Transposons and retrotransposons: methods and protocols
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
[2016]
|
| Schriftenreihe: | Methods in molecular biology
1400 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xii, 434 Seiten Illustrationen, Diagramme |
| ISBN: | 9781493933709 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804175869226254336 |
|---|---|
| adam_text | Titel: Transposons and retrotransposons
Autor: García-Pérez, José L
Jahr: 2016
Contents
Preface.......................................................... v
Contributors...................................................... ix
1 Study of Transposable Elements and Their Genomic Impact.............. 1
Martin Munoz-Lopez, Raquel Vilar-Astasio, Pablo Tristan-Ramos,
Cesar Lopez-Ruiz, and Jose L. Garcia-Perez
2 Bacterial Group II Introns: Identification and Mobility Assay............. 21
Nicolds Toro, Maria Dolores Molina-Sdnchez, Rafael Nisa-Martinez,
Francisco Martinez-Abarca, and Fernando Manuel Garcia-Rodriguez
3 In Silico Methods to Identify Exapted Transposable Element Families....... 33
LeeAnn Ramsay and Guillaume Bourque
4 Retrotransposon Capture Sequencing (RC-Seq): A Targeted, High-Throughput
Approach to Resolve Somatic LI Retrotransposition in Humans........... 47
Francisco J, Sanchez-Luque, Sandra R. Richardson,
and Geoffrey J. Faulkner
5 Long Interspersed Element Sequencing (Ll-Seq): A Method
to Identify Somatic LINE-1 Insertions in the Human Genome............ 79
Tara T. Doucet and Haig H. Kazazian Jr.
6 Combining Amplification Typing of LI Active Subfamilies (ATLAS)
with High-Throughput Sequencing................................ 95
Raheleh Rahbari and Richard M. Badge
7 RNA-Seq Analysis to Measure the Expression of SINE Retroelements....... 107
Angel Carlos Romdn, Antonio Morales-Herndndez,
and Pedro M. Ferndndez-Salguero
8 Qualitative and Quantitative Assays of Transposition and Homologous
Recombination of the Retrotransposon Tfl in Schizosaccharomyces pombe .... 117
Maya Sangesland, Angela Atwood-Moore, Sudhir K. Rai,
and Henry L. Levin
9 LINE Retrotransposition Assays in Saccharomyces cerevisiae............... 131
Axel V. Horn and Jeffrey S. Han
10 LINE-1 Cultured Cell Retrotransposition Assay....................... 139
Huira C. Kopera, Peter A. Larson, John B. Moldovan,
Sandra R. Richardson, Ting Liu, and John V. Moran
11 LI Retrotransposition in Neural Progenitor Cells...................... 157
Alysson R. Muotri
12 Characterization of Engineered LI Retrotransposition Events:
The Recovery Method.......................................... 165
David Cano, Santiago Morell, Andres J. Pulgarin, Suyapa Amador,
and Jose L. Garcia-Perez
vii
viii Contents
13 SINE Retrotransposition: Evaluation of Alu Activity
and Recovery of De Novo Inserts.................................. 183
Catherine Ade and Astrid M. Roy-Engel
14 The Engineered SVA Trawr-mobilization Assay........................ 203
Anja Bock and Gerald G. Schumann
15 Detection of LINE-1 RNAs by Northern Blot........................ 223
Prescott Deininger and Victoria P. Belancio
16 Monitoring Long Interspersed Nuclear Element 1 Expression
During Mouse Embryonic Stem Cell Differentiation.................... 237
Maxime Bodak and Constance Ciaudo
17 Immunodetection of Human LINE-1 Expression
in Cultured Cells and Human Tissues............................... 261
Reema Sharma, Nemanja Rodic, Kathleen H. Burns,
and Martin S. Taylor
18 Cellular Localization of Engineered Human LINE-1 RNA and Proteins..... 281
Aurelien J. Doucet, Euginia Basyuk, and Nicolas Gilbert
19 Purification of Ll-Ribonucleoprotein Particles (Ll-RNPs)
from Cultured Human Cells...................................... 299
PrabhatK. Mandal and HaigH. Kazazian Jr.
20 Characterization of Ll-Ribonucleoprotein Particles..................... 311
Martin S. Taylor, John LaCava, Lixin Dai, Paolo Mita,
Kathleen H. Burns, Michael P. Rout, and JefD. Boeke
21 LEAP: LI Element Amplification Protocol........................... 339
Huira C. Kopera, Diane A. Flasch, Mitsuhiro Nakamura,
Tomoichiro Miyoshi, Aurelien J. Doucet, and John V. Moran
22 Biochemical Approaches to Study LINE-1 Reverse Transcriptase
Activity In Vitro............................................... 357
Sebastien Viollet, AurHien J. Doucet, and Gael Cristofari
23 Methylated DNA Immunoprecipitation Analysis of Mammalian
Endogenous Retroviruses........................................ 377
Rita Rebollo and Dixie L. Mager
24 Profiling DNA Methylation and Hydroxymethylation
at Retrotransposable Elements.................................... 387
Lorenzo de la Rica, Jatinder S. Stanley, and Miguel R. Branco
25 A Large-Scale Functional Screen to Identify Epigenetic Repressors
of Retrotransposon Expression.................................... 403
Gabriela Ecco, Helen M. Rowe, and Didier Trono
26 Reprogramming of Human Fibroblasts to Induced Pluripotent Stem
Cells with Sleeping Beauty Transposon-Based Stable Gene Delivery......... 419
Attila Sebe and ZoltAn Ivies
Index........................................................... 429
|
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| indexdate | 2024-07-10T07:23:08Z |
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| isbn | 9781493933709 |
| language | English |
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| physical | xii, 434 Seiten Illustrationen, Diagramme |
| publishDate | 2016 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | García Peréz, José L. Verfasser aut Transposons and retrotransposons methods and protocols New York Humana Press [2016] xii, 434 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1400 Methods in molecular biology 1400 (DE-604)BV035362695 1400 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028745785&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | García Peréz, José L. Transposons and retrotransposons methods and protocols Methods in molecular biology |
| title | Transposons and retrotransposons methods and protocols |
| title_auth | Transposons and retrotransposons methods and protocols |
| title_exact_search | Transposons and retrotransposons methods and protocols |
| title_full | Transposons and retrotransposons methods and protocols |
| title_fullStr | Transposons and retrotransposons methods and protocols |
| title_full_unstemmed | Transposons and retrotransposons methods and protocols |
| title_short | Transposons and retrotransposons |
| title_sort | transposons and retrotransposons methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028745785&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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