Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
Shaker Verlag
2015
|
| Schriftenreihe: | Chemical biotechnology
Volume 21 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | XV, 234 Seiten Illustrationen, Diagramme 21 cm |
| ISBN: | 9783844038842 3844038841 |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | VII
TABLE OF CONTENTS
DANKSAGUNG IV
SUMMARY IX
ZUSAMMENFASSUNG X
LIST OF ABBREVIATIONS XI
LIST OF SYMBOLS XIV
LIST OF CELLS XV
CHAPTER 1: INTRODUCTION 1
GENERAL INTRODUCTION 2
THE SINGLE CELL AS THE BASIC BIOLOGICAL UNIT 3
SINGLE-CELL ANALYSIS REQUIRES ENVIRONMENTAL CONTROL 4
PHYSIOLOGICAL CONSEQUENCES OF ENVIRONMENTAL GRADIENTS AND IMPLICATIONS
FOR MICROFLUIDIC
SINGLE-CELL ANALYSIS 7
THE SINGLE-CELL CONTINUUM 10
HYDRODYNAMICS SHAPE THE PHYSICAL ARCHITECTURE OF THE SINGLE-CELL
BOUNDARY ENVIRONMENT 11
MICROFLUIDIC SINGLE-CELL ANALYSIS ENABLES ENVIRONMENTAL CONTROL 14
SINGLE-CELL CULTIVATION IN CONTROLLED ENVIRONMENTS WITH THE ENVIROSTAT
16
THE CATALYTIC BLACK BOX: HOW TO ASSESS SINGLE CELLS IN A
BIOTECHNOLOGICAL CONTEXT? 20
CONCLUSIONS 22
SCOPE OF THE THESIS 23
CHAPTER 2: TIME-RESOLVED SINGLE-CELL ANALYSIS REVEALS ELEVATED SPECIFIC
GROWTH RATES OF ISOLATED
MICROBES AND MICROPOPULATIONS IN CONTROLLED ENVIRONMENTS 25
SUMMARY 26
INTRODUCTION 27
MATERIALS AND METHODS 29
RESULTS AND DISCUSSION 34
CONCLUSIONS 40
CHAPTER 3: MICROFLUIDIC CONTROL OF SINGLE YEAST MICROENVIRONMENTS
DISCLOSES ULTRASENSITIVITY OF
THE MOX PROMOTER TO CARBON CATABOLITE REPRESSION 41
SUMMARY 42
INTRODUCTION 43
MATERIALS AND METHODS 46
RESULTS 53
DISCUSSION 68
CONCLUSIONS 71
CHAPTER 4: QUANTITATIVE MEASUREMENT OF GLUCOSE UPTAKE AT A SINGLE-CELL
LEVEL 73
HTTP://D-NB.INFO/1075922291
VIII
SUMMARY 74
INTRODUCTION 75
MATERIALS AND METHODS 79
RESULTS 85
DISCUSSION 104
CONCLUSIONS 108
CHAPTER 5: THE FLUX PROBE CONCEPT - SECRETED PROTEIN AS A NON-DISRUPTIVE
INFORMATION CARRIER FOR
13
C-BASED METABOLIC FLUX ANALYSIS ILL
SUMMARY 112
INTRODUCTION 113
MATERIALS AND METHODS 116
RESULTS 121
DISCUSSION 135
CONCLUSIONS 138
CHAPTER 6: TECHNICAL BIAS OF MICROCULTIVATION ENVIRONMENTS ON
SINGLE-CELL PHYSIOLOGY 139
SUMMARY 140
INTRODUCTION 141
MATERIALS AND METHODS 146
RESULTS 149
DISCUSSION 157
CONCLUSIONS 160
CHAPTER 7: GENERAL DISCUSSION 161
INTRODUCTION 162
BEYOND THE BULK: ANALYTICAL CONCEPTS FOR SINGLE-CELL ANALYSIS 163
PIVOTAL DESCRIPTORS FOR SINGLE-CELL PHYSIOLOGY: LINKING CELL AND
ENVIRONMENT 175
CONCLUSIONS 188
CHAPTER 8: CONCLUDING REMARKS AND OUTLOOK 189
CHALLENGING BIOLOGICAL LIMITS WITH MICROFLUIDIC SINGLE-CELL ANALYSIS 190
OUTLOOK 193
CHAPTER 9: APPENDIX 195
CHAPTER 10: REFERENCES 207
CURRICULUM VITAE 229
|
| any_adam_object | 1 |
| author | Dusny, Christian |
| author_GND | (DE-588)1048208133 |
| author_facet | Dusny, Christian |
| author_role | aut |
| author_sort | Dusny, Christian |
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| bvnumber | BV043325638 |
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| dewey-full | 660.62 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.62 |
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| dewey-sort | 3660.62 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| format | Thesis Book |
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| indexdate | 2024-07-10T07:23:08Z |
