Protein phosphorylation: a practical approach
Gespeichert in:
Bibliographische Detailangaben
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: London Oxford University Press 1999
Ausgabe:2nd ed
Schriftenreihe:Practical approach series 211
Schlagworte:
Online-Zugang:FAW01
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Volltext
Beschreibung:Includes bibliographical references and index
Study of protein phosphorylation in intact cells -- Analysis of signal transduction pathways using protein kinase inhibitors and activators -- Analysis of signal transduction pathways using protein-serin/threonine phosphatase inhibitors -- Analysis of signal transduction pathways using molecular biological manipulations -- Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates -- Phosphorylation site analysis by mass spectrometry -- Assay and purification of protein (serine/threonine) phosphatases -- Identification of substrates for protein-tyrosine phosphateases -- Assay and purification of protein-serine/threonine kinases -- Assay of protein kinases and phosphatases using specific peptide substrates -- Cloning and expression of cDNAs encoding protein kinase subunits -- Interaction cloning of protein kinase partners -- Identifying protein kinase substrates by expression screening with solid-phase phosphorylation -- Analysis of protein kinase interactions using the two-hybrid method -- Analysis of protein kinase interactions using biomolecular interaction analysis -- Analysis of protein kinase specificity using oriented peptide libraries -- Crystallization of protein kinases and phosphatases
Beschreibung:1 Online-Ressource (xxviii, 431 pages)
ISBN:0585483930
9780585483931

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