The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy: = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Würzburg
2015
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| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | 91 Blätter Illustrationen, Diagramme |
Internformat
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| 245 | 1 | 0 | |a The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy |b = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie |c submitted by Dennis Klein from Alzenau i. Ufr. |
| 246 | 1 | 1 | |a Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie |
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Datensatz im Suchindex
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| adam_text | THE PATHOGENIC ROLE OF ENDOGENOUS ANTIBODIES IN A MOUSE MODEL FOR
CHARCOT-MARIE-TOOTH IB NEUROPATHY
DIE PATHOGENETISCHE FUNKTION VON ENDOGENEN ANTIKORPERN IN EINEM
MAUS-MODELL DER CHARCOT-MARIE-TOOTH IB NEUROPATHIE
DOCTORAL THESIS FOR
A DOCTORAL DEGREE
AT THE GRADUATE
SCHOOL OF LIFE SCIENCES,
JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG,
SECTION NEUROSCIENCE
SUBMITTED BY
DENNIS KLEIN
FROM
ALZENAU I. UFR.
WURZBURG 2015
HTTP://D-NB.INFO/1081097450
4
TABLE OF CONTENTS
1. ABSTRACT 6
2. ZUSAMMENFASSUNG 7
3. INTRODUCTION 9
3.1. CHARCOT-MARIE-TOOTH NEUROPATHIES 9
3.2. RODENT MODELS FOR CMT1 10
3.3. LOW GRADE INFLAMMATION AS AN IMPORTANT AMPLIFIER IN THE
PATHOGENESIS OF
CMT1 11
3.3.1. THE INNATE IMMUNE SYSTEM 11
3.3.2. THE ADAPTIVE IMMUNE
SYSTEM 13
3.4. AIM OF THE STUDY 15
4. MATERIAL AND METHODS 16
4.1. EQUIPMENT, BUFFERS
AND SOLUTIONS, ANTIBODIES, AND PRIMER SEQUENCES 16
4.2. ANIMALS AND
GENOTYPING : 16
4.3. SCIATIC NERVE
CRUSH INJURY 16
4.4. DISSECTION AND TISSUE PROCESSING 17
4.5. IMMUNOHISTOCHEMISTRY 18
4.6. RNA
EXTRACTION AND SEMI-QUANTITATIVE REAL-TIME PCR 20
4.7. WESTERN BLOT ANALYSIS 21
4.8. ELECTRON MICROSCOPY AND
MORPHOLOGICAL ANALYSIS 22
4.9. RECONSTITUTION EXPERIMENTS 22
4.10. NERVE CONDUCTION STUDIES 22
4.11. STATISTICAL ANALYSIS 23
5. RESULTS 24
5.1. ANTIBODIES ACCUMULATE IN PERIPHERAL NERVES
OF POHET MYELIN MUTANT MICE 24
5.2. LACK OF EVIDENCE FOR COMPLEMENT DEPOSITION 28
5.3. REDUCED MACROPHAGE NUMBERS IN PERIPHERAL NERVES OF 6-MONTH-OLD
POHET JHD-/- MICE 30
5.4. JHD
DEFICIENCY AMELIORATES THE DEMYELINATING PHENOTYPE IN 6-MONTH-OLD
POHET MUTANT MICE 34
5.5. PASSIVE TRANSFER OF ANTIBODIES REVERTS
THE AMELIORATED DEMYELINATING
PHENOTYPE IN 6-MONTH-OLD POHET JHD-/- MICE 35
5
5.6. INCREASED MACROPHAGE NUMBERS IN PERIPHERAL NERVES OF 12-MONTH-OLD
POHETJHD-/- 38
5.7. JHD
DEFICIENCY AGGRAVATES THE DEMYELINATING PHENOTYPE IN 12-MONTH-OLD
POHET MUTANT MICE 40
5.8. ALTERED NUMBERS OF IMMUNE-RELATED
CELLS IN 12-MONTH-OLD POHET JHD-/-
MICE .45
6. DISCUSSION 52
6.1. ENDOGENOUS ANTIBODIES ACCUMULATE IN PERIPHERAL NERVES
OF POHET MYELIN
MUTANT MICE 52
6.2. CRUCIAL ROLE FOR
ENDOGENOUS ANTIBODIES IN EARLY MACROPHAGE-MEDIATED
DEMYELINATION 54
F
6.3. JHD
DEFICIENCY TRIGGERS INCREASED IMMUNE REACTIONS AND DISEASE AGGRAVATION
IN 12-MONTH-OLD POHET MICE 56
6.4. THE IMMUNE SYSTEM AS A THERAPEUTIC TARGET FOR CMT1 60
6.5. SYNOPSIS 62
7. REFERENCES 64
8 APPENDICES 74
8.1 TECHNICAL EQUIPMENT 74
8.2. REAGENTS 75
8.3. BUFFERS AND SOLUTIONS 77
8.4. PRIMER SEQUENCES FOR GENOTYPING 80
8.5. ANTIBODIES USED IN IMMUNOHISTOCHEMISTRY 81
9. ABBREVIATIONS 83
10. CURRICULUM
VITAE 86
