PCR 3: PCR in situ hybridization : practical approach
Gespeichert in:
| Format: | Elektronisch E-Book |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Oxford
IRL Press at Oxford University Press
©1998
|
| Schriftenreihe: | Practical approach series
186 |
| Schlagworte: | |
| Online-Zugang: | FAW01 FAW02 Volltext |
| Beschreibung: | Includes bibliographical references and index PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads.; There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules |
| Beschreibung: | 1 Online-Ressource (xviii, 205 pages) |
| ISBN: | 0585484163 1280375418 9780585484167 9781280375415 |
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Datensatz im Suchindex
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| dewey-full | 572.8 |
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| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8 |
| dewey-search | 572.8 |
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| discipline | Biologie |
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| id | DE-604.BV043073211 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T07:16:36Z |
| institution | BVB |
| isbn | 0585484163 1280375418 9780585484167 9781280375415 |
| language | English |
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| physical | 1 Online-Ressource (xviii, 205 pages) |
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| publishDate | 1998 |
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| publisher | IRL Press at Oxford University Press |
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| series2 | Practical approach series |
| spelling | PCR 3 PCR in situ hybridization : practical approach edited by C. Simon Herrington and John J. O'Leary PCR in situ hybridization PCR three Oxford IRL Press at Oxford University Press ©1998 1 Online-Ressource (xviii, 205 pages) txt rdacontent c rdamedia cr rdacarrier Practical approach series 186 Includes bibliographical references and index PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads.; There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules Polymerase Chain Reaction / laboratory manuals Situ Hybridization / laboratory manuals SCIENCE / Life Sciences / Molecular Biology bisacsh In situ hybridization fast Polymerase chain reaction fast Polymerase chain reaction cct In situ hybridization cct In situ hybridization Laboratory manuals Polymerase chain reaction Laboratory manuals Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf In-situ-Hybridisierung (DE-588)4215968-4 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s In-situ-Hybridisierung (DE-588)4215968-4 s 1\p DE-604 Herrington, C. S. Sonstige oth O'Leary, John J. Sonstige oth http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=98469 Aggregator Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | PCR 3 PCR in situ hybridization : practical approach Polymerase Chain Reaction / laboratory manuals Situ Hybridization / laboratory manuals SCIENCE / Life Sciences / Molecular Biology bisacsh In situ hybridization fast Polymerase chain reaction fast Polymerase chain reaction cct In situ hybridization cct In situ hybridization Laboratory manuals Polymerase chain reaction Laboratory manuals Polymerase-Kettenreaktion (DE-588)4256726-9 gnd In-situ-Hybridisierung (DE-588)4215968-4 gnd |
| subject_GND | (DE-588)4256726-9 (DE-588)4215968-4 |
| title | PCR 3 PCR in situ hybridization : practical approach |
| title_alt | PCR in situ hybridization PCR three |
| title_auth | PCR 3 PCR in situ hybridization : practical approach |
| title_exact_search | PCR 3 PCR in situ hybridization : practical approach |
| title_full | PCR 3 PCR in situ hybridization : practical approach edited by C. Simon Herrington and John J. O'Leary |
| title_fullStr | PCR 3 PCR in situ hybridization : practical approach edited by C. Simon Herrington and John J. O'Leary |
| title_full_unstemmed | PCR 3 PCR in situ hybridization : practical approach edited by C. Simon Herrington and John J. O'Leary |
| title_short | PCR 3 |
| title_sort | pcr 3 pcr in situ hybridization practical approach |
| title_sub | PCR in situ hybridization : practical approach |
| topic | Polymerase Chain Reaction / laboratory manuals Situ Hybridization / laboratory manuals SCIENCE / Life Sciences / Molecular Biology bisacsh In situ hybridization fast Polymerase chain reaction fast Polymerase chain reaction cct In situ hybridization cct In situ hybridization Laboratory manuals Polymerase chain reaction Laboratory manuals Polymerase-Kettenreaktion (DE-588)4256726-9 gnd In-situ-Hybridisierung (DE-588)4215968-4 gnd |
| topic_facet | Polymerase Chain Reaction / laboratory manuals Situ Hybridization / laboratory manuals SCIENCE / Life Sciences / Molecular Biology In situ hybridization Polymerase chain reaction In situ hybridization Laboratory manuals Polymerase chain reaction Laboratory manuals Polymerase-Kettenreaktion In-situ-Hybridisierung |
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