Rna-protein complexes and interactions: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
[2016]
|
| Schriftenreihe: | Methods in molecular biology
1421 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 332 Seiten Illustrationen |
| ISBN: | 9781493935895 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804175869888954368 |
|---|---|
| adam_text | Titel: RNA-protein complexes and interactions
Autor: Lin, Ren-Jang
Jahr: 2016
Contents
Dedication....................................................... v
Preface.......................................................... vii
Contributors.......................................................... xi
1 Characterization of RNA-Protein Interactions: Lessons
from Two RNA-Binding Proteins, SRSF1 and SRSF2................... 1
Lindsey Skrdlant and Ren-Jang Lin
2 Identification of mRNA-Interacting Factors by MS2-TRAP
(MS2-Tagged RNA Affinity Purification)............................ 15
Je-Hyun Toon and Myriam Gorospe
3 Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes
Assembled In Vitro............................................. 23
Shuai Hou, Lei Shi, and Haixin Lei
4 Detecting RNA-Protein Interaction Using End-Labeled Biotinylated
RNA Oligonucleotides and Immunoblotting.......................... 35
Xuexiu Zheng, Sunghee Cho, Heegyum Moon, Tiing Jen Loh, Ha Na Jang,
and Haihong Shen
5 Purification of RNA-Protein Splicing Complexes Using a Tagged Protein
from In Vitro Splicing Reaction Mixture............................. 45
Naoyuki Kataoka
6 Loading of Argonaute Protein with Small Duplex RNA
in Cellular Extracts............................................. 53
Keith T. Gagnon
7 In Vitro Analysis of Ribonucleoprotein Complex Remodeling
and Disassembly............................................... 69
Hua-Lin Zhou and Hua Lou
8 Single-Turnover Kinetics of Methyl Transfer to tRNA
by Methyltransferases........................................... 79
Ta-Ming Hou
9 Purification and Functional Reconstitution of Box H/ACA
Ribonucleoprotein Particles...................................... 97
Chao Huang, Guowei Wu, and Ti-Tao Tu
10 Northwestern Blot Analysis: Detecting RNA-Protein Interaction
After Gel Separation of Protein Mixture............................. Ill
Shangbing Zang and Ren-Jang Lin
11 Measuring mRNA Translation by Polysome Profiling................... 127
Marek Kudla and Pedor V. Karginov
12 Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq...... 137
Cheryl Stork and Sika Zheng
ix
X
Contents
13 Identification of Endogenous mRNA-Binding Proteins
in Yeast Using Crosslinking and PolyA Enrichment..................... 153
Sarah E Mitchell and Roy Parker
14 Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein
Interaction Networks........................................... 165
Hsin-Sung Teh, Jae-Woong Chang, andJeongsik Tong
15 Detection of Protein-Protein Interaction Within an RNA-Protein Complex
Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking......... 175
Fu-Lung Teh, Luh Tung, and Tien-Hsien Chang
16 Evolution of Cell-Type-Specific RNA Aptamers Via Live
Cell-Based SELEX............................................. 191
Jiehua Zhou and John J. Rossi
17 mCarts: Genome-Wide Prediction of Clustered Sequence Motifs
as Binding Sites for RNA-Binding Proteins........................... 215
Sebastien M. Weyn-Vanhentenryck and Chaolin Zhang
18 Design of RNA-Binding Proteins: Manipulate Alternative Splicing
in Human Cells with Artificial Splicing Factors........................ 227
Tang Wang and Zefeng Wang
19 Informational Suppression to Probe RNA:RNA Interactions in the Context
of Ribonucleoproteins: U1 and 5 Splice-Site Base-Pairing............... 243
Jiazi Tan and Xavier Roca
20 Analysis of Alternative Pre-RNA Splicing in the Mouse Retina
Using a Fluorescent Reporter..................................... 269
Daniel Murphy, Saravanan Kolandaivelu, Visvanathan Ramamurthy,
and Peter Stoilov
Index............................................................... 287
|
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| isbn | 9781493935895 |
| language | English |
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| physical | 332 Seiten Illustrationen |
| publishDate | 2016 |
| publishDateSearch | 2016 |
| publishDateSort | 2016 |
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| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Rna-protein complexes and interactions methods and protocols edited by Ren-Jang Lin, Irell & Manella Graduate School of Biological Science of the City of Hope, Duarte, CA, USA; Department of Molecular and Cellular Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA New York Humana Press [2016] 2016 332 Seiten Illustrationen txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1421 Springer protocols Lin, Ren-Jang edt Erscheint auch als Online-Ausgabe 978-1-4939-3591-8 Methods in molecular biology 1421 (DE-604)BV035362695 1421 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028746200&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rna-protein complexes and interactions methods and protocols Methods in molecular biology |
| title | Rna-protein complexes and interactions methods and protocols |
| title_auth | Rna-protein complexes and interactions methods and protocols |
| title_exact_search | Rna-protein complexes and interactions methods and protocols |
| title_full | Rna-protein complexes and interactions methods and protocols edited by Ren-Jang Lin, Irell & Manella Graduate School of Biological Science of the City of Hope, Duarte, CA, USA; Department of Molecular and Cellular Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA |
| title_fullStr | Rna-protein complexes and interactions methods and protocols edited by Ren-Jang Lin, Irell & Manella Graduate School of Biological Science of the City of Hope, Duarte, CA, USA; Department of Molecular and Cellular Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA |
| title_full_unstemmed | Rna-protein complexes and interactions methods and protocols edited by Ren-Jang Lin, Irell & Manella Graduate School of Biological Science of the City of Hope, Duarte, CA, USA; Department of Molecular and Cellular Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA |
| title_short | Rna-protein complexes and interactions |
| title_sort | rna protein complexes and interactions methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028746200&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
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