11. PUBLICATIONS 87
12. DANKSAGUNG 90
|
| any_adam_object | 1 |
| author | Klein, Dennis 1985- |
| author_GND | (DE-588)1091569274 |
| author_facet | Klein, Dennis 1985- |
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| author_sort | Klein, Dennis 1985- |
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| dewey-full | 570 610 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 570 - Biology 610 - Medicine and health |
| dewey-raw | 570 610 |
| dewey-search | 570 610 |
| dewey-sort | 3570 |
| dewey-tens | 570 - Biology 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| spelling | Klein, Dennis 1985- Verfasser (DE-588)1091569274 aut The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie submitted by Dennis Klein from Alzenau i. Ufr. Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie Würzburg 2015 91 Blätter Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Dissertation Julius-Maximilians-Universität Würzburg 2015 Zusammenfassung in deutscher und englischer Sprache Antikörper (DE-588)4002290-0 gnd rswk-swf Charcot-Marie-Syndrom (DE-588)4259126-0 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Immunsystem (DE-588)4026643-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Maus (DE-588)4169148-9 s Charcot-Marie-Syndrom (DE-588)4259126-0 s Immunsystem (DE-588)4026643-6 s Antikörper (DE-588)4002290-0 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-121941 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-121941 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028740899&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Klein, Dennis 1985- The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie Antikörper (DE-588)4002290-0 gnd Charcot-Marie-Syndrom (DE-588)4259126-0 gnd Maus (DE-588)4169148-9 gnd Immunsystem (DE-588)4026643-6 gnd |
| subject_GND | (DE-588)4002290-0 (DE-588)4259126-0 (DE-588)4169148-9 (DE-588)4026643-6 (DE-588)4113937-9 |
| title | The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie |
| title_alt | Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie |
| title_auth | The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie |
| title_exact_search | The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie |
| title_full | The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie submitted by Dennis Klein from Alzenau i. Ufr. |
| title_fullStr | The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie submitted by Dennis Klein from Alzenau i. Ufr. |
| title_full_unstemmed | The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie submitted by Dennis Klein from Alzenau i. Ufr. |
| title_short | The pathogenic role of endogenous antibodies in a mouse model for Charcot-Marie-Tooth 1B neuropathy |
| title_sort | the pathogenic role of endogenous antibodies in a mouse model for charcot marie tooth 1b neuropathy die pathogenische funktion von endogenen antikorpern in einem maus modell der charcot marie tooth 1b neuropathie |
| title_sub | = Die pathogenische Funktion von endogenen Antikörpern in einem Maus-Modell der Charcot-Marie-Tooth 1B Neuropathie |
| topic | Antikörper (DE-588)4002290-0 gnd Charcot-Marie-Syndrom (DE-588)4259126-0 gnd Maus (DE-588)4169148-9 gnd Immunsystem (DE-588)4026643-6 gnd |
| topic_facet | Antikörper Charcot-Marie-Syndrom Maus Immunsystem Hochschulschrift |
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