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| isbn | 9783844038842 3844038841 |
| language | English |
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| spelling | Dusny, Christian Verfasser (DE-588)1048208133 aut Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments Christian Dusny Aachen Shaker Verlag 2015 XV, 234 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Chemical biotechnology Volume 21 Dissertation Technische Universität Dortmund 2015 Zellwachstum (DE-588)4190674-3 gnd rswk-swf Einzelzellanalyse (DE-588)7657465-9 gnd rswk-swf Katalytische Aktivität (DE-588)4279994-6 gnd rswk-swf Bakterien (DE-588)4004296-0 gnd rswk-swf Extrazellulärraum (DE-588)4205506-4 gnd rswk-swf Hefeartige Pilze (DE-588)4230509-3 gnd rswk-swf Lab on a Chip (DE-588)7610677-9 gnd rswk-swf chemical biotechnology Envirostat Single cell analysis (DE-588)4113937-9 Hochschulschrift gnd-content Bakterien (DE-588)4004296-0 s Hefeartige Pilze (DE-588)4230509-3 s Zellwachstum (DE-588)4190674-3 s Katalytische Aktivität (DE-588)4279994-6 s Extrazellulärraum (DE-588)4205506-4 s Einzelzellanalyse (DE-588)7657465-9 s Lab on a Chip (DE-588)7610677-9 s DE-604 Chemical biotechnology Volume 21 (DE-604)BV026780082 21 B:DE-101 application/pdf http://d-nb.info/1075922291/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028745945&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dusny, Christian Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments Chemical biotechnology Zellwachstum (DE-588)4190674-3 gnd Einzelzellanalyse (DE-588)7657465-9 gnd Katalytische Aktivität (DE-588)4279994-6 gnd Bakterien (DE-588)4004296-0 gnd Extrazellulärraum (DE-588)4205506-4 gnd Hefeartige Pilze (DE-588)4230509-3 gnd Lab on a Chip (DE-588)7610677-9 gnd |
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| title | Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments |
| title_auth | Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments |
| title_exact_search | Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments |
| title_full | Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments Christian Dusny |
| title_fullStr | Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments Christian Dusny |
| title_full_unstemmed | Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments Christian Dusny |
| title_short | Microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments |
| title_sort | microfluidics enable quantitative physiology of individual microorganisms in controlled extracellular environments |
| topic | Zellwachstum (DE-588)4190674-3 gnd Einzelzellanalyse (DE-588)7657465-9 gnd Katalytische Aktivität (DE-588)4279994-6 gnd Bakterien (DE-588)4004296-0 gnd Extrazellulärraum (DE-588)4205506-4 gnd Hefeartige Pilze (DE-588)4230509-3 gnd Lab on a Chip (DE-588)7610677-9 gnd |
| topic_facet | Zellwachstum Einzelzellanalyse Katalytische Aktivität Bakterien Extrazellulärraum Hefeartige Pilze Lab on a Chip Hochschulschrift |
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| volume_link | (DE-604)BV026780082 |
| work_keys_str_mv | AT dusnychristian microfluidicsenablequantitativephysiologyofindividualmicroorganismsincontrolledextracellularenvironments |
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