farbig) txt rdacontent n rdamedia nc rdacarrier Ingenieurwissenschaften Engineering Biomedical engineering Biophysics Biological physics Electronic circuits Nanotechnology Biosensor (DE-588)4193016-2 gnd rswk-swf (DE-588)4123623-3 Lehrbuch gnd-content Biosensor (DE-588)4193016-2 s DE-604 Erscheint auch als Online-Ausgabe 978-3-319-27413-3 https://doi.org/10.1007/978-3-319-27413-3 Resolving-System HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028753932&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Yoon, Jeong-Yeol Introduction to biosensors from electric circuits to immunosensors Ingenieurwissenschaften Engineering Biomedical engineering Biophysics Biological physics Electronic circuits Nanotechnology Biosensor (DE-588)4193016-2 gnd |
| subject_GND | (DE-588)4193016-2 (DE-588)4123623-3 |
| title | Introduction to biosensors from electric circuits to immunosensors |
| title_auth | Introduction to biosensors from electric circuits to immunosensors |
| title_exact_search | Introduction to biosensors from electric circuits to immunosensors |
| title_full | Introduction to biosensors from electric circuits to immunosensors Jeong-Yeol Yoon |
| title_fullStr | Introduction to biosensors from electric circuits to immunosensors Jeong-Yeol Yoon |
| title_full_unstemmed | Introduction to biosensors from electric circuits to immunosensors Jeong-Yeol Yoon |
| title_short | Introduction to biosensors |
| title_sort | introduction to biosensors from electric circuits to immunosensors |
| title_sub | from electric circuits to immunosensors |
| topic | Ingenieurwissenschaften Engineering Biomedical engineering Biophysics Biological physics Electronic circuits Nanotechnology Biosensor (DE-588)4193016-2 gnd |
| topic_facet | Ingenieurwissenschaften Engineering Biomedical engineering Biophysics Biological physics Electronic circuits Nanotechnology Biosensor Lehrbuch |